WB (Western Blot)
(Western blot analysis of lysates from wild type(WT) and Integrin alpha 5 (ITGA5/CD49e) knockdown (KD) HeLa cells, using [KD Validated] Integrin alpha 5 (ITGA5/CD49e) Rabbit mAb (AAA282984) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 60s.)
Rabbit anti-Human Integrin alpha 5 (ITGA5/CD49e) Monoclonal Antibody | anti-ITGA5 antibody
[KD Validated] Integrin alpha 5 (ITGA5/CD49e) Rabbit mAb
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Affinity purification
Synonyms
Integrin alpha 5 (ITGA5/CD49e), Antibody; [KD Validated] Integrin alpha 5 (ITGA5/CD49e) Rabbit mAb; FNRA; CD49e; VLA-5; VLA5A; [KD Validated] Integrin alpha 5 (ITGA5/CD49e); anti-ITGA5 antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Sequence
FNLDAEAPAVLSGPPGSFFGFSVEFYRPGTDGVSVLVGAPKANTSQPGVLQGGAVYLCPWGASPTQCTPIEFDSKGSRLLESSLSSSEGEEPVEYKSLQWFGATVRAHGSSILACAPLYSWRTEKEPLSDPVGTCYLSTDNFTRILEYAPCRSDFSWAAGQGYCQGGFSAEFTKTGRVVLGGPGSYFWQGQILSATQEQIAESYYPEYLINLVQGQLQTRQASSIYDDSYLGYSVAVGEFSGDDTEDFVAGVPKGNLTYGYVTILNGSDIRSLYNFSGEQMASYFGYAVAATDVNGDGLDDLLVGAPLLMDRTPDGRPQEVGRVYVYLQHPAGIEPTPTLTLTGHDEFGRFGSSLTPLGDLDQDGYNDVAIGAPFGGETQQGVVFVFPGGPGGLGSKPSQVLQPLWAASHTPDFFGSALRGGRDLDGNGYPDLIVGSFGVDKAVVYRGRPIVSASASLTIFPAMFNPEERSCSLEGNPVACINLSFCLNASGKHVADSIGFTVELQLDWQKQKGGVRRALFLASRQATLTQTLLIQNGAREDCREMKIYLRNESEFRDKLSPIHIALNFSLDPQAPVDSHGLRPALHYQSKSRIEDKAQILLDCGEDNICVPDLQLEVFGEQNHVYLGDKNALNLTFHAQNVGEGGAYEAELRVTAPPEAEYSGLVRHPGNFSSLSCDYFAVNQSRLLVCDLGNPMKAGASLWGGLRFTVPHLRDTKKTIQFDFQILSKNLNNSQSDVVSFRLSVEAQAQVTLNGVSKPEAVLFPVSDWHPRDQPQKEEDLGPAVHHVYELINQGPSSISQGVLELSCPQALEGQQLLYVTRVTGLNCTTNHPINPKGLELDPEGSLHHQQKREAPSRSSASSGPQILKCPEAECFRLRCELGPLHQQESQSLQLHFRVWAKTFLQREHQPFSLQCEAVYKALKMPYRILPRQLPQKERQVATAVQWTKAEGSY
Applicable Applications for anti-ITGA5 antibody
ELISA, WB (Western Blot)
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 42-995 of human Integrin alpha 5 (ITGA5/CD49e). (NP_002196.4).
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
WB (Western Blot)
(Western blot analysis of lysates from wild type(WT) and Integrin alpha 5 (ITGA5/CD49e) knockdown (KD) HeLa cells, using [KD Validated] Integrin alpha 5 (ITGA5/CD49e) Rabbit mAb (AAA282984) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 60s.)
WB (Western Blot)
(Western blot analysis of lysates from wild type(WT) and Integrin alpha 5 (ITGA5/CD49e) knockdown (KD) HeLa cells, using [KD Validated] Integrin alpha 5 (ITGA5/CD49e) Rabbit mAb (AAA282984) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 60s.)
WB (Western Blot)
(Western blot analysis of lysates from A-549 cells, using [KD Validated] Integrin alpha 5 (ITGA5/CD49e) Rabbit mAb (AAA282984) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 60s.)
WB (Western Blot)
(Western blot analysis of lysates from A-549 cells, using [KD Validated] Integrin alpha 5 (ITGA5/CD49e) Rabbit mAb (AAA282984) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 60s.)
Related Product Information for anti-ITGA5 antibody
The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha subunit and a beta subunit that function in cell surface adhesion and signaling. The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha 5 subunit. This subunit associates with the beta 1 subunit to form a fibronectin receptor. This integrin may promote tumor invasion, and higher expression of this gene may be correlated with shorter survival time in lung cancer patients. Note that the integrin alpha 5 and integrin alpha V subunits are encoded by distinct genes.
Product Categories/Family for anti-ITGA5 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Calculated MW: 115kDa
Observed MW: 150kDa
Observed MW: 150kDa
UniProt Protein Name
Integrin alpha-5
UniProt Gene Name
ITGA5
UniProt Synonym Gene Names
FNRA
UniProt Entry Name
ITA5_HUMAN
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Product Notes
The ITGA5 itga5 (Catalog #AAA282984) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The [KD Validated] Integrin alpha 5 (ITGA5/CD49e) Rabbit mAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Integrin alpha 5 (ITGA5/CD49e) can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the ITGA5 itga5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: FNLDAEAPAV LSGPPGSFFG FSVEFYRPGT DGVSVLVGAP KANTSQPGVL QGGAVYLCPW GASPTQCTPI EFDSKGSRLL ESSLSSSEGE EPVEYKSLQW FGATVRAHGS SILACAPLYS WRTEKEPLSD PVGTCYLSTD NFTRILEYAP CRSDFSWAAG QGYCQGGFSA EFTKTGRVVL GGPGSYFWQG QILSATQEQI AESYYPEYLI NLVQGQLQTR QASSIYDDSY LGYSVAVGEF SGDDTEDFVA GVPKGNLTYG YVTILNGSDI RSLYNFSGEQ MASYFGYAVA ATDVNGDGLD DLLVGAPLLM DRTPDGRPQE VGRVYVYLQH PAGIEPTPTL TLTGHDEFGR FGSSLTPLGD LDQDGYNDVA IGAPFGGETQ QGVVFVFPGG PGGLGSKPSQ VLQPLWAASH TPDFFGSALR GGRDLDGNGY PDLIVGSFGV DKAVVYRGRP IVSASASLTI FPAMFNPEER SCSLEGNPVA CINLSFCLNA SGKHVADSIG FTVELQLDWQ KQKGGVRRAL FLASRQATLT QTLLIQNGAR EDCREMKIYL RNESEFRDKL SPIHIALNFS LDPQAPVDSH GLRPALHYQS KSRIEDKAQI LLDCGEDNIC VPDLQLEVFG EQNHVYLGDK NALNLTFHAQ NVGEGGAYEA ELRVTAPPEA EYSGLVRHPG NFSSLSCDYF AVNQSRLLVC DLGNPMKAGA SLWGGLRFTV PHLRDTKKTI QFDFQILSKN LNNSQSDVVS FRLSVEAQAQ VTLNGVSKPE AVLFPVSDWH PRDQPQKEED LGPAVHHVYE LINQGPSSIS QGVLELSCPQ ALEGQQLLYV TRVTGLNCTT NHPINPKGLE LDPEGSLHHQ QKREAPSRSS ASSGPQILKC PEAECFRLRC ELGPLHQQES QSLQLHFRVW AKTFLQREHQ PFSLQCEAVY KALKMPYRIL PRQLPQKERQ VATAVQWTKA EGSY. It is sometimes possible for the material contained within the vial of "Integrin alpha 5 (ITGA5/CD49e), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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