IF (Immunofluorescence)
(Immunofluorescence analysis of LIN28A Monoclonal Antibody with paraffin-embedded human testis tissue. 0.025 mg/ml primary antibody was followed by PE-conjugated goat anti-mouse lgG (whole molecule). PE emits orange fluorescence.)
Mouse anti-Human LIN28A Monoclonal Antibody | anti-LIN28A antibody
LIN28A Antibody
Gene Names
LIN28A; CSDD1; LIN28; LIN-28; ZCCHC1; lin-28A
Reactivity
Human
Applications
Immunofluorescence, Immunohistochemistry, ELISA, Western Blot
Purity
Purified Mouse Monoclonal Antibody (Mab)
Synonyms
LIN28A, Antibody; LIN28A Antibody; Protein lin-28 homolog A; Lin-28A; Zinc finger CCHC domain-containing protein 1; LIN28A; CSDD1; LIN28; ZCCHC1; anti-LIN28A antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1 Kappa
Clone Number
55CT58.12.1
Specificity
LIN28A recombinant protein is used to produce this monoclonal antibody.
Purity/Purification
Purified Mouse Monoclonal Antibody (Mab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Length
209
Applicable Applications for anti-LIN28A antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Recombinant Protein
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IF (Immunofluorescence)
(Immunofluorescence analysis of LIN28A Monoclonal Antibody with paraffin-embedded human testis tissue. 0.025 mg/ml primary antibody was followed by PE-conjugated goat anti-mouse lgG (whole molecule). PE emits orange fluorescence.)
IF (Immunofluorescence)
(Immunofluorescence analysis of LIN28A Monoclonal Antibody with paraffin-embedded human testis tissue. 0.025 mg/ml primary antibody was followed by PE-conjugated goat anti-mouse lgG (whole molecule). PE emits orange fluorescence.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human testis tissue reacted with LIN28A Monoclonal Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human testis tissue reacted with LIN28A Monoclonal Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of LIN28 recombinant protein by anti-LIN28A Monoclonal Antibody. LIN28 (arrow) was detected using the purified Mab.)
WB (Western Blot)
(Western blot analysis of LIN28 recombinant protein by anti-LIN28A Monoclonal Antibody. LIN28 (arrow) was detected using the purified Mab.)
Product Categories/Family for anti-LIN28A antibody
References
Pluripotency factors Lin28 and Oct4 identify a sub-population of stem cell-like cells in ovarian cancer. Peng S, et al. Oncogene, 2010 Apr 8. PMID 20101213.
Lin28-mediated post-transcriptional regulation of Oct4 expression in human embryonic stem cells. Qiu C, et al. Nucleic Acids Res, 2010 Mar. PMID 19966271.
An epigenetic switch involving NF-kappaB, Lin28, Let-7 MicroRNA, and IL6 links inflammation to cell transformation. Iliopoulos D, et al. Cell, 2009 Nov 13. PMID 19878981.
TUT4 in concert with Lin28 suppresses microRNA biogenesis through pre-microRNA uridylation. Heo I, et al. Cell, 2009 Aug 21. PMID 19703396.
A role for Lin28 in primordial germ-cell development and germ-cell malignancy. West JA, et al. Nature, 2009 Aug 13. PMID 19578360.
Lin28-mediated post-transcriptional regulation of Oct4 expression in human embryonic stem cells. Qiu C, et al. Nucleic Acids Res, 2010 Mar. PMID 19966271.
An epigenetic switch involving NF-kappaB, Lin28, Let-7 MicroRNA, and IL6 links inflammation to cell transformation. Iliopoulos D, et al. Cell, 2009 Nov 13. PMID 19878981.
TUT4 in concert with Lin28 suppresses microRNA biogenesis through pre-microRNA uridylation. Heo I, et al. Cell, 2009 Aug 21. PMID 19703396.
A role for Lin28 in primordial germ-cell development and germ-cell malignancy. West JA, et al. Nature, 2009 Aug 13. PMID 19578360.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
22743
NCBI Official Full Name
protein lin-28 homolog A
NCBI Official Synonym Full Names
lin-28 homolog A (C. elegans)
NCBI Official Symbol
LIN28A
NCBI Official Synonym Symbols
CSDD1; LIN28; LIN-28; ZCCHC1; lin-28A
NCBI Protein Information
protein lin-28 homolog A
UniProt Protein Name
Protein lin-28 homolog A
UniProt Gene Name
LIN28A
UniProt Synonym Gene Names
CSDD1; LIN28; ZCCHC1; Lin-28A
UniProt Entry Name
LN28A_HUMAN
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Product Notes
The LIN28A lin28a (Catalog #AAA283419) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The LIN28A Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's LIN28A can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the LIN28A lin28a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LIN28A, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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