Mouse HSP90beta Monoclonal Antibody | anti-HSP90beta antibody

HSP90beta monoclonal antibody (K3701)

Cat. Num. AAA77025

• Gene Names: HSP90AB1; HSP84; HSPC2; HSPCB; D6S182; HSP90B
• Reactivity: Human, Mouse, Rat, Sheep, Dog, Pig, Bovine, Guinea pig, Hamster, Rabbit
• Applications: Western Blot, Flow Cytometry, Functional Assay
• Purity: Ammonium sulfate precipitation purified.
• Synonyms: HSP90beta, Antibody; HSP90beta monoclonal antibody (K3701); HSP84; Heat shock protein 90beta; HSP90beta, mAb (K3701); anti-HSP90beta antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat, Sheep, Dog, Pig, Bovine, Guinea pig, Hamster, Rabbit
• Clonality: Monoclonal
• Isotype: IgM
• Clone Number: K3701
• Specificity: Recognizes human, mouse, rat, bovine, dog, guinea pig, hamster, pig, rabbit and sheep HSP90beta. Detects a band of ~90kDa by Western blot.
• Purity/Purification: Ammonium sulfate precipitation purified.
• Form/Format: Liquid. In saline, pH 6.9, containing 0.1% sodium azide.
• Sequence Length: 724

• Applicable Applications for anti-HSP90beta antibody: WB (Western Blot), FCM/FACS (Flow Cytometry)
• Immunogen: Recombinant human HSP90beta.
• Preparation and Storage: Long Term: -20 degree C; Avoid freeze/thaw cycles.

FCM/FACS (Flow Cytometry)

(Flow cytometric analysis human colon cancer Caco-2 cells analyzed by flow cytometry using isotype control antibody (left) or Hsp90 beta mAb (K3701) (right).)

WB (Western Blot)

(Western blot analysis: Lane 1: MW marker, Lane 2: HeL a, Lane 3: PC-12, Lane 4: CHO-K1, Lane 5: HSP90 Native Protein, Lane 6: HSP90 alpha Recombinant Protein (negative control), probed with HSP90 beta mAb (K3701))

Related Product Information for anti-HSP90beta antibody

The Hsp90 family of heat shock proteins represents one of the most abundantly expressed and highly conserved families of cellular chaperones whose expression can be upregulated under conditions of cellular stress, and includes cytoplasmic (Hsp90-alpha/beta), ER (grp94), and mitochondrial (TRAP1) localized members. Structurally, Hsp90 is characterized by an N-terminal ATP-binding domain, a medial substrate-binding domain, and a C-terminal dimerization motif. Hsp90 dimers function in cooperation with cochaperones (e.g. Hsp40, Hsp70, Hop, p23) to stabilize a multitude of client protein substrates, including steroid hormone receptors, protein kinases, and transcription factors. The essential binding and hydrolysis of ATP by Hsp90 is inhibited by ansamycin drugs (e.g. geldanamycin, 17-AAG) which occupy the N-terminal Hsp90 nucleotide-binding pocket. Many Hsp90 client proteins such as erbB2/Her-2, c-raf, bcr-abl, p53, and hTERT, are members of well characterized oncogenic pathways, making Hsp90 inhibitors useful anticancer agents.

Product Categories/Family for anti-HSP90beta antibody

Protein Synthesis & Folding; Proteolysis & Protein Degradation; Cell Death

NCBI and Uniprot Product Information

• NCBI GI #: 17865718
• NCBI GeneID: 3326
• UniProt Accession #: P08238; Q5T9W7; Q9NQW0; Q9NTK6; B2R5P0
• Molecular Weight: 83,264 Da
• NCBI Official Full Name: Heat shock protein HSP 90-beta
• NCBI Official Synonym Full Names: heat shock protein 90 alpha family class B member 1
• NCBI Official Symbol: HSP90AB1
• NCBI Official Synonym Symbols: HSP84; HSPC2; HSPCB; D6S182; HSP90B
• NCBI Protein Information: heat shock protein HSP 90-beta
• UniProt Protein Name: Heat shock protein HSP 90-beta
• UniProt Gene Name: HSP90AB1
• UniProt Synonym Gene Names: HSP90B; HSPC2; HSPCB; HSP 90; HSP 84; HSP84