Rabbit anti-Human IgM Monoclonal Antibody | anti-IGHM antibody

Rabbit anti-Human IgM mAb

Cat. Num. AAA283048

• Reactivity: Human
• Applications: ELISA, Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence, Western Blot
• Purity: Affinity purification
• Synonyms: IgM, Antibody; Rabbit anti-Human IgM mAb; MU; VH; AGM1; Human IgM; anti-IGHM antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Purity/Purification: Affinity purification
• Form/Format: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
• Sequence: QDTAIRVFAIPPSFASIFLTKSTKLTCLVTDLTTYDSVTISWTRQNGEAVKTHTNISESHPNATFSAVGEASICEDDWNSGERFTCTVTHTDLPSPLKQTISRPKGVALHRPDVYLLPPAREQLNLRESATITCLVTGFSPADVFVQWMQRGQPLSPEKYVTSAPMPEPQAPGRYFAHSILTVSEEEWNTGETYTCVVAHEALPNRVTERTVDKSTGKPTLYNVSLVMSDTAGTCY

• Applicable Applications for anti-IGHM antibody: ELISA, FCM/FACS (Flow Cytometry), ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot)
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 218-453 of Human IgM(P01871).
• Preparation and Storage: Store at -20 degree C. Avoid freeze/thaw cycles.

FCM/FACS (Flow Cytometry)

(Flow cytometry: 1X10^6 Human PBMC were surface-stained with Rabbit IgG isotype control (AC042,2 ug/mL,left) or Rabbit anti-Human IgM mAb (AAA283048,2 ug/mL,right), followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb staining.)

FCM/FACS (Flow Cytometry)

(Flow cytometry: 1X10^6 Daudi cells were surface-stained with Rabbit IgG isotype control (AC042,2 ug/mL,left) or Rabbit anti-Human IgM mAb (AAA283048,2 ug/mL,right), followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb staining.)

FCM/FACS (Flow Cytometry)

(Flow cytometry: 1X10^6 Jurkat cells (negative control,left) and Daudi cells (right) were surface-stained with Rabbit anti-Human IgM mAb (AAA283048,2 ug/mL,orange line) or ABflo® 647 Rabbit IgG isotype control (A22070,5 ul/Test,blue line), followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).)

ICC (Immunocytochemistry)

(Confocal imaging of Daudi cells using Rabbit anti-Human IgM mAb (AAA283048,dilution 1:200),(Red).DAPI was used for nuclear staining(blue).Objective:40x.)

WB (Western Blot)

(Western blot analysis of lysates from Raji cells, using Rabbit anti-Human IgM mAb (AAA283048) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Negative control (NC): JurkatExposure time: 20s.)

Related Product Information for anti-IGHM antibody

Immunoglobulins (Ig) are the antigen recognition molecules of B cells. An Ig molecule is made up of 2 identical heavy chains and 2 identical light chains (see MIM 147200) joined by disulfide bonds so that each heavy chain is linked to a light chain and the 2 heavy chains are linked together. Each Ig heavy chain has an N-terminal variable (V) region containing the antigen-binding site and a C-terminal constant (C) region, encoded by an individual C region gene, that determines the isotype of the antibody and provides effector or signaling functions. The heavy chain V region is encoded by 1 each of 3 types of genes: V genes (see MIM 147070), joining (J) genes (see MIM 147010), and diversity (D) genes (see MIM 146910). The C region genes are clustered downstream of the V region genes within the heavy chain locus on chromosome 14. The IGHM gene encodes the C region of the mu heavy chain, which defines the IgM isotype. Naive B cells express the transmembrane forms of IgM and IgD (see IGHD; MIM 1471770) on their surface. During an antibody response, activated B cells can switch to the expression of individual downstream heavy chain C region genes by a process of somatic recombination known as isotype switching. In addition, secreted Ig forms that act as antibodies can be produced by alternative RNA processing of the heavy chain C region sequences. Although the membrane forms of all Ig isotypes are monomeric, secreted IgM forms pentamers, and occasionally hexamers, in plasma (summary by Janeway et al., 2005).

Product Categories/Family for anti-IGHM antibody

Immunology Inflammation; Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 193806374
• NCBI GeneID: 3507
• UniProt Accession #: P01871; P20769
• Molecular Weight: Calculated MW: 49kDa; Observed MW: 75kDa
• UniProt Protein Name: Ig mu chain C region
• UniProt Gene Name: IGHM
• UniProt Entry Name: IGHM_HUMAN