IF (Immunofluorescence)
(Immunofluorescent analysis of LIN28A staining in human testis cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
Mouse anti-Human LIN28A Monoclonal Antibody | anti-LIN28A antibody
Anti-LIN28A Antibody
Gene Names
LIN28A; CSDD1; LIN28; LIN-28; ZCCHC1; lin-28A
Reactivity
Human
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
This antibody is purified through a protein G column.
Synonyms
LIN28A, Antibody; Anti-LIN28A Antibody; anti-LIN28A antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG1 kappa
Specificity
Recognizes endogenous levels of LIN28A protein.
Purity/Purification
This antibody is purified through a protein G column.
Form/Format
Mouse IgG1 kappa. Liquid in PBS, pH7.3, 30% glycerol, and 0.01% sodium azide.
Applicable Applications for anti-LIN28A antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
Recombinant fusion protein of human LIN28A. The exact sequence is proprietary.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
IF (Immunofluorescence)
(Immunofluorescent analysis of LIN28A staining in human testis cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
IF (Immunofluorescence)
(Immunofluorescent analysis of LIN28A staining in human testis cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of LIN28A staining in human testis formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of LIN28A staining in human testis formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
WB (Western Blot)
(Western blot analysis of LIN28A expression in LIN28 recombinant protein (A) whole cell lysates. (Predicted band size: 22 kD; Observed band size: 34 kD))
WB (Western Blot)
(Western blot analysis of LIN28A expression in LIN28 recombinant protein (A) whole cell lysates. (Predicted band size: 22 kD; Observed band size: 34 kD))
Related Product Information for anti-LIN28A antibody
Mouse monoclonal antibody to LIN28A
Product Categories/Family for anti-LIN28A antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
22,743 Da
NCBI Official Full Name
protein lin-28 homolog A
NCBI Official Synonym Full Names
lin-28 homolog A (C. elegans)
NCBI Official Symbol
LIN28A
NCBI Official Synonym Symbols
CSDD1; LIN28; LIN-28; ZCCHC1; lin-28A
NCBI Protein Information
protein lin-28 homolog A; RNA-binding protein LIN-28; zinc finger CCHC domain-containing protein 1; zinc finger, CCHC domain containing 1
UniProt Protein Name
Protein lin-28 homolog A
UniProt Gene Name
LIN28A
UniProt Synonym Gene Names
CSDD1; LIN28; ZCCHC1; Lin-28A
UniProt Entry Name
LN28A_HUMAN
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Product Notes
The LIN28A lin28a (Catalog #AAA266110) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-LIN28A Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's LIN28A can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the LIN28A lin28a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LIN28A, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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