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FCM (Flow Cytometry)
(Flow cytometric analysis of PC-3M cells with hUPF1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
Rabbit anti-Human, Mouse hUPF1 Monoclonal Antibody | anti-hUPF1 antibody
hUPF1 Antibody
Gene Names
UPF1; HUPF1; NORF1; RENT1; smg-2; pNORF1
Reactivity
Human, Mouse
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
ProA affinity purified
Synonyms
hUPF1; Monoclonal Antibody; hUPF1 Antibody; ATP dependent helicase RENT1 antibody; ATP-dependent helicase RENT1 antibody; Delta helicase antibody; FLJ43809 antibody; FLJ46894 antibody; HUPF 1 antibody; hUpf1 antibody; KIAA0221 antibody; Nonsense mRNA reducing factor 1 antibody; NORF 1 antibody; NORF1 antibody; pNORF 1 antibody; pNORF1 antibody; Regulator of nonsense transcripts 1 antibody; RENT 1 antibody; RENT1 antibody; RENT1_HUMAN antibody; Smg 2 antibody; Smg 2 homolog nonsense mediated mRNA decay factor antibody; UP Frameshift 1 antibody; Up frameshift mutation 1 homolog (S. cerevisiae) antibody; Up frameshift mutation 1 homolog antibody; Up frameshift suppressor 1 homolog antibody; Up-frameshift suppressor 1 homolog antibody; UPF 1 antibody; UPF 1 regulator of nonsense transcripts homolog antibody; upf1 antibody; UPF1 regulator of nonsense transcripts homolog antibody; UPF1 RNA helicase and ATPase antibody; Yeast Upf1p homolog antibody; anti-hUPF1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Monoclonal
Clone Number
JG38-44
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-hUPF1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
IP: 1:10-1:50
FC/FACS: 1:50-1:100
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
IP: 1:10-1:50
FC/FACS: 1:50-1:100
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein within human hUPF1 aa 1-200.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
FCM (Flow Cytometry)
(Flow cytometric analysis of PC-3M cells with hUPF1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
FCM (Flow Cytometry)
(Flow cytometric analysis of PC-3M cells with hUPF1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry)
(ICC staining hUPF1 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining hUPF1 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining hUPF1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining hUPF1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining hUPF1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining hUPF1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti- hUPF1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti- hUPF1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti- hUPF1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti- hUPF1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-hUPF1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-hUPF1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human colon tissue using anti- hUPF1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human colon tissue using anti- hUPF1 antibody. Counter stained with hematoxylin.)
WB (Western Blot)
(Western blot analysis of hUPF1 on PC-3M cell lysate using anti-hUPF1 antibody at 1/1, 000 dilution.)
WB (Western Blot)
(Western blot analysis of hUPF1 on PC-3M cell lysate using anti-hUPF1 antibody at 1/1, 000 dilution.)
Related Product Information for anti-hUPF1 antibody
In eukaryotes, it is essential to have the ability to detect and degrade transcripts that lack full coding potential. Nonsense-mediated RNA decay (NMD) protects the organism by avoiding the translation of truncated peptides with dominant negative or deleterious gain-of-function potential. Rent1, a mammalian ortholog of Upflp, is essential for embryonic viability. Rent1 (also designated regulator of nonsense transcripts and HUpf1) contains an N-terminal zinc finger-like domain, NTPase domains and a region comprised of domains that define Rent1 as a superfamily group I helicase. Rent1 protein has nucleic-acid-dependent ATPase activity and 5' to 3' helicase activity. In addition, Rent1 is an RNA-binding protein whose activity is modulated by ATP and directly interacts with Rent2, which is a mammalian homolog of Upf2p. Two mammalian orthologs to Upf3p, Rent3a and Rent3b, are encoded by two separate genes. Rent3b (also known as Rent3X) is encoded by a X-linked gene and localizes primarily to the nucleus, while Rent 1 and Rent 2 localize primarily in the cytoplasm. Specific Rent3 protein interactions with Y14 and spliced mRNA suggest Rent3a and Rent3b serve as a link between splicing and NMD in the cytoplasm.
Product Categories/Family for anti-hUPF1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
1129
NCBI Official Full Name
Regulator of nonsense transcripts 1
NCBI Official Synonym Full Names
UPF1 regulator of nonsense transcripts homolog (yeast)
NCBI Official Symbol
UPF1
NCBI Official Synonym Symbols
HUPF1; NORF1; RENT1; smg-2; pNORF1
NCBI Protein Information
regulator of nonsense transcripts 1; delta helicase; yeast Upf1p homolog; ATP-dependent helicase RENT1; nonsense mRNA reducing factor 1; up-frameshift mutation 1 homolog; up-frameshift suppressor 1 homolog; smg-2 homolog, nonsense mediated mRNA decay fact
UniProt Protein Name
Regulator of nonsense transcripts 1
UniProt Gene Name
UPF1
UniProt Synonym Gene Names
KIAA0221; RENT1; NORF1; hUpf1
UniProt Entry Name
RENT1_HUMAN
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Product Notes
The hUPF1 upf1 (Catalog #AAA30506) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The hUPF1 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's hUPF1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB: 1:500-1:2000 IHC: 1:50-1:200 ICC/IF: 1:50-1:200 IP: 1:10-1:50 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the hUPF1 upf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "hUPF1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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