IF (Immunofluorescence)
(ICC/IF analysis of KRT20 in A549 cells, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human KRT20 antibody (1:200) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Mouse anti-Human KRT20 Monoclonal Antibody | anti-KRT20 antibody
KRT20 antibody
Gene Names
KRT20; K20; CD20; CK20; CK-20; KRT21
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunocytochemistry, Western Blot, ELISA
Purity
By protein-A affinity chromatography
Synonyms
KRT20, Antibody; KRT20 antibody; Keratin type I cytoskeletal 20; Keratin; type I cytoskeletal 20; CD20; CK-20; CK20; K20; KRT21; anti-KRT20 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a, k
Clone Number
AT10D8
Purity/Purification
By protein-A affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
424
Applicable Applications for anti-KRT20 antibody
FCM/FACS (Flow Cytometry), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot), ELISA
Antigen Species
Human
Immunogen
Recombinant human KRT20 (1-424aa) purified from E Coli.
Clone Note
Anti-human KRT20 mAb, clone AT10D8, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human KRT20 protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
IF (Immunofluorescence)
(ICC/IF analysis of KRT20 in A549 cells, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human KRT20 antibody (1:200) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
IF (Immunofluorescence)
(ICC/IF analysis of KRT20 in A549 cells, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human KRT20 antibody (1:200) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
WB (Western Blot)
(The Recombinant Human KRT20 (25ng) and Cell lysates of HeLa, A431 (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRT20 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant Human KRT20 Lane 2.: HeLa cell lysate Lane 3.: A431 cell lysate)
WB (Western Blot)
(The Recombinant Human KRT20 (25ng) and Cell lysates of HeLa, A431 (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRT20 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant Human KRT20 Lane 2.: HeLa cell lysate Lane 3.: A431 cell lysate)
Related Product Information for anti-KRT20 antibody
Keratin 20 is a type I cytokeratin. It is a major cellular protein of mature enterocytes and goblet cells and is specifically found in the gastric and intestinal mucosa. In immunohistochemistry, antibodies to KRT20 can be used to identify a range of adenocarcinoma arising from epithelia that normally contain the KRT20 protein. For example, the protein is commonly found in colorectal cancer, transitional cell carcinomas and in Merkel cell carcinoma, but is absent in lung cancer, prostate cancer, and non-mucinous ovarian cancer. It is often used in combination with antibodies to CK7 to distinguish different types of glandular tumor.
Product Categories/Family for anti-KRT20 antibody
References
Moll R., et al. (1990) J Cell Biol. 111 (2): 567-580; Piero D., et al. (2011) Nat Biotechnol. 29 (12): 1120-1127
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
48,487 Da
NCBI Official Full Name
keratin, type I cytoskeletal 20
NCBI Official Synonym Full Names
keratin 20
NCBI Official Symbol
KRT20
NCBI Official Synonym Symbols
K20; CD20; CK20; CK-20; KRT21
NCBI Protein Information
keratin, type I cytoskeletal 20
UniProt Protein Name
Keratin, type I cytoskeletal 20
UniProt Gene Name
KRT20
UniProt Synonym Gene Names
CK-20; K20
Similar Products
Product Notes
The KRT20 krt20 (Catalog #AAA48199) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The KRT20 antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's KRT20 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the KRT20 krt20 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KRT20, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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