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Application Data
(SDS-PAGE analysis of GM-1E7 The antibody was purified by protein G affinity chromatography from cell culture supernatants and verified by SDS-Page (Fig.4). Fig.4: SDS-PAGE analysis of purified GM-1E7 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ug of purified GM-1E7 antibody. Proteins were separated by SDS-PAGE and stained with RAPID StainTM Reagent.)
Mouse anti-Human IL13R Monoclonal Antibody | anti-IL13RA1 antibody
anti-human IL13 receptor (IL13-R) monoclonal antibody
Gene Names
IL13RA1; NR4; CD213A1; IL-13Ra
Reactivity
Human
Applications
ELISA, ELISA, Flow Cytometry, Functional Assay
Purity
Protein G
Synonyms
IL13R, Antibody; anti-human IL13 receptor (IL13-R) monoclonal antibody; anti-IL13RA1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
GM-1E7
Specificity
anti-human IL13 receptor (IL13-R)
Purity/Purification
Protein G
Form/Format
Purified
Sequence Length
427
Applicable Applications for anti-IL13RA1 antibody
ELISA, ELISA, FCM/FACS (Flow Cytometry), AntagonisticAntibody
Buffer
phosphate buffered saline, pH 7.2
Immunogen
genetic immunisation with cDNA encoding human IL13-Ra1 (extracellular
domain)
Selection
based on recognition of the complete native protein expressed on transfected
mammalian cells
Immunogen
cDNA
Preparation and Storage
Short term: 2 degree C - 8 degree C; long term: -20 degree C (avoid repeated freezing and thawing)
Application Data
(SDS-PAGE analysis of GM-1E7 The antibody was purified by protein G affinity chromatography from cell culture supernatants and verified by SDS-Page (Fig.4). Fig.4: SDS-PAGE analysis of purified GM-1E7 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ug of purified GM-1E7 antibody. Proteins were separated by SDS-PAGE and stained with RAPID StainTM Reagent.)
Application Data
(SDS-PAGE analysis of GM-1E7 The antibody was purified by protein G affinity chromatography from cell culture supernatants and verified by SDS-Page (Fig.4). Fig.4: SDS-PAGE analysis of purified GM-1E7 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ug of purified GM-1E7 antibody. Proteins were separated by SDS-PAGE and stained with RAPID StainTM Reagent.)
Application Data
(Fig 3: Dose-dependent inhibition of cellular proliferation. Samples of TF-1 cells were incubated for 72 h with 10 nM human IL-13 (closed circles) or 10 nM human IL-4 (open circles) plus the indicated concentrations of purified GM- 1E7. Antibody GM1E7 blocks IL-13-dependent cell proliferation completely, but only marginally influences IL-4- activity on TF-1 cells.)
Application Data
(Fig 3: Dose-dependent inhibition of cellular proliferation. Samples of TF-1 cells were incubated for 72 h with 10 nM human IL-13 (closed circles) or 10 nM human IL-4 (open circles) plus the indicated concentrations of purified GM- 1E7. Antibody GM1E7 blocks IL-13-dependent cell proliferation completely, but only marginally influences IL-4- activity on TF-1 cells.)
Application Data
(Fig.2: Spectral Confocal Microscopy of CHO cells using GM-1E7 Cat.# AAA71460. CHO cells were transiently transfected with an expression vector encoding IL13-R. Binding of GM-1E7 was visualized with a FITC-conjugated secondary antibody (green). Actin filaments are labeled with Alexa Fluor-555 Phalloidin (red). Cell nuclei are stained with DAPI (blue).)
Application Data
(Fig.2: Spectral Confocal Microscopy of CHO cells using GM-1E7 Cat.# AAA71460. CHO cells were transiently transfected with an expression vector encoding IL13-R. Binding of GM-1E7 was visualized with a FITC-conjugated secondary antibody (green). Actin filaments are labeled with Alexa Fluor-555 Phalloidin (red). Cell nuclei are stained with DAPI (blue).)
Application Data
(Fig.1: FACS analysis of BOSC23 cells using GM-1E6 Cat.# AAA71460. BOSC23 cells were transiently transfected with an expression vector encoding either IL13-R (red curve) or an irrelevant protein (control transfectant: black curve). Binding of GM-1E7 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with IL13-R transfected cells.)
Application Data
(Fig.1: FACS analysis of BOSC23 cells using GM-1E6 Cat.# AAA71460. BOSC23 cells were transiently transfected with an expression vector encoding either IL13-R (red curve) or an irrelevant protein (control transfectant: black curve). Binding of GM-1E7 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with IL13-R transfected cells.)
Related Product Information for anti-IL13RA1 antibody
Description: Purified monoclonal mouse anitbody
Background: Interleukin 13 (IL-13) is a T cell derived cytokine involved in the regulation of inflammatory and immune responses. IL-13Ralpa1 together with IL-4Ralpa forms a functional receptor for both IL-4 and IL-13, which is why these two cytokines share many of their biological activities. The receptor is found on human B cells, monocytes and endothelial cells. However, no functional receptor is expressed on T cells, which explains why IL-13, in contrast to IL-4, fails to induce TH2-cell differentiation.
Background: Interleukin 13 (IL-13) is a T cell derived cytokine involved in the regulation of inflammatory and immune responses. IL-13Ralpa1 together with IL-4Ralpa forms a functional receptor for both IL-4 and IL-13, which is why these two cytokines share many of their biological activities. The receptor is found on human B cells, monocytes and endothelial cells. However, no functional receptor is expressed on T cells, which explains why IL-13, in contrast to IL-4, fails to induce TH2-cell differentiation.
References
Myrtek et al. (2004): Expression of interleukin-13 receptor alpha 1-subunit on peripheral blood eosinophils is regulated by cytokines. Immunology 112(4): 597-604.
Krause et al. (2006): Blockade of interleukin-13-mediated cell activation by a novel inhibitory antibody to human IL-13 receptor ?1. Mol Immunol 33: 1799-1807
Krause et al. (2006): Blockade of interleukin-13-mediated cell activation by a novel inhibitory antibody to human IL-13 receptor ?1. Mol Immunol 33: 1799-1807
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
interleukin-13 receptor subunit alpha-1
NCBI Official Synonym Full Names
interleukin 13 receptor, alpha 1
NCBI Official Symbol
IL13RA1
NCBI Official Synonym Symbols
NR4; CD213A1; IL-13Ra
NCBI Protein Information
interleukin-13 receptor subunit alpha-1; CT19; IL-13RA1; IL-13R-alpha-1; CD213a1 antigen; IL-13R subunit alpha-1; cancer/testis antigen 19; IL13 receptor alpha-1 chain; IL-13 receptor subunit alpha-1; bB128O4.2.1 (interleukin 13 receptor, alpha 1)
UniProt Gene Name
IL13RA1
UniProt Synonym Gene Names
IL13R; IL13RA; IL-13 receptor subunit alpha-1; IL-13R subunit alpha-1; IL-13R-alpha-1; IL-13RA1; CT19
UniProt Entry Name
I13R1_HUMAN
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Product Notes
The IL13RA1 il13ra1 (Catalog #AAA71460) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The anti-human IL13 receptor (IL13-R) monoclonal antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IL13R can be used in a range of immunoassay formats including, but not limited to, ELISA, ELISA, FCM/FACS (Flow Cytometry), AntagonisticAntibody. Researchers should empirically determine the suitability of the IL13RA1 il13ra1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL13R, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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