IF (Immunofluorescence)
(Immunofluorescence staining of THP-1 cell with AAA278955 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 490-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)
ITGAL recombinant antibody
ITGAL Recombinant Monoclonal Antibody
Gene Names
ITGAL; CD11A; LFA-1; LFA1A
Reactivity
reactivity
Applications
Immunofluorescence, Western Blot, ELISA
Purity
Affinity purified
Synonyms
ITGAL, Recombinant Antibody; ITGAL Recombinant Monoclonal Antibody; Integrin alpha-L (CD11 antigen-like family member A)(Leukocyte adhesion glycoprotein LFA-1 alpha chain)(LFA-1A)(Leukocyte function-associated molecule 1 alpha chain)(CD antigen CD11a); ITGAL; CD11A; ITGAL recombinant antibody
Host
HEK293F Cell
Reactivity
reactivity
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
2B2
Purity/Purification
Affinity purified
Form/Format
Liquid; PBS, 150mM NaCl, 0.02% sodium azide, 50% glycerol, pH 7.4.
Applicable Applications for ITGAL recombinant antibody
IF (Immunofluorescence), WB (Western Blot), ELISA
Immunogen Species
Human
Immunogen
A synthesized peptide derived from Human ITGAL
Conjugation
Non-conjugated
Research Area
Immunology; Signal transduction; Stem cells
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.
IF (Immunofluorescence)
(Immunofluorescence staining of THP-1 cell with AAA278955 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 490-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)
IF (Immunofluorescence)
(Immunofluorescence staining of THP-1 cell with AAA278955 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 490-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)
WB (Western Blot)
(Western BlotPositive WB detected in: THP-1 whole cell lysateAll lanes: ITGAL antibody at 1:2000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 129, 135, 120 kDaObserved band size: 130 kDa)
WB (Western Blot)
(Western BlotPositive WB detected in: THP-1 whole cell lysateAll lanes: ITGAL antibody at 1:2000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 129, 135, 120 kDaObserved band size: 130 kDa)
Related Product Information for ITGAL recombinant antibody
Integrin ITGAL/ITGB2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrin ITGAL/ITGB2 is a receptor for F11R (PubMed:11812992, PubMed:15528364). Integin ITGAL/ITGB2 is a receptor for the secreted form of ubiquitin-like protein ISG15; the interaction is mediated by ITGAL (PubMed:29100055). Involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes. Contributes to natural killer cell cytotoxicity (PubMed:15356110). Involved in leukocyte adhesion and transmigration of leukocytes including T-cells and neutrophils (PubMed:11812992). Required for generation of common lymphoid progenitor cells in bone marrow, indicating a role in lymphopoiesis (By similarity). Integrin ITGAL/ITGB2 in association with ICAM3, contributes to apoptotic neutrophil phagocytosis by macrophages (PubMed:23775590). {ECO:0000250|UniProtKB:P24063, ECO:0000269|PubMed:11812992, ECO:0000269|PubMed:15356110, ECO:0000269|PubMed:15528364, ECO:0000269|PubMed:23775590, ECO:0000269|PubMed:29100055}.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
Integrin alpha-L
NCBI Official Synonym Full Names
integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide)
NCBI Official Symbol
ITGAL
NCBI Official Synonym Symbols
CD11A; LFA-1; LFA1A
NCBI Protein Information
integrin alpha-L; LFA-1A; LFA-1 alpha; integrin gene promoter; CD11 antigen-like family member A; lymphocyte function-associated antigen 1; leukocyte adhesion glycoprotein LFA-1 alpha chain; leukocyte function-associated molecule 1 alpha chain; antigen CD
UniProt Protein Name
Integrin alpha-L
UniProt Gene Name
ITGAL
UniProt Synonym Gene Names
CD11A; LFA-1A
UniProt Entry Name
ITAL_HUMAN
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Product Notes
The ITGAL itgal (Catalog #AAA278955) is a Recombinant Antibody produced from HEK293F Cell and is intended for research purposes only. The product is available for immediate purchase. The ITGAL Recombinant Monoclonal Antibody reacts with reactivity and may cross-react with other species as described in the data sheet. AAA Biotech's ITGAL can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the ITGAL itgal for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ITGAL, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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