WB (Western Blot)
(Detection of human IDO1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa cells treated with IFN-gamma (+) or mock treated (-). Antibody: Rabbit anti-IDO1 recombinant monoclonal antibody (AAA213543 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Vinculin .)
IDO1 recombinant antibody
Rabbit anti-IDO1 Recombinant Monoclonal Antibody [BLR040F]
Gene Names
IDO1; IDO; INDO; IDO-1
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Purified
Synonyms
IDO1, Recombinant Antibody; Rabbit anti-IDO1 Recombinant Monoclonal Antibody [BLR040F]; IDO; indoleamine-pyrrole 2,3-dioxygenase; indoleamine 2,3-dioxygenase 1; indole 2,3-dioxygenase; indolamine 2,3 dioxygenase; IDO-1; INDO; Indoleamine 2,3-dioxygenase 1; IDO1 recombinant antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Clone Number
BLR040F
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Concentration
400ug/ml (varies by lot)
Applicable Applications for IDO1 recombinant antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Residues 1-403 (full length)
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human IDO1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa cells treated with IFN-gamma (+) or mock treated (-). Antibody: Rabbit anti-IDO1 recombinant monoclonal antibody (AAA213543 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Vinculin .)
WB (Western Blot)
(Detection of human IDO1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa cells treated with IFN-gamma (+) or mock treated (-). Antibody: Rabbit anti-IDO1 recombinant monoclonal antibody (AAA213543 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Vinculin .)
IP (Immunoprecipitation)
(Detection of human IDO1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells treated with IFN-gamma. Antibodies: Rabbit anti-IDO1 recombinant monoclonal antibody (AAA213543 lot 1) used for IP at 15 ul per reaction. IDO1 was also immunoprecipitated by rabbit anti-IDO1 recombinant monoclonal . For blotting immunoprecipitated IDO1, AAA213543 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human IDO1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells treated with IFN-gamma. Antibodies: Rabbit anti-IDO1 recombinant monoclonal antibody (AAA213543 lot 1) used for IP at 15 ul per reaction. IDO1 was also immunoprecipitated by rabbit anti-IDO1 recombinant monoclonal . For blotting immunoprecipitated IDO1, AAA213543 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Related Product Information for IDO1 recombinant antibody
Indoleamine 2,3-dioxygenase (IDO1) is a heme enzyme that catalyzes the first and rate-limiting step in tryptophan catabolism to N-formyl-kynurenine. IDO1 acts on multiple tryptophan substrates including D-tryptophan, L-tryptophan, 5-hydroxy-tryptophan, tryptamine, and serotonin. It is thought to play a role in a variety of pathophysiological processes such as antimicrobial and antitumor defense, neuropathology, immunoregulation, and antioxidant activity. Through its expression in dendritic cells, monocytes, and macrophages this enzyme modulates T-cell behavior by its peri-cellular catabolization of the essential amino acid tryptophan [taken from NCBI Entrez Gene (Gene ID: 3620)].
Product Categories/Family for IDO1 recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
45,326 Da
NCBI Official Full Name
indoleamine 2,3-dioxygenase 1
NCBI Official Synonym Full Names
indoleamine 2,3-dioxygenase 1
NCBI Official Symbol
IDO1
NCBI Official Synonym Symbols
IDO; INDO; IDO-1
NCBI Protein Information
indoleamine 2,3-dioxygenase 1
UniProt Protein Name
Indoleamine 2,3-dioxygenase 1
UniProt Gene Name
IDO1
UniProt Synonym Gene Names
IDO; INDO; IDO-1
UniProt Entry Name
I23O1_HUMAN
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Product Notes
The IDO1 ido1 (Catalog #AAA213543) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-IDO1 Recombinant Monoclonal Antibody [BLR040F] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IDO1 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the IDO1 ido1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IDO1, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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