IF (Immunofluorescence)
(Immunofluorescence staining of HepG2 cells with AAA235549 at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
GPC3 recombinant antibody
GPC3 Antibody
Gene Names
GPC3; SGB; DGSX; MXR7; SDYS; SGBS; OCI-5; SGBS1; GTR2-2
Reactivity
Human
Applications
Immunofluorescence, Western Blot, ELISA
Purity
Affinity-Chromatography
Synonyms
GPC3, Recombinant Antibody; GPC3 Antibody; Glypican-3; GTR2-2; Intestinal protein OCI-5; MXR7; Secreted glypican-3; GPC3; OCI5; GPC3 recombinant antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG
Clone Number
4H11
Purity/Purification
Affinity-Chromatography
Form/Format
Liquid; In phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
603
Applicable Applications for GPC3 recombinant antibody
IF (Immunofluorescence), WB (Western Blot), ELISA
Immunogen Species
Human
Immunogen
A synthesized peptide derived from human GPC3
Research Area
Stem Cells
Preparation and Storage
Upon receipt, store at-20 degree C or-80 degree C. Avoid repeated freeze.
IF (Immunofluorescence)
(Immunofluorescence staining of HepG2 cells with AAA235549 at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
IF (Immunofluorescence)
(Immunofluorescence staining of HepG2 cells with AAA235549 at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
WB (Western Blot)
(Western BlotPositive WB detected in: 293 whole cell lysate, K562 whole cell lysateAll lanes: GPC3 antibody at 0.9ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 66, 60, 69 KDaObserved band size: 66 KDa)
WB (Western Blot)
(Western BlotPositive WB detected in: 293 whole cell lysate, K562 whole cell lysateAll lanes: GPC3 antibody at 0.9ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 66, 60, 69 KDaObserved band size: 66 KDa)
Related Product Information for GPC3 recombinant antibody
Cell surface proteoglycan that bears heparan sulfate. Inhibits the dipeptidyl peptidase activity of DPP4. May be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs. May play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. May regulate growth and tumor predisposition.
Description: The GPC3 recombinant monoclonal antibody is prepared by cloning the gene fragment encoding human GPC3 protein into the expression vector and subsequently transfecting clones into the cell line for in vitro expression. This GPC3 antibody can detect the endogenous content of the human GPC3 protein. It is purified by the affinity chromatography method. And it has been validated in ELISA, WB, and IF applications.
GPC3 is a cell-surface glycoprotein that exerts important functions in cellular signaling including cell growth, embryogenesis, and differentiation. GPC3 has been associated with hepatocellular carcinoma and a few other cancers.
Description: The GPC3 recombinant monoclonal antibody is prepared by cloning the gene fragment encoding human GPC3 protein into the expression vector and subsequently transfecting clones into the cell line for in vitro expression. This GPC3 antibody can detect the endogenous content of the human GPC3 protein. It is purified by the affinity chromatography method. And it has been validated in ELISA, WB, and IF applications.
GPC3 is a cell-surface glycoprotein that exerts important functions in cellular signaling including cell growth, embryogenesis, and differentiation. GPC3 has been associated with hepatocellular carcinoma and a few other cancers.
Product Categories/Family for GPC3 recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
glypican-3 isoform 1
NCBI Official Synonym Full Names
glypican 3
NCBI Official Symbol
GPC3
NCBI Official Synonym Symbols
SGB; DGSX; MXR7; SDYS; SGBS; OCI-5; SGBS1; GTR2-2
NCBI Protein Information
glypican-3
UniProt Protein Name
Glypican-3
UniProt Gene Name
GPC3
UniProt Synonym Gene Names
OCI5
Similar Products
Product Notes
The GPC3 gpc3 (Catalog #AAA235549) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GPC3 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GPC3 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the GPC3 gpc3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GPC3, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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