Standard Curve (Sample)
Human High Mobility Group Protein 1 Antibody (HMGB1) ELISA Kit | HMGB1 elisa kit
Human Anti-High Mobility Group Protein 1 Antibody (Anti-HMGB1)(Competitive EIA) ELISA Kit
Gene Names
HMGB1; HMG1; HMG3; SBP-1
Reactivity
Human
Applications
ELISA
Synonyms
High Mobility Group Protein 1 Antibody (HMGB1); N/A; Human Anti-High Mobility Group Protein 1 Antibody (Anti-HMGB1)(Competitive EIA) ELISA Kit; ELISA Kit for Anti-High Mobility Group Protein 1 Antibody (Anti-HMGB1); Anti-HMGB1/Anti-High Mobility Group Protein 1 Antibody; HMG1; HMG3; SBP1; Sulfoglucuronyl Carbohydrate Binding Protein; Amphoterin; High Mobility Group Box 1 Protein; HMGB1 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Anti-HMGB1. No significant cross-reactivity or interference between Anti-HMGB1 and analogues was observed.
Samples
Human serum, plasma and other biological fluids
Detection Range
3.12-200ng/mL
Sensitivity
< 1.17ng/mL
Applicable Applications for HMGB1 elisa kit
ELISA
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Anti-HMGB1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Anti-HMGB1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
Store at 4 degree C for 1 month; -20 degree C for long-term (within shelf life)
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for HMGB1 elisa kit
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with HMGB1. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate solution is added, those wells that contain Anti-HMGB1 will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Anti-HMGB1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Intended Uses: The kit is an enzyme immunoassay for in vitro quantitative measurement of Anti-HMGB1 in human serum, plasma and other biological fluids.
Intended Uses: The kit is an enzyme immunoassay for in vitro quantitative measurement of Anti-HMGB1 in human serum, plasma and other biological fluids.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
24,894 Da
NCBI Official Full Name
high mobility group protein B1
NCBI Official Synonym Full Names
high mobility group box 1
NCBI Official Symbol
HMGB1
NCBI Official Synonym Symbols
HMG1; HMG3; SBP-1
NCBI Protein Information
high mobility group protein B1; HMG-1; Amphoterin; high-mobility group box 1; high mobility group protein 1; Sulfoglucuronyl carbohydrate binding protein; high-mobility group (nonhistone chromosomal) protein 1
UniProt Protein Name
High mobility group protein B1
UniProt Gene Name
HMGB1
UniProt Synonym Gene Names
HMG1; HMG-1
UniProt Entry Name
HMGB1_HUMAN
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Product Notes
The Human HMGB1 hmgb1 (Catalog #AAA150850) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA150850 ELISA Kit recognizes Human HMGB1. AAA Biotech's High Mobility Group Protein 1 Antibody (HMGB1) can be used in a range of immunoassay formats including, but not limited to, ELISA. Researchers should empirically determine the suitability of the HMGB1 hmgb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "High Mobility Group Protein 1 Antibody (HMGB1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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