Mouse anti-Human IL23A / IL-23 p19 Monoclonal Antibody | anti-IL23A antibody

Mouse Monoclonal [clone HLT2736] (IgG1) to Human IL23A / IL-23 p19

Cat. Num. AAA51911

• Gene Names: IL23A; P19; SGRF; IL-23; IL-23A; IL23P19
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified
• Synonyms: IL23A / IL-23 p19, Antibody; Mouse Monoclonal [clone HLT2736] (IgG1) to Human IL23A / IL-23 p19; Anti-IL23A / IL-23 p19 Antibody (clone HLT2736) IHC-plus; IL23A; IL-23 subunit alpha; IL-23A; Interleukin 23 p19 subunit; Interleukin-23 subunit alpha; IL23P19; Interleukin-23 subunit p19; p19; SGRF; IL-23; IL-23 p19; IL-23-A; IL-23p19; Human IL23A; anti-IL23A antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: HLT2736
• Specificity: Human recombinant p40 and p19 with elastin linker
• Purity/Purification: Affinity Purified
• Form/Format: Phosphate-buffered solution, pH 7.2, 0.09% sodium azide. Sourced in TCS.
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 189

• Applicable Applications for anti-IL23A antibody: WB (Western Blot), IHC (Immunohistochemistry)
• Target Species: Human
• Immunogen Species: IL23A / IL-23 p19 antibody was raised against Human
• Antigen Type: Recombinant protein
• Immunogen: IL23A / IL-23 p19 antibody was raised against recombinant human IL23A / IL-23.
• Uses: IHC; IHC-Paraffin (10 ug/ml); Western Blot; (optimal dilution to be determined by the researcher)
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and AAA Biotech makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Store at 4°C. Store undiluted.

Related Product Information for anti-IL23A antibody

IL23 p19 (IL23A) is a subunit of the heterodimeric cytokine interleukin 23 (IL23). IL23 is composed of the IL23A subunit and the p40 subunit of interleukin 12 (IL12B). Both IL23 and IL12 can activate the transcription activator STAT4, and stimulate the production of interferon-gamma (IFNG). In contrast to IL12, which acts mainly on naïve CD4(+) T cells, IL23 preferentially acts on memory CD4(+) T cells.

Immunohistochemistry: MBS246319 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3708 was determined to be 10 ug/ml.

Product Categories/Family for anti-IL23A antibody

Family: Interleukin

NCBI and Uniprot Product Information

• NCBI GI #: 7706702
• NCBI GeneID: 51561
• NCBI Accession #: NP_057668.1
• NCBI GenBank Nucleotide #: NM_016584.2
• UniProt Accession #: Q9NPF7; Q6NZ80; Q6NZ82; Q9H2A5
• NCBI Official Full Name: interleukin-23 subunit alpha
• NCBI Official Synonym Full Names: interleukin 23, alpha subunit p19
• NCBI Official Symbol: IL23A
• NCBI Official Synonym Symbols: P19; SGRF; IL-23; IL-23A; IL23P19
• NCBI Protein Information: interleukin-23 subunit alpha; IL-23-A; IL-23p19; IL-23 subunit alpha; interleukin 23 p19 subunit; interleukin-23 subunit p19; JKA3 induced upon T-cell activation; interleukin-six, G-CSF related factor
• UniProt Protein Name: Interleukin-23 subunit alpha
• UniProt Gene Name: IL23A
• UniProt Synonym Gene Names: SGRF; IL-23 subunit alpha; IL-23-A; IL-23p19
• UniProt Entry Name: IL23A_HUMAN