IF (Immunofluorescence)
(ICC/IF analysis of GRP94 in U87MG cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GRP94 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Mouse anti-Human GRP94 Monoclonal Antibody | anti-GRP94 antibody
GRP94 antibody
Gene Names
HSP90B1; ECGP; GP96; TRA1; GRP94; HEL35; HEL-S-125m
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunocytochemistry, Western Blot, ELISA
Purity
By protein-A affinity chromatography
Synonyms
GRP94, Antibody; GRP94 antibody; 94 kDa glucose-regulated protein; HSP90B1; GP96; TRA1; Tumor rejection antigen 1; GRP94; 94 kDa glucose-regulated protein 94 kDa glucose regulated protein; ECGP; Endoplasmin; Endothelial cell (HBMEC) glycoprotein; Glucose regulated protein 94kDa; gp96; gp96 homolog; GRP 9; Heat shock protein 90 kDa beta member 1; Stress inducible tumor rejection antigen GP96; Tumor rejection antigen gp96.; anti-GRP94 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b, k
Clone Number
AT94B9
Purity/Purification
By protein-A affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
803
Applicable Applications for anti-GRP94 antibody
FCM/FACS (Flow Cytometry), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot), ELISA
Antigen Species
Human
Immunogen
Recombinant human GRP94 (22-803aa) purified from E Coli.
Clone Note
Anti-human GRP94 mAb, clone AT94B9, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human GRP94 protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
IF (Immunofluorescence)
(ICC/IF analysis of GRP94 in U87MG cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GRP94 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
IF (Immunofluorescence)
(ICC/IF analysis of GRP94 in U87MG cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GRP94 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
WB (Western Blot)
(The Recombinant Human GRP94 (25ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GRP94 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant Human GRP94 Lane 2.: HeLa cell lysate Lane 3.: Jurkat cell lysate Lane 4.: HepG2 cell lysate)
WB (Western Blot)
(The Recombinant Human GRP94 (25ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GRP94 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant Human GRP94 Lane 2.: HeLa cell lysate Lane 3.: Jurkat cell lysate Lane 4.: HepG2 cell lysate)
Related Product Information for anti-GRP94 antibody
GRP94, also known as Heat shock protein 90kDa beta, member 1 (HSP90B1), is an abundant resident endoplasmic reticulum (ER) lumenal stress protein which together with cytosolic Hsp90 belongs to the Hsp90 family of molecular chaperones. It plays an important role in maintaining protein homeostasis in the secretory pathway. Also, GRP94 can function in the intracellular trafficking of peptides from the extracellular space to the MHC class I antigen processing pathway of antigen presentation cells.
Product Categories/Family for anti-GRP94 antibody
References
Randow F., et al. (2001) Nat Cell Biol. 3 (10): 231; Li Z., et al. (2002) Front Biosci. 7: 731-751
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
92,469 Da
NCBI Official Full Name
endoplasmin
NCBI Official Synonym Full Names
heat shock protein 90 beta family member 1
NCBI Official Symbol
HSP90B1
NCBI Official Synonym Symbols
ECGP; GP96; TRA1; GRP94; HEL35; HEL-S-125m
NCBI Protein Information
endoplasmin
UniProt Protein Name
Endoplasmin
UniProt Gene Name
HSP90B1
UniProt Synonym Gene Names
GRP94; TRA1; GRP-94
Similar Products
Product Notes
The GRP94 hsp90b1 (Catalog #AAA48190) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The GRP94 antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GRP94 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the GRP94 hsp90b1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GRP94, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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