Rabbit HMGB1/Hmg 1 Monoclonal Antibody | anti-HMGB1/Hmg 1 antibody

Anti-HMGB1/Hmg 1 Rabbit Monoclonal Antibody

Cat. Num. AAA46860

• Gene Names: HMGB1; HMG1; HMG3; HMG-1; SBP-1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunocytochemistry, Immunohistochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
• Purity: Affinity-chromatography
• Synonyms: HMGB1/Hmg 1, Antibody; Anti-HMGB1/Hmg 1 Rabbit Monoclonal Antibody; High mobility group protein B1; High mobility group protein 1; HMG-1; HMGB1; HMG1; anti-HMGB1/Hmg 1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Purity/Purification: Affinity-chromatography
• Form/Format: Liquid
• Sequence Length: 215

• Applicable Applications for anti-HMGB1/Hmg 1 antibody: WB (Western Blot), ICC (Immunocytochemistry), IHC (Immunohistochemistry), IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
• Immunogen: A synthesized peptide derived from human HMGB1
• Subcellular Localization: Nucleus. Chromosome. Cytoplasm. Secreted. Cell membrane; Peripheral membrane protein; Extracellular side. Endosome. Endoplasmic reticulum-Golgi intermediate compartment. In basal state predominantly nuclear. Shuttles between the cytoplasm and the nucleus (PubMed: 12231511, PubMed: 17114460). Translocates from the nucleus to the cytoplasm upon autophagy stimulation (PubMed: 20819940). Release from macrophages in the extracellular milieu requires the activation of NLRC4 or NLRP3 inflammasomes (By similarity). Passively released to the extracellular milieu from necrotic cells by diffusion, involving the fully reduced HGMB1 which subsequently gets oxidized (PubMed: 19811284). Also released from apoptic cells (PubMed: 16855214, PubMed: 18631454). Active secretion from a variety of immune and non-immune cells such as macrophages, monocytes, neutrophils, dendritic cells and natural killer cells in response to various stimuli such as LPS and cytokines involves a nonconventional secretory process via secretory lysosomes (PubMed: 12231511, PubMed: 14532127, PubMed: 15944249). Secreted by plasma cells in response to LPS (By similarity). Found on the surface of activated platelets (PubMed: 11154118).
• Tissue Specificity: Ubiquituous. Expressed in platelets (PubMed: 11154118).
• Contents: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Preparation and Storage: Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

IHC (Immunohiostchemistry)

(Immunohistochemical analysis of paraffin-embedded human kidney, using HMGB1 Antibody(AAA46860)HMGB1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-HMGB1 Antibody (AAA46860)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

WB (Western Blot)

(Western blot analysis of HMGB1 expression in (1)HeLa cell lysate; (2)Rat brain lysate (AAA46860).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HMGB1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for HMGB1)

Related Product Information for anti-HMGB1/Hmg 1 antibody

Multifunctional redox sensitive protein with various roles in different cellular compartments. In the nucleus is one of the major chromatin-associated non-histone proteins and acts as a DNA chaperone involved in replication, transcription, chromatin remodeling, V(D)J Recombinantion, DNA repair and genome stability. Proposed to be an universal biosensor for nucleic acids. Promotes host inflammatory response to sterile and infectious signals and is involved in the coordination and integration of innate and adaptive immune responses. In the cytoplasm functions as sensor and/or chaperone for immunogenic nucleic acids implicating the activation of TLR9-mediated immune responses, and mediates autophagy. Acts as danger associated molecular pattern (DAMP) molecule that amplifies immune responses during tissue injury. Released to the extracellular environment can bind DNA, nucleosomes, IL-1 beta, CXCL12, AGER isoform 2/sRAGE, lipopolysaccharide (LPS) and lipoteichoic acid (LTA), and activates cells through engagement of multiple surface receptors. In the extracellular compartment fully reduced HMGB1 (released by necrosis) acts as a chemokine, disulfide HMGB1 (actively secreted) as a cytokine, and sulfonyl HMGB1 (released from apoptotic cells) promotes immunological tolerance (PubMed: 23519706, PubMed: 23446148, PubMed: 23994764, PubMed: 25048472). Has proangiogdenic activity (By similarity). May be involved in platelet activation (By similarity). Binds to phosphatidylserine and phosphatidylethanolamide (By similarity). Bound to RAGE mediates signaling for neuronal outgrowth (By similarity). May play a role in accumulation of expanded polyglutamine (polyQ) proteins such as huntingtin (HTT) or TBP (PubMed: 23303669, PubMed: 25549101).

Product Categories/Family for anti-HMGB1/Hmg 1 antibody

Epigenetics and Nuclear Signaling; histones; hmgs; Epigenetics and Nuclear Signaling; Transcription; Domain Families; hmg box; ChIP'ing Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 926459555
• NCBI GeneID: 3146
• NCBI Accession #: NP_001300821.1
• NCBI GenBank Nucleotide #: NM_001313892.1
• UniProt Accession #: P09429; Q14321; Q5T7C3; Q6IBE1; A5D8W9
• Molecular Weight: 24894 MW
• NCBI Official Full Name: high mobility group protein B1
• NCBI Official Synonym Full Names: high mobility group box 1
• NCBI Official Symbol: HMGB1
• NCBI Official Synonym Symbols: HMG1; HMG3; HMG-1; SBP-1
• NCBI Protein Information: high mobility group protein B1
• UniProt Protein Name: High mobility group protein B1
• UniProt Gene Name: HMGB1
• UniProt Synonym Gene Names: HMG1; HMG-1