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WB (Western Blot)
(Applications: Western BlotSample: Cell lysateDetection: Enhanced chemiluminescence"This is an excellent antibody to endoplasmin in HC11 cells. Clean, reliable detection with very little if any background. Great antibody for Westerns. Could probably be optimized for 1 hour primary incubation.")
Rabbit GRP94 Polyclonal Antibody | anti-HSP90B1 antibody
Anti-GRP94 antibody
Gene Names
HSP90B1; ECGP; GP96; TRA1; GRP94; HEL35; HEL-S-125m
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunohistochemistry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Synonyms
GRP94, Antibody; Anti-GRP94 antibody; Endoplasmin; heat shock protein 90kDa beta(Grp94), member 1; 94 kDa glucose regulated protein antibody; 94 kDa glucose-regulated protein antibody; ECGP antibody; Endoplasmin antibody; Endothelial cell(HBMEC) glycoprotein antibody; ENPL_HUMAN antibody; Glucose regulated protein 94 antibody; Glucose regulated protein 94kDa antibody; gp96 antibody; gp96 homolog antibody; GRP 94 antibody; GRP-94 antibody; Heat shock protein 90 kDa beta member 1 antibody; heat shock protein 90kDa beta(Grp94), member 1 antibody; Heat shock protein, 90 kDa, beta, 1 antibody; HSP90B1 antibody; Stress inducible tumor rejection antigen GP96 antibody; TRA1 antibody; tumor rejection antigen(gp96) 1 antibody; Tumor rejection antigen 1 antibody; Tumor rejection antigen gp96 antibody; Tumor rejection antigen-1(gp96) antibody; anti-HSP90B1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Sequence Length
803
Applicable Applications for anti-HSP90B1 antibody
ICC (Immunocytochemistry), IHC (Immunohistochemistry), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human GRP94 (687-700aa INPRHPLIRDMLRR), identical to the related mouse and rat sequences.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
WB (Western Blot)
(Applications: Western BlotSample: Cell lysateDetection: Enhanced chemiluminescence"This is an excellent antibody to endoplasmin in HC11 cells. Clean, reliable detection with very little if any background. Great antibody for Westerns. Could probably be optimized for 1 hour primary incubation.")
WB (Western Blot)
(Applications: Western BlotSample: Cell lysateDetection: Enhanced chemiluminescence"This is an excellent antibody to endoplasmin in HC11 cells. Clean, reliable detection with very little if any background. Great antibody for Westerns. Could probably be optimized for 1 hour primary incubation.")
IHC (Immunohistochemistry)
(Anti-GRP94 antibody, AAA45169, IHC(P)IHC(P): Rat Brain Tissue)
IHC (Immunohistochemistry)
(Anti-GRP94 antibody, AAA45169, IHC(P)IHC(P): Rat Brain Tissue)
IHC (Immunohistochemisry)
(Anti-GRP94 antibody, AAA45169, IHC(P)IHC(P): Human Lung Cancer Tissue)
IHC (Immunohistochemisry)
(Anti-GRP94 antibody, AAA45169, IHC(P)IHC(P): Human Lung Cancer Tissue)
IHC (Immunohiostchemistry)
(Anti-GRP94 antibody, AAA45169, IHC(P)IHC(P): HELA Cell)
IHC (Immunohiostchemistry)
(Anti-GRP94 antibody, AAA45169, IHC(P)IHC(P): HELA Cell)
WB (Western Blot)
(Anti-GRP94 antibody, AAA45169, Western blottingLane 1: RAJI Cell LysateLane 2: MCF-7 Cell Lysate)
WB (Western Blot)
(Anti-GRP94 antibody, AAA45169, Western blottingLane 1: RAJI Cell LysateLane 2: MCF-7 Cell Lysate)
Related Product Information for anti-HSP90B1 antibody
Description: Rabbit IgG polyclonal antibody for Endoplasmin(HSP90B1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human, Mouse, Rat.
Background: Heat shock protein HSP 90-beta is a protein that in humans is encoded by the HSP90AB1 gene. Hsp90beta is mapped to chromosome 12q23.3. The function of Hsp90betaincludes assisting in protein folding, cell signaling, and tumor repression. This protein was first isolated by extracting proteins from stressed cells. These cells were stressed by heating, dehydrating or by other means, all of which caused the cell's proteins to begin to denature.
Background: Heat shock protein HSP 90-beta is a protein that in humans is encoded by the HSP90AB1 gene. Hsp90beta is mapped to chromosome 12q23.3. The function of Hsp90betaincludes assisting in protein folding, cell signaling, and tumor repression. This protein was first isolated by extracting proteins from stressed cells. These cells were stressed by heating, dehydrating or by other means, all of which caused the cell's proteins to begin to denature.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
92,469 Da
NCBI Official Full Name
Endoplasmin
NCBI Official Synonym Full Names
heat shock protein 90kDa beta (Grp94), member 1
NCBI Official Symbol
HSP90B1
NCBI Official Synonym Symbols
ECGP; GP96; TRA1; GRP94; HEL35; HEL-S-125m
NCBI Protein Information
endoplasmin; tumor rejection antigen 1; epididymis luminal protein 35; 94 kDa glucose-regulated protein; tumor rejection antigen (gp96) 1; endothelial cell (HBMEC) glycoprotein; heat shock protein 90 kDa beta member 1; stress-inducible tumor rejection antigen gp96; epididymis secretory sperm binding protein Li 125m
UniProt Protein Name
Endoplasmin
UniProt Gene Name
HSP90B1
UniProt Synonym Gene Names
GRP94; TRA1; GRP-94
UniProt Entry Name
ENPL_HUMAN
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Product Notes
The HSP90B1 hsp90b1 (Catalog #AAA45169) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-GRP94 antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's GRP94 can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IHC (Immunohistochemistry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the HSP90B1 hsp90b1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GRP94, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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