IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Tonsil stained with IgM Monoclonal Antibody (DA4-4))
Mouse anti-Human IgM (Immunoglobulin Mu Heavy Chain) Monoclonal Antibody | anti-IGHM antibody
IgM (Immunoglobulin Mu Heavy Chain) (B-Cell Marker) Mouse Monoclonal Antibody
Gene Names
IGHM; MU; VH; AGM1
Reactivity
Human
Applications
Immunohistochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
Synonyms
IgM (Immunoglobulin Mu Heavy Chain), Antibody; IgM (Immunoglobulin Mu Heavy Chain) (B-Cell Marker) Mouse Monoclonal Antibody; AGM1; IGHM; Constant Region of Heavy Chain of IgM; Ig Mu Chain C Region; anti-IGHM antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
DA4-4; same as SA-DA4 or HB57
Specificity
Recognizes a protein of 75kDa, identified as mu heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), gamma (IgG), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This MAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Sequence Length
452
Applicable Applications for anti-IGHM antibody
IHC (Immunohistochemistry), IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
Cellular Localization
Cytoplasm, Cell Surface and Secreted
Immunogen
Heavy chain of human IgM
Hu-Chromosome Location
14q32.33
Positive Control
293T, Raji or hPBL cells. Tonsil or Spleen.
Preparation and Storage
Antibody with azide - store at 2 to 8°C.
Antibody without azide - store at -20 to -80°C.
Non-hazardous. No MSDS required.
Antibody without azide - store at -20 to -80°C.
Non-hazardous. No MSDS required.
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Tonsil stained with IgM Monoclonal Antibody (DA4-4))
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Tonsil stained with IgM Monoclonal Antibody (DA4-4))
References
Maruyama S, et. al. Activation of human B cells and inhibition of their terminal differentiation by monoclonal anti-mu antibodies. Journal of Immunology 1985; 135(1):192-9. Rudich SM, et. al. Human B cell activation. Evidence for diverse signals provided by various monoclonal anti-IgM antibodies. Journal of Experimental Medicine, 1985; 162(4):1236-55
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
50-75kDa
NCBI Official Full Name
Ig mu chain C region
NCBI Official Synonym Full Names
immunoglobulin heavy constant mu
NCBI Official Symbol
IGHM
NCBI Official Synonym Symbols
MU; VH; AGM1
UniProt Protein Name
Ig mu chain C region
UniProt Gene Name
IGHM
UniProt Entry Name
IGHM_HUMAN
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Product Notes
The IGHM ighm (Catalog #AAA62615) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The IgM (Immunoglobulin Mu Heavy Chain) (B-Cell Marker) Mouse Monoclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IgM (Immunoglobulin Mu Heavy Chain) can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IF (Immunofluorescence), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the IGHM ighm for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IgM (Immunoglobulin Mu Heavy Chain), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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