Rabbit anti-Mouse IL2RG Monoclonal Antibody | anti-IL2RG antibody

Recombinant Anti-IL2RG Antibody, Rabbit Monoclonal

Cat. Num. AAA257084

• Reactivity: Mouse
• Applications: Immunofluorescence, Immunocytochemistry, Flow Cytometry, Functional Assay, ELISA
• Purity: Protein A
• Synonyms: IL2RG, Antibody; Recombinant Anti-IL2RG Antibody, Rabbit Monoclonal; CD132/IL2RG Antibody, Rabbit MAb; interleukin 2 receptor, gamma; Anti-CD132 Antibody; Anti-gamma(c) Antibody; Anti-gc Antibody; Anti-[g]c Antibody; anti-IL2RG antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone Number: 011
• Specificity: Mouse CD132/IL2RG
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-IL2RG antibody: IF (Immunofluorescence), ICC (Immunocytochemistry), FCM/FACS (Flow Cytometry), ELISA
• Antibody Type: Recombinant Antibody
• Immunogen: Recombinant Mouse CD132/IL2RG Protein
• Conjugation: Unconjugated
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Mouse CD132/IL2RG (rM CD132/IL2RG).
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

FCM/FACS (Flow Cytometry)

(Flow cytometric analysis of Mouse IL2RG(CD132) expression on BABL/c splenocytes. Cells were stained with purified anti-Mouse IL2RG(CD132), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)

IF (Immunofluorescence)

(Immunofluorescence staining of mouse IL2RG in mouse splenocytes. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-mouse IL2RG monoclonal antibody (1:60) at 4 degree C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green).)

Related Product Information for anti-IL2RG antibody

The common gamma chain (gammac) (or CD132), also known as interleukin-2 receptor subunit gamma or IL2RG, is a member of the type I cytokine receptor family expressed on most lymphocyte (white blood cell) populations, and its gene is found on the X-chromosome of mammals. The common gamma chain (gammac) (or IL2RG), is a cytokine receptor subunit that is common to the receptor complexes for at least six different interleukin receptors: IL-2, IL-4, IL-7, IL-9, IL-15, and the interleukin-21 receptor. It is a component of multiple cytokine receptors that are essential for lymphocyte development and function. X-linked severe combined immunodeficiency (X-SCID) is a rare and potentially fatal disease caused by mutations of IL2RG, the gene encoding IL2RG. IL2RG was demonstrated to be a component of the IL-4 receptor based on chemical cross-linking data, the ability of IL2RG to augment IL-4 binding affinity. The observation that IL-2R gamma is a functional component of the IL-4 receptor, together with the finding that IL-2R gamma associates with the IL-7 receptor, begins to elucidate why a deficiency of this common gamma chain (gamma c) has a profound effect on lymphoid function and development, as seen in X-linked severe combined immunodeficiency.

Product Categories/Family for anti-IL2RG antibody

Common G-Chain signaling pathway

References

Russell SM, et al. (1993) Interleukin-2 receptor gamma chain: a functional component of the interleukin-4 receptor. Science. 262 (5141): 1880-3.Miyazaki T, et al. (1994) Functional activation of Jak1 and Jak3 by selective association with IL-2 receptor subunits. Science. 266 (5187): 1045-7.Takeshita T, et al. (1992) Cloning of the gamma chain of the human IL-2 receptor. Science. 257 (5068): 379-82.

NCBI and Uniprot Product Information

• NCBI GI #: 7305181
• NCBI GeneID: 16186
• NCBI Accession #: NP_038591.1
• NCBI GenBank Nucleotide #: NM_013563.3
• UniProt Accession #: P34902
• Molecular Weight: 42,241 Da
• NCBI Official Full Name: cytokine receptor common subunit gamma
• UniProt Protein Name: Cytokine receptor common subunit gamma
• UniProt Gene Name: Il2rg
• UniProt Synonym Gene Names: IL-2 receptor subunit gamma; IL-2R subunit gamma; IL-2RG
• UniProt Entry Name: IL2RG_MOUSE