IgG1 Recombinant Antibody | IGHG1 recombinant antibody

Rabbit Anti-Mouse IgG1 Secondary Antibody, Rabbit MAb

Cat. Num. AAA259413

• Reactivity: Mouse
• Applications: ELISA
• Purity: Protein A
• Synonyms: IgG1, Recombinant Antibody; Rabbit Anti-Mouse IgG1 Secondary Antibody, Rabbit MAb; Anti-IgG1 Antibody; Anti-Igh-4 Antibody; Anti-Ighg1 Antibody; Anti-VH7183 Antibody; IGHG1 recombinant antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone Number: 020
• Specificity: This antibody recognizes mouse IgG1, but almost not reacts to Mouse IgG2a, IgG2b, IgG3, IgM.
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for IGHG1 recombinant antibody: ELISA
• Immunogen: Recombinant Mouse IgG1-Fc Protein ( P01868-1; Val98-Lys324,C102S).
• Preparation and Storage: This antibody can be stored at 2 degree C-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.; This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

WB (Western Blot)

(Anti-Carboxypeptidase A monoclonal antibody at 1:1000 dilutionLane A: Recombinant Human Carboxypeptidase A Protein 100ngSecondaryRabbit Anti-Mouse IgG1/HRP(#AAA259413-H) at 1/1000 dilution.Developed using the ECL technique. Performed under reducing conditions.)

IHC (Immunohistochemisry)

(Immunohistochemical analysis of PFA-fixed paraffin-embedded human prostate tissue sections, with KLK15 primary antibody (1:60) for 1 hours at 37 degree C. An HRP-conjugated Rabbit Anti-Mouse IgG1 Secondary Antibody(AAA259413-H)(1:1600) was used as the secondary antibody.)

IHC (Immunohiostchemistry)

(Immunohistochemical analysis of PFA-fixed paraffin-embedded human stomach carcinoma tissue sections, with Claudin18.1 primary antibody (1:200) for 1 hours at 37 degree C. An HRP-conjugated Rabbit Anti-Mouse IgG1 Secondary Antibody(AAA259413-H)(1:1600) was used as the secondary antibody.)

ELISA

(ELISA of mouse immunoglobulins shows Rabbit Anti-Mouse IgG1 Secondary Antibody (#AAA259413) reacts only to mouse IgG1. No cross reactivity with mouse IgG2a, IgG2b, IgG3, IgM. The plate was coated with 0.1ug/mL of different mouse immunoglobulins. 0.1ug/mL of Rabbit Anti-Mouse IgG1 Secondary Antibody (#AAA259413) was used as the primary antibody. An HRP conjugated anti-Rabbit IgG as the secondary antibody.)

Related Product Information for IGHG1 recombinant antibody

As a monomeric immunoglobulin that is predominately involved in the secondary antibody response and the only isotype that can pass through the human placenta, Immunoglobulin G (IgG) is synthesized and secreted by plasma B cells, and constitutes 75% of serum immunoglobulins in humans. IgG antibodies protect the body against the pathogens by agglutination and immobilization, complement activation, toxin neutralization, as well as antibody-dependent cell-mediated cytotoxicity (ADCC). IgG tetramer contains two heavy chains (5 kDa) and two light chains (25 kDa) linked by disulfide bonds, that is the two identical halves form the Y-like shape. IgG is digested by pepsin proteolysis into Fab fragment (antigen-binding fragment) and Fc fragment ("crystallizable" fragment). IgG1 is most abundant in serum among the four IgG subclasses (IgG1, 2, 3 and 4) and binds to Fc receptors (FcgammaR) on phagocytic cells with high affinity. Fc fragment is demonstrated to mediate phagocytosis, trigger inflammation, and target Ig to particular tissues. Protein G or Protein A on the surface of certain Staphylococcal and Streptococcal strains specifically binds with the Fc region of IgGs, and has numerous applications in biotechnology as a reagent for affinity purification. Recombinant IgG Fc Region is suggested to represent a potential anti-inflammatory drug for treatment of human autoimmune diseases.

References

Carosell. ED. e. al.. 1988. Cel. Immunol. 112. 262-70. Eckl. I. e. al.. 1990. Cli. Ex. Immunol. 81. 352-6. Lorriain. C. e. al.. 1995. . Bio. Chem. 270:8164-71. Gomez-Guerrer. C. e. al.. 2000. . Immunol. 164. 2092-101. Spragu. ER. e. al.. 2004. . Bio. Chem. 14. 14184-93. Anthon. RM. e. al.. 2008. Science. 320. 373-6.

NCBI and Uniprot Product Information

• NCBI GI #: 121040
• UniProt Accession #: P01868
• Molecular Weight: 43,387 Da
• NCBI Official Full Name: Ig gamma-1 chain C region secreted form
• UniProt Protein Name: Ig gamma-1 chain C region secreted form
• UniProt Gene Name: Ighg1
• UniProt Synonym Gene Names: Igh-4