Mouse anti-Human, Mouse GSK3 beta Monoclonal Antibody | anti-GSK3B antibody

Anti-GSK3 beta Antibody

Cat. Num. AAA290858

• Reactivity: Human, Mouse
• Applications: Immunoprecipitation, Immunofluorescence, Western Blot
• Purity: Affinity purified.
• Synonyms: GSK3 beta, Antibody; Anti-GSK3 beta Antibody; Glycogen Synthase Kinase 3 Beta; Glycogen synthase kinase-3 beta; GSK 3 beta; GSK-3 beta; GSK3B; GSK3B_HUMAN; GSK3beta isoform; Serine/threonine-protein kinase GSK3B.; anti-GSK3B antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Purity/Purification: Affinity purified.
• Form/Format: PBS (pH 7.4) containing with 0.03% Proclin300 and 50% glycerol.
• Sequence Length: 420

• Applicable Applications for anti-GSK3B antibody: IP (Immunoprecipitation), IF (Immunofluorescence), WB (Western Blot)
• Immunogen: Purified recombinat GSK-3Beta Protein fragments expressed in E.coli.
• Function: Constitutively active protein kinase that acts as a negative regulator in the hormonal control of glucose homeostasis, Wnt gnalingand regulation of transcription factors and icrotubules, by phosphorylating and inactivating glycogen synthase (GYS1 or GYS2), EIF2B, CTNNB1/beta-catenin, APC AXIN1, DPYSL2/CRMP2, JUN, NFATC1/NFATC, MAPT/TAU and MACF1. Requires primed phosphorylation of the majority of its substrates. In skeletal muscle, contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. May also mediate the development of insulin resistance by regulating activation of transcription factors. Regulates protein synthesis by controlling the activity of initiation factor 2B (EIF2BE/EIF2B5) in the same manner as glycogen synthase. In Wnt signaling, GSK3B forms a multimeric complex with APC, AXIN1 and CTNNB1/beta-cateninand phosphorylates the N-terminus of CTNNB1 leading to its degradation mediated by ubiquitin/proteasomes. Phosphorylates JUNat sites proximal to its DNA-binding domain,thereby reducing its affinity for DNA.Phosphorylates NFATC1/NFATC on conservedserine residues promoting NFATC1/NFATCnuclear export, shutting off NFATC1/NFATCgene regulation, and thereby opposing theaction of calcineurin. Phosphorylates MAPT/TAU on 'Thr-548', decreasing significantly MAPT/TAU ability to bind and stabilize microtubules. MAPT/TAU is the principal component of neurofibrillary tangles in Alzheimer disease. Plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex.Phosphorylates MACF1, inhibiting its binding to microtubules which is critical for its role in bulge stem cell migration and skin wound repair. Probably regulates NF-kappa-B (NFKB1) at the transcriptional level and is required for the NF-kappa-B-mediated anti-apoptotic response to TNF-alpha (TNF/TNFA). Negatively regulates replicationin pancreatic beta-cells, resulting in apoptosis, loss of beta-cells and diabetes.hrough phosphorylation of the anti-apoptotic protein MCL1, may control cell apoptosis in response to growth factors deprivation. Phosphorylates MUC1 in breast cancer cells,decreasing the interaction of MUC1 with CTNNB1/beta-catenin. Is necessary for the stablishment of neuronal polarity and axon growth. hosphorylates MARK2, leading to inhibit its activity. Phosphorylates SIK1 at'Thr-182', leading to sustain its activity.Phosphorylates ZC3HAV1 which enhances its antiviral activity. Phosphorylates SNAI1,leading to its BTRC-triggered ubiquitinationand proteasomal degradation. Phosphorylats SFPQ at 'Thr-687' upon T-cell activation.Phosphorylates NR1D1 st 'Ser-55' and 'Ser-59' and stabilizes it by protecting it from proteasomal degradation. Regulates thecircadian clock via phosphorylation of the major clock components including ARNTL/BMAL1, CLOCK and PER2.Phosphorylates CLOCK AT 'Ser-427' and targets it for proteasomal degradation.Phosphorylates ARNTL/BMAL1 at 'Ser-17' and 'Ser-21' and primes it for ubiquitination and proteasomal degradation. PhosphorylatesOGT at 'Ser-3' or 'Ser-4' which positively regulates its activity. Phosphorylates MYCN in neuroblastoma cells which may promote its used for the western blot analysi (1:1000 diluted). Anti-GSK3 ? Antibody - Background Participates in the Wnt signaling pathway.Implicated in the hormonal control of several regulatory proteins including glycogen syntase,MYB and the transcription factor JUN Phosphorylates JUN at sites proximal to itsDNA-binding domain, thereby reducindegradation (PubMed: ).
• Preparation and Storage: Store at -20°C. Stable for 12 months from date of receipt

IP (Immunoprecipitation)

(Immunoprecipitation analysis of Hela cell lysates using GSK-3beta.GSK-3beta Rabbit mAb was used for the western blot analysis (1:1000 diluted).)

ICC (Immunocytochemistry)

(Immunocytochemistry staining of HeLa cells fixed with -20 degree C Methanol and using GSK-3beta mouse mAb (dilution 1:200).)

WB (Western Blot)

(Western blot analysis of extracts from A549, Hela, 3T3 and Rat Brain cell lysates using GSK-3beta mouse mAb (1:1000 diluted).Predicted band size:46KDa.Observed band size:46KDa.)

NCBI and Uniprot Product Information

• NCBI GI #: 225903437
• NCBI GeneID: 2932
• NCBI Accession #: NP_001139628.1
• NCBI GenBank Nucleotide #: NM_002093
• UniProt Accession #: P49841; Q9BWH3; Q9UL47; D3DN89
• Molecular Weight: 46 KDa
• NCBI Official Full Name: glycogen synthase kinase-3 beta isoform 2
• NCBI Official Synonym Full Names: glycogen synthase kinase 3 beta
• NCBI Official Symbol: GSK3B
• NCBI Protein Information: glycogen synthase kinase-3 beta
• UniProt Protein Name: Glycogen synthase kinase-3 beta
• UniProt Gene Name: GSK3B
• UniProt Synonym Gene Names: GSK-3 beta