WB (Western Blot)
(The antibody when used at 1:5,000 dilution (0.2ug/ml) recognizes 5ng/lane recombinant human IL-6 (rhIL-6) on blot. Lane 1 and 2: rhIL-6 loaded at 5ng/lane.)
Mouse IL-6 Monoclonal Antibody | anti-IL-6 antibody
mAb anti-Human IL-6
Gene Names
IL6; HGF; HSF; BSF2; IL-6; IFNB2
Reactivity
Human. Other species not tested
Applications
Flow Cytometry, Functional Assay, Western Blot, ELISA
Purity
Protein G affinity purified
Synonyms
IL-6, Antibody; mAb anti-Human IL-6; Interleukin-6 (IL-6); Mouse monoclonal antibody to human interleukin-6 (IL-6); anti-IL-6 antibody
Host
Mouse
Reactivity
Human. Other species not tested
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
8E10
Specificity
Reactive with recombinant human Interleukin-6 (IL-6). Reactivity with natural antigen has not been evaluated.
Purity/Purification
Protein G affinity purified
Form/Format
Lyophilized from a solution in 0.01M PBS, pH 7.0
Sequence Length
212
Applicable Applications for anti-IL-6 antibody
FCM/FACS (Flow Cytometry), WB (Western Blot), ELISA
Target Protein
Human IL-6
Immunogen
Purified recombinant human IL-6
Myeloma
F0
Light Chain
Kappa
Reconstitution
Double distillated water is recommended to reconstitute the antibody.
Fusion Myeloma
F0
Research Area
Cytokine, growth factor, inflammatory and autoimmune diseases
Preparation and Storage
Store at -20 degree C
WB (Western Blot)
(The antibody when used at 1:5,000 dilution (0.2ug/ml) recognizes 5ng/lane recombinant human IL-6 (rhIL-6) on blot. Lane 1 and 2: rhIL-6 loaded at 5ng/lane.)
WB (Western Blot)
(The antibody when used at 1:5,000 dilution (0.2ug/ml) recognizes 5ng/lane recombinant human IL-6 (rhIL-6) on blot. Lane 1 and 2: rhIL-6 loaded at 5ng/lane.)
Related Product Information for anti-IL-6 antibody
Description: Mouse monoclonal antibody to human interleukin-6 (IL-6)
background: IL-6, also called B-cell stimulatory factor-2 (BSF-2), is an important cytokine for the differentiation of B-cells into immunoglobulin-secreting cells. IL-6 is mainly produced by T-cells, macrophages and endothelial cells in response to pathogenic molecular stimuli during infection, trauma and the growth phase of some types of cancer. IL-6 is up-regulated by the binding of Toll-like receptors to antigen pattern ligands. In acute inflammatory phase, IL-6 causes increased body temperature by initiating synthesis of Prostaglandin E2 in hypothalamus.
background: IL-6, also called B-cell stimulatory factor-2 (BSF-2), is an important cytokine for the differentiation of B-cells into immunoglobulin-secreting cells. IL-6 is mainly produced by T-cells, macrophages and endothelial cells in response to pathogenic molecular stimuli during infection, trauma and the growth phase of some types of cancer. IL-6 is up-regulated by the binding of Toll-like receptors to antigen pattern ligands. In acute inflammatory phase, IL-6 causes increased body temperature by initiating synthesis of Prostaglandin E2 in hypothalamus.
Product Categories/Family for anti-IL-6 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
Interleukin-6
NCBI Official Synonym Full Names
interleukin 6
NCBI Official Symbol
IL6
NCBI Official Synonym Symbols
HGF; HSF; BSF2; IL-6; IFNB2
NCBI Protein Information
interleukin-6; B-cell differentiation factor; B-cell stimulatory factor 2; BSF-2; CDF; CTL differentiation factor; IFN-beta-2; hybridoma growth factor; interferon beta-2; interferon, beta 2; interleukin BSF-2
UniProt Protein Name
Interleukin-6
UniProt Gene Name
IL6
UniProt Entry Name
IL6_HUMAN
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Product Notes
The IL-6 il6 (Catalog #AAA77951) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The mAb anti-Human IL-6 reacts with Human. Other species not tested and may cross-react with other species as described in the data sheet. AAA Biotech's IL-6 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the IL-6 il6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL-6, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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