IF (Immunofluorescence)
(Figure 2. IF analysis of IL2 Receptor gamma/IL2RG using anti- IL2 Receptor gamma/IL2RG antibody (AAA125540).IL2 Receptor gamma/IL2RG was detected in immunocytochemical section of K562 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti- IL2 Receptor gamma/IL2RG Antibody (AAA125540) overnight at 4 degree C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
Rabbit IL2 Receptor gamma/IL2RG Polyclonal Antibody | anti-IL2RG antibody
Anti-IL2 Receptor gamma/IL2RG Antibody
Gene Names
IL2RG; P64; CIDX; IMD4; CD132; SCIDX; IL-2RG; SCIDX1
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Synonyms
IL2 Receptor gamma/IL2RG, Antibody; Anti-IL2 Receptor gamma/IL2RG Antibody; IL2RG; Cytokine receptor common subunit gamma; Interleukin-2 receptor subunit gamma; IL-2 receptor subunit gamma; IL-2R subunit gamma; IL-2RG; gammaC; p64; CD antigen CD132; interleukin 2 receptor subunit gamma; anti-IL2RG antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Rabbit IgG polyclonal antibody for IL2 Receptor gamma/IL2RG detection.
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized. Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Applicable Applications for anti-IL2RG antibody
IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot)
Immunogen
A synthetic peptide corresponding to a sequence of human IL2 Receptor gamma/IL2RG (QLQKKEIHLYQTFVVQLQD).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Recommended Detection Systems
Recommended Detection Systems
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
IF (Immunofluorescence)
(Figure 2. IF analysis of IL2 Receptor gamma/IL2RG using anti- IL2 Receptor gamma/IL2RG antibody (AAA125540).IL2 Receptor gamma/IL2RG was detected in immunocytochemical section of K562 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti- IL2 Receptor gamma/IL2RG Antibody (AAA125540) overnight at 4 degree C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
IF (Immunofluorescence)
(Figure 2. IF analysis of IL2 Receptor gamma/IL2RG using anti- IL2 Receptor gamma/IL2RG antibody (AAA125540).IL2 Receptor gamma/IL2RG was detected in immunocytochemical section of K562 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti- IL2 Receptor gamma/IL2RG Antibody (AAA125540) overnight at 4 degree C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
WB (Western Blot)
(Figure 1. Western blot analysis of IL2 Receptor gamma/IL2RG using anti-IL2 Receptor gamma/IL2RG antibody (AAA125540).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: human Jurkat whole cell lysatesLane 2: human K562 whole cell lysatesLane 3: human Raji whole cell lysatesLane 4: human U-937 whole cell lysatesLane 5: human HEK293 whole cell lysatesLane 6: human HMY2. CIR whole cell lysatesLane 7: human THP-1 whole cell lysatesLane 8: rat RH-35 whole cell lysatesLane 9: rat C6 whole cell lysatesLane 10: mouse HEPA1-6 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-IL2 Receptor gamma/IL2RG antigen affinity purified polyclonal antibody (Catalog # AAA125540) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IL2 Receptor gamma/IL2RG at approximately 65KD. The expected band size for IL2 Receptor gamma/IL2RG is at 65KD.)
WB (Western Blot)
(Figure 1. Western blot analysis of IL2 Receptor gamma/IL2RG using anti-IL2 Receptor gamma/IL2RG antibody (AAA125540).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: human Jurkat whole cell lysatesLane 2: human K562 whole cell lysatesLane 3: human Raji whole cell lysatesLane 4: human U-937 whole cell lysatesLane 5: human HEK293 whole cell lysatesLane 6: human HMY2. CIR whole cell lysatesLane 7: human THP-1 whole cell lysatesLane 8: rat RH-35 whole cell lysatesLane 9: rat C6 whole cell lysatesLane 10: mouse HEPA1-6 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-IL2 Receptor gamma/IL2RG antigen affinity purified polyclonal antibody (Catalog # AAA125540) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IL2 Receptor gamma/IL2RG at approximately 65KD. The expected band size for IL2 Receptor gamma/IL2RG is at 65KD.)
Related Product Information for anti-IL2RG antibody
IL2RG(INTERLEUKIN 2 RECEPTOR, GAMMA),also known as Common gamma chain or CD132, is a cytokine receptor sub-unit that is common to the receptor complexes for at least six different interleukin receptors:IL-2, IL-4, IL-7, IL-9, IL-15 and interleukin-21 receptor. IL2RG is a member of the type I cytokine receptor family expressed on most lymphocyte(white blood cell) populations, and its gene is found on the X-chromosome of mammals. By fluorescence in situ hybridization and PCR amplification of somatic cell hybrid DNAs, The IL2RG gene is mapped to Xq13. 1. The IL2RG gene contains 8 exons and spans approximately 4. 2 kb. And the gamma chain is an indispensable component of the functional IL2 receptor. Lymphocytes expressing the common gamma chain can form functional receptors for these cytokine proteins, which transmit signals from one cell to another and direct programs of cellular differentiation. The sharing of the gamma subunit by several receptors explains why humans and mice that lack IL2 entirely show milder symptoms than those with IL2RG deficiency.
References
1. Asao, H., Okuyama, C., Kumaki, S., Ishii, N., Tsuchiya, S., Foster, D., Sugamura, K. Cutting edge: the common gamma-chain is an indispensable subunit of the IL-21 receptor complex. J. Immun. 167: 1-5, 2001.
2. Brandt, K., Singh, P. B., Bulfone-Paus, S., Ruckert, R. Interleukin-21: a new modulator of immunity, infection, and cancer. Cytokine Growth Factor Rev. 18: 223-232, 2007.
3. PDB 2B5IWang X, Rickert M, Garcia KC (November 2005). "Structure of the quaternary complex of interleukin-2 with its alpha, beta, and gammac receptors". Science 310 (5751): 1159-63. doi:10. 1126/science. 1117893. PMID 16293754.
2. Brandt, K., Singh, P. B., Bulfone-Paus, S., Ruckert, R. Interleukin-21: a new modulator of immunity, infection, and cancer. Cytokine Growth Factor Rev. 18: 223-232, 2007.
3. PDB 2B5IWang X, Rickert M, Garcia KC (November 2005). "Structure of the quaternary complex of interleukin-2 with its alpha, beta, and gammac receptors". Science 310 (5751): 1159-63. doi:10. 1126/science. 1117893. PMID 16293754.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
369
NCBI Official Full Name
Cytokine receptor common subunit gamma
NCBI Official Synonym Full Names
interleukin 2 receptor, gamma
NCBI Official Symbol
IL2RG
NCBI Official Synonym Symbols
P64; CIDX; IMD4; CD132; SCIDX; IL-2RG; SCIDX1
NCBI Protein Information
cytokine receptor common subunit gamma; gammaC; CD132 antigen; IL-2R subunit gamma; IL-2 receptor subunit gamma; common cytokine receptor gamma chain
UniProt Protein Name
Cytokine receptor common subunit gamma
UniProt Gene Name
IL2RG
UniProt Synonym Gene Names
IL-2 receptor subunit gamma; IL-2R subunit gamma; IL-2RG
UniProt Entry Name
IL2RG_HUMAN
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Product Notes
The IL2RG il2rg (Catalog #AAA125540) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-IL2 Receptor gamma/IL2RG Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's IL2 Receptor gamma/IL2RG can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the IL2RG il2rg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL2 Receptor gamma/IL2RG, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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