Mouse KCNQ4 Monoclonal Antibody | anti-KCNQ4 antibody

KCNQ4 Antibody

Cat. Num. AAA103472

• Gene Names: KCNQ4; DFNA2; KV7.4; DFNA2A
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Immunoprecipitation, Western Blot
• Purity: Protein G Purified
• Synonyms: KCNQ4, Antibody; KCNQ4 Antibody; DFNA 2 antibody, DFNA2 antibody, KCNQ 4 antibody, Kcnq4 antibody, KCNQ4_HUMAN antibody, KQT like 4 antibody, KQT-like 4 antibody, KV7.4 antibody, Potassium channel KQT like 4 antibody, Potassium channel subunit alpha KvLQT4 antibody, Potassium voltage gated channel KQT like protein 4 antibody, Potassium voltage gated channel KQT like subfamily member 4 antibody, Potassium voltage gated channel subfamily KQT member 4 antibody, Potassium voltage-gated channel subfamily KQT member 4 antibody, Voltage gated potassium channel subunit Kv7.4 antibody, Voltage-gated potassium channel subunit Kv7.4 antibody ; anti-KCNQ4 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: S43-6
• Specificity: Detects ~77kDa
• Purity/Purification: Protein G Purified
• Form/Format: PBS pH7.4, 50% glycerol, 0.09% sodium azide
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 695

• Applicable Applications for anti-KCNQ4 antibody: AM (Antibody Microarray), IHC (Immunohistochemistry), IF (Immunofluorescence), ICC (Immunocytochemistry), IP (Immunoprecipitation), WB (Western Blot)
• Immunogen: Fusion protein amin o acids 2-77 of human KCNQ4 Cellular Localization Plasma Membrane | Basal Cell Membrane
• Research Area: Cancer, Ion Channels, Neuroscience, Potassium Channels, Voltage-Gated Potassium Channels
• Certificate of Analysis: 1 ug/mL was sufficient for detection of KCNQ4 in 10ug of COS-1 cell lysate transiently expressing KCNQ4 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Preparation and Storage: Store at -20°C. Avoid freeze/ thaw cycles

IHC (Immunohistochemisry)

(Immunohistochemistry analysis using Mouse Anti-KCNQ4 Monoclonal Antibody, Clone S43-6. Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-KCNQ4 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.)

WB (Western Blot)

(Western Blot analysis of Rat tissue lysate showing detection of KCNQ4 protein using Mouse Anti-KCNQ4 Monoclonal Antibody, Clone S43-6. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-KCNQ4 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)

IHC (Immunohistochemistry)

(Immunohistochemistry analysis using Mouse Anti-KCNQ4 Monoclonal Antibody, Clone S43-6. Tissue: frozen brain section. Species: mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-KCNQ4 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 ul for 5 minutes at RT.)

Related Product Information for anti-KCNQ4 antibody

Scientific Background: Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient (1). They are present in the membranes that surround all biological cells because their main function is to regulate the flow of ions across this membrane. Whereas some ion channels permit the passage of ions based on charge, others conduct based on a ionic species, such as sodium or potassium. Furthermore, in some ion channels, the passage is governed by a gate which is controlled by chemical or electrical signals, temperature, or mechanical forces. There are a few main classifications of gated ion channels. There are voltage- gated ion channels, ligand- gated, other gating systems and finally those that are classified differently, having more exotic characteristics. The first are voltage- gated ion channels which open and close in response to membrane potential. These are then separated into sodium, calcium, potassium, proton, transient receptor, and cyclic nucleotide-gated channels; each of which is responsible for a unique role. Ligand-gated ion channels are also known as ionotropic receptors, and they open in response to specific ligand molecules binding to the extracellular domain of the receptor protein. The other gated classifications include activation and inactivation by second messengers, inward-rectifier potassium channels, calcium-activated potassium channels, two-pore-domain potassium channels, light-gated channels, mechano-sensitive ion channels and cyclic nucleotide-gated channels. Finally, the other classifications are based on less normal characteristics such as two-pore channels, and transient receptor potential channels (2). Specifically, the protein encoded by this gene forms a potassium channel that is thought to play a critical role in the regulation of neuronal excitability (3), particularly in sensory cells of the cochlea (4). The current generated by this channel is inhibited by M1 muscarinic acetylcholine receptors and activated by retigabine, a novel anti-convulsant drug (5).

Product Categories/Family for anti-KCNQ4 antibody

Ion Channels; Neuroscience

References

1. Hille B. (2001) Ion Channels of Excitable Membranes, 3rd Ed., Sinauer Associated Inc.: Sunderland, MA USA. 2. www.iochannels.org 3. Hernandez C.C., Zaiko O., Tolstykh G.P., Shapiro M.S. (2008) J Physiol. 586(7): 1811-1821. 4. Kharkovets T., et al. (2006) EMBO J. 25(3): 642-652. 5. Tatulian L., Delmas P., Abogadie F.C., Brown D.A. (2001) J Neuroscience. 21(15): 5535-5545.

NCBI and Uniprot Product Information

• NCBI GI #: 26638653
• NCBI GeneID: 9132
• NCBI Accession #: NP_004691.2
• NCBI GenBank Nucleotide #: NM_004700.3
• UniProt Accession #: P56696; O96025
• Molecular Weight: 71,196 Da
• NCBI Official Full Name: potassium voltage-gated channel subfamily KQT member 4 isoform a
• NCBI Official Synonym Full Names: potassium voltage-gated channel, KQT-like subfamily, member 4
• NCBI Official Symbol: KCNQ4
• NCBI Official Synonym Symbols: DFNA2; KV7.4; DFNA2A
• NCBI Protein Information: potassium voltage-gated channel subfamily KQT member 4; potassium channel KQT-like 4; potassium channel subunit alpha KvLQT4
• UniProt Protein Name: Potassium voltage-gated channel subfamily KQT member 4
• UniProt Gene Name: KCNQ4
• UniProt Entry Name: KCNQ4_HUMAN