WB (Western Blot)
(Western blot analysis of HeLa cell nuclear extract (20 ug) probed with histone H3 (pSer10) antibody, Clone 6G8B7 (AAA50439) at a 2 ug/ml dilution.)
Rat anti-Human Histone H3 Monoclonal Antibody | anti-HIST1H3F antibody
Histone H3 (pSer10) antibody | 6G8B7
Gene Names
HIST1H3A; H3/A; H3FA
Reactivity
Human
Applications
Western Blot, Immunofluorescence, ELISA
Purity
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Synonyms
Histone H3, Antibody; Histone H3 (pSer10) antibody | 6G8B7; RAT ANTI HUMAN HISTONE H3 (pSer10); anti-HIST1H3F antibody
Host
Rat
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
6G8B7
Purity/Purification
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Form/Format
Purified IgG - Liquid
Phosphate buffered saline
0.035% Sodium Azide (NaN3), 30% Glycerol
Phosphate buffered saline
0.035% Sodium Azide (NaN3), 30% Glycerol
Concentration
IgG concentration 1.0mg/ml (varies by lot)
Applicable Applications for anti-HIST1H3F antibody
WB (Western Blot), IF (Immunofluorescence), ELISA
Immunogen
A peptide corresponding to amino acids 1-19 including phosphorylated serine 10 of human histone H3
Preparation and Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20 degree C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8 degree C for short term use (up to 4 weeks) and store the remaining aliquots at -20 degree C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
WB (Western Blot)
(Western blot analysis of HeLa cell nuclear extract (20 ug) probed with histone H3 (pSer10) antibody, Clone 6G8B7 (AAA50439) at a 2 ug/ml dilution.)
WB (Western Blot)
(Western blot analysis of HeLa cell nuclear extract (20 ug) probed with histone H3 (pSer10) antibody, Clone 6G8B7 (AAA50439) at a 2 ug/ml dilution.)
IF (Immunofluorescence)
(Immunofluorescence staining of HeLa cells probed with histone H3 (pSer10) antibody (AAA50439; top) and stained with Hoechst (bottom).)
IF (Immunofluorescence)
(Immunofluorescence staining of HeLa cells probed with histone H3 (pSer10) antibody (AAA50439; top) and stained with Hoechst (bottom).)
Related Product Information for anti-HIST1H3F antibody
Rat anti Human histone H3 (pSer10) antibody, clone 6G8B7 recognizes histone H3 when phosphorylated at serine 10.
Histone H3 is one of the four core histones that make up the nucleosome core particle. Nucleosomes are the smallest subunit of chromatin and are made up of 146 bp of DNA wrapped around an octamer comprised of pairs of the four core histones (H2A, H2B, H3, and H4) (Smith, 1991). Histones may be modified in several ways including acetylation, methylation, phosphorylation, ubiquitination, glycosylation, and SUMOylation (Koch et al. 2007). Phosphorylation of histone H3 has been shown to occur during the early stages of mitosis when chromosomes begin to condense, and during premature condensing of chromosomes during S-phase of the cell cycle. Phosphorylation of histone H3 at serine 10 has been linked to both transcription and cell division (Hans and Dimitrov, 2001).
Broad species cross-reactivity is expected from Rat anti Human histone H3 (pSer10) antibody based on sequence.
Histone H3 is one of the four core histones that make up the nucleosome core particle. Nucleosomes are the smallest subunit of chromatin and are made up of 146 bp of DNA wrapped around an octamer comprised of pairs of the four core histones (H2A, H2B, H3, and H4) (Smith, 1991). Histones may be modified in several ways including acetylation, methylation, phosphorylation, ubiquitination, glycosylation, and SUMOylation (Koch et al. 2007). Phosphorylation of histone H3 has been shown to occur during the early stages of mitosis when chromosomes begin to condense, and during premature condensing of chromosomes during S-phase of the cell cycle. Phosphorylation of histone H3 at serine 10 has been linked to both transcription and cell division (Hans and Dimitrov, 2001).
Broad species cross-reactivity is expected from Rat anti Human histone H3 (pSer10) antibody based on sequence.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
15,404 Da
NCBI Official Full Name
histone H3.1
NCBI Official Synonym Full Names
histone cluster 1, H3a
NCBI Official Symbol
HIST1H3A
NCBI Official Synonym Symbols
H3/A; H3FA
NCBI Protein Information
histone H3.1; histone H3/a; histone 1, H3a; H3 histone family, member A
UniProt Protein Name
Histone H3.1
UniProt Gene Name
HIST1H3A
UniProt Synonym Gene Names
H3FA
UniProt Entry Name
H31_HUMAN
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Product Notes
The HIST1H3F hist1h3a (Catalog #AAA50439) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Histone H3 (pSer10) antibody | 6G8B7 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H3 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IF (Immunofluorescence), ELISA. Researchers should empirically determine the suitability of the HIST1H3F hist1h3a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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