WB (Western Blot)
(Cobalt Chloride treated HeLa cells were subjected to SDS PAGE followed by western blot with AAA249054 (HIF1a Antibody) at dilution of 1:5000)
Mouse anti-Human HIF1a Monoclonal Antibody | anti-HIF1a antibody
Anti-HIF1a Antibody
Gene Names
HIF1A; HIF1; MOP1; PASD8; HIF-1A; bHLHe78; HIF-1alpha; HIF1-ALPHA; HIF-1-alpha
Reactivity
Human
Applications
Western Blot, Immunofluorescence, ELISA
Purity
>=95% as determined by SDS-PAGEProtein A+G Purified
Synonyms
HIF1a, Antibody; Anti-HIF1a Antibody; BHLHE78; MOP1; PASD8; anti-HIF1a antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Purity/Purification
>=95% as determined by SDS-PAGE
Protein A+G Purified
Protein A+G Purified
Form/Format
Liquid; PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Sequence Length
826
Applicable Applications for anti-HIF1a antibody
WB (Western Blot), IF (Immunofluorescence), ELISA
Immunogen
Hypoxia inducible factor 1, alpha subunit(basic helix-loop-helix transcription factor)
Preparation and Storage
Store at -20 degree C for 24 months. Avoid repeated freeze/thaw cycles.
WB (Western Blot)
(Cobalt Chloride treated HeLa cells were subjected to SDS PAGE followed by western blot with AAA249054 (HIF1a Antibody) at dilution of 1:5000)
WB (Western Blot)
(Cobalt Chloride treated HeLa cells were subjected to SDS PAGE followed by western blot with AAA249054 (HIF1a Antibody) at dilution of 1:5000)
IF (Immunofluorescence)
(Immunofluorescent analysis of (-20°C Ethanol) fixed Cobalt Chloride treated HeLa cells using AAA249054 (HIF1a antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG (H+L))
IF (Immunofluorescence)
(Immunofluorescent analysis of (-20°C Ethanol) fixed Cobalt Chloride treated HeLa cells using AAA249054 (HIF1a antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG (H+L))
Related Product Information for anti-HIF1a antibody
Under normal oxygen conditions, HIF1a is continuously produced and destroyed, in a process involving hydroxylation, interaction with von Hippel-Lindau (VHL) protein, polyubiquitylation and subsequent proteasomal degradation. Under hypoxic conditions, hydroxylation is impaired and HIF1a is stabilized. HIF1a localizes in cytoplasm in normoxia, but it can translocate into nuclear in response to hypoxia. The calculated molecular weight of HIF1a is 93 kDa, but the modified protein HIF1a is about 110-120kDa (PMID: 11698256,.PMID: 7539918).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
120 kDa
NCBI Official Full Name
hypoxia-inducible factor 1-alpha isoform 1
NCBI Official Synonym Full Names
hypoxia inducible factor 1 subunit alpha
NCBI Official Symbol
HIF1A
NCBI Official Synonym Symbols
HIF1; MOP1; PASD8; HIF-1A; bHLHe78; HIF-1alpha; HIF1-ALPHA; HIF-1-alpha
NCBI Protein Information
hypoxia-inducible factor 1-alpha
UniProt Protein Name
Hypoxia-inducible factor 1-alpha
UniProt Gene Name
HIF1A
UniProt Synonym Gene Names
BHLHE78; MOP1; PASD8; HIF-1-alpha; HIF1-alpha; bHLHe78
UniProt Entry Name
HIF1A_HUMAN
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Product Notes
The HIF1a hif1a (Catalog #AAA249054) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-HIF1a Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HIF1a can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IF (Immunofluorescence), ELISA. Researchers should empirically determine the suitability of the HIF1a hif1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HIF1a, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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