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IF (Immunofluorescence)
(Immunofluorescent analysis of Phosphorylation of H2A.X at Serine 139 in A549(upper.untreated or Hydroxyurea-treated) and Hela(lower.untreated or Hydroxyurea-treated) using Phospho-Histone H2A.X (Ser139) mouse mAb (1:400).)
Mouse anti-Human, Mouse Histone H2A.X Monoclonal Antibody | anti-H2A.X antibody
Histone H2A.X (Phospho-Ser139) Mouse mAb
Gene Names
H2AFX; H2AX; H2A.X; H2A/X
Reactivity
Human, Mouse
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Synonyms
Histone H2A.X, Antibody; Histone H2A.X (Phospho-Ser139) Mouse mAb; Histone H2A.x; H2AX; H2A histone family, member X; H2A.X; H2a/x; H2AFX; H2AX histone; H2AX_HUMAN; Histone H2A.X; Histone H2AX; anti-H2A.X antibody
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Specificity
This antibody detects endogenous levels of H2A.X only when phosphorylated at serine 139.
Purity/Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Form/Format
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-H2A.X antibody
ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot)
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding Ser139 of human H2A.X.
Modification
Phospho Specific
Preparation and Storage
Store at -20 degree C/1 year
IF (Immunofluorescence)
(Immunofluorescent analysis of Phosphorylation of H2A.X at Serine 139 in A549(upper.untreated or Hydroxyurea-treated) and Hela(lower.untreated or Hydroxyurea-treated) using Phospho-Histone H2A.X (Ser139) mouse mAb (1:400).)
IF (Immunofluorescence)
(Immunofluorescent analysis of Phosphorylation of H2A.X at Serine 139 in A549(upper.untreated or Hydroxyurea-treated) and Hela(lower.untreated or Hydroxyurea-treated) using Phospho-Histone H2A.X (Ser139) mouse mAb (1:400).)
IF (Immunofluorescence)
(Immunofluorescent analysis of Phosphorylation of H2A.X at Serine 139 in 3T3 or Hydroxyurea-treated 3T3 cells using Phospho-Histone H2A.X)
IF (Immunofluorescence)
(Immunofluorescent analysis of Phosphorylation of H2A.X at Serine 139 in 3T3 or Hydroxyurea-treated 3T3 cells using Phospho-Histone H2A.X)
WB (Western Blot)
(Western blot detection of Phosphorylation of H2A.X at Serine 139 in 3T3 or Hydroxyurea-treated 3T3 cell lysates using Phospho-Histone H2A.X (Ser139) mouse mAb (1:2000 diluted).Predicted band size:15KDa.Observed band size:15KDa.)
WB (Western Blot)
(Western blot detection of Phosphorylation of H2A.X at Serine 139 in 3T3 or Hydroxyurea-treated 3T3 cell lysates using Phospho-Histone H2A.X (Ser139) mouse mAb (1:2000 diluted).Predicted band size:15KDa.Observed band size:15KDa.)
WB (Western Blot)
(Western blot analysis of extracts from untreated or Hydroxyurea-treated Hela and A549 cells. using Histone H2A.X(Phospho-Ser139) mouse mAb (1:1000 diluted)(upper) or ?-Actin Mouse mAb (200068-8F10)(lower).Predicted band size:15KDa.Observed band size:15KDa.)
WB (Western Blot)
(Western blot analysis of extracts from untreated or Hydroxyurea-treated Hela and A549 cells. using Histone H2A.X(Phospho-Ser139) mouse mAb (1:1000 diluted)(upper) or ?-Actin Mouse mAb (200068-8F10)(lower).Predicted band size:15KDa.Observed band size:15KDa.)
Related Product Information for anti-H2A.X antibody
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures.This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq, Oct 2015]
Product Categories/Family for anti-H2A.X antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
15,145 Da
NCBI Official Full Name
histone H2A.x
NCBI Official Synonym Full Names
H2A histone family, member X
NCBI Official Symbol
H2AFX
NCBI Official Synonym Symbols
H2AX; H2A.X; H2A/X
NCBI Protein Information
histone H2A.x; H2AX histone
UniProt Protein Name
Histone H2A.x
UniProt Gene Name
H2AFX
UniProt Synonym Gene Names
H2AX; H2a/x
UniProt Entry Name
H2AX_HUMAN
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Product Notes
The H2A.X h2afx (Catalog #AAA320396) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Histone H2A.X (Phospho-Ser139) Mouse mAb reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H2A.X can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the H2A.X h2afx for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H2A.X, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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