Mouse HLA ABC Monoclonal Antibody | anti-HLA ABC antibody

MOUSE ANTI HUMAN HLA ABC:HRP

Cat. Num. AAA11895

• Reactivity: Macaque, Bovine, Cynomolgus Monkey, Baboon, Rhesus Monkey, Chimpanzee, Gorilla, Shrew
• Applications: Immunohistochemistry
• Synonyms: HLA ABC, Antibody; MOUSE ANTI HUMAN HLA ABC:HRP; anti-HLA ABC antibody

• Host: Mouse
• Reactivity: Macaque, Bovine, Cynomolgus Monkey, Baboon, Rhesus Monkey, Chimpanzee, Gorilla, Shrew
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: W6/32
• Form/Format: Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
• Concentration: IgG concentration 1.0 mg/ml (varies by lot)

• Applicable Applications for anti-HLA ABC antibody: Immunohistology Frozen*
• Application Notes: Immunohistology - Frozen Application Note:; The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. We recommends the use of acetone fixation for frozen sections.
• Perservative Stabilisers: 0.01% Thiomersal
• Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
• Immunogen: Purified human tonsil lymphocyte membranes.
• Histology Positive Control Tissue: Tonsil
• Fusion Partners: Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1/1-Ag4.1 myeloma cell line.
• Buffer Solution: Phosphate buffered saline
• Target Species: Human
• Preparation and Storage: It is recommended to aliquot and store at -20 degree C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8 degree C for short term use (up to 4 weeks) and store the remaining aliquots at -20 degree C. Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended

Application Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human HLA ABC:Alexa Fluor 647)

Application Data

(Staining of human peripheral blood monocytes with Mouse anti Human HLA ABC:Biotin)

Application Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human HLA ABC)

Application Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human HLA ABC:Alexa Fluor 488)

Application Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human HLA ABC:FITC)

Application Data

(Staining of peripheral blood lymphocytes with Mouse anti Human HLA-ABC: Low Endotoxin)

Related Product Information for anti-HLA ABC antibody

Mouse anti Human HLA ABC antibody, clone W6/32 recognizes an antigenic determinant shared among products of the HLA A, B and C loci. Clone W6/32 recognizes a conformational epitope, reacting with HLA class I alpha3 and alpha2 domains. The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In humans, this complex is referred to as the human leukocyte antigen (HLA) region. There are 3 major MHC class I proteins encoded by the HLA which are HLA A, HLA B and HLA C. These proteins are found on the surface of almost all nucleated somatic cells. Mouse anti Human HLA ABC antibody, clone W6/32 is routinely tested in flow cytometry on human peripheral blood lymphocytes.