Mouse MAP2 (Microtubule Associated Protein 2) Monoclonal Antibody | anti-MAP2 antibody

MAP2 (Microtubule Associated Protein 2)

Cat. Num. AAA76730

• Gene Names: MAP2; MAP2A; MAP2B; MAP2C
• Reactivity: Human, Mouse, Rat, Bovine
• Applications: Immunofluorescence, Immunohistochemistry, Western Blot, Immunocytochemistry
• Synonyms: MAP2 (Microtubule Associated Protein 2), Antibody; MAP2 (Microtubule Associated Protein 2); anti-MAP2 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat, Bovine
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Form/Format: Liquid. Purified antibody at 1mg/mL in 50% PBS, 50% glycerol plus 5mM NaN3

• Applicable Applications for anti-MAP2 antibody: IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ICC (Immunocytochemistry)
• Immunogen: Full length MAP2A/B purified from bovine spinal cord
• Preparation and Storage: Antibody can also be aliquotted and stored frozen at -20 degree C in a manual defrost freezer for sixmonths without detectable loss of activity. The antibody can be stored at 2 degree - 8 degree C for 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles.

WB (Western Blot)

(Western blot analysis of tissue and cell lysates using mouse mAb to MAP2, AAA76730, dilution 1:10,000 in green: [1] protein standard (red), [2] rat brain, [3] mouse brain, and [4] embryonic rat cortical neuron-glial cell lysate. A band at about 280 kDa corresponds to the MAP2A and MAP2B proteins.)

IF (Immunofluorescence)

(Immunofluorescent analysis of a rat hippocampus section stained with mouse mAb to MAP2, AAA76730, dilution 1:2,000 in green, and costained with rabbit pAb to FOX3/NeuN in red. AAA76730 labels MAP2 protein in the perikarya and dendrites of most neurons while the FOX3/NeuN antibody selectively stains nuclei and proximal soma of neuronal cells.)

Related Product Information for anti-MAP2 antibody

Microtubules are 25nm diameter protein rods found in most kinds of eukaryotic cells and are associated with a family of proteins called microtubule associated proteins (MAPs). MAPs play a crucial role in the regulation of microtubule dynamics and interactions in vivo. MAP2 was originally named as one of the higher molecular weight MAPs with an SDS-PAGE molecular weight of about 280kDa. MAP2 isoforms are expressed only in neurons, specifically in the perikarya and dendrites of these cells. Antibodies to MAP2 isotypes are therefore excellent markers of neuronal dendrites and are useful for identifying neurons in cell culture and sections.

This antibody was raised against purified full length bovine brain MAP2 and the epitope was mapped to amino acids 631-1056 of the human sequence.

NCBI and Uniprot Product Information

• NCBI GI #: 87578396
• NCBI GeneID: 4133
• NCBI Accession #: NP_002365.3
• NCBI GenBank Nucleotide #: NM_002374.3
• UniProt Accession #: P11137; Q17S04; Q8IUX2; Q99975; Q99976
• Molecular Weight: 199,526 Da
• NCBI Official Full Name: microtubule-associated protein 2 isoform 1
• NCBI Official Synonym Full Names: microtubule-associated protein 2
• NCBI Official Symbol: MAP2
• NCBI Official Synonym Symbols: MAP2A; MAP2B; MAP2C
• NCBI Protein Information: microtubule-associated protein 2; MAP-2
• UniProt Protein Name: Microtubule-associated protein 2
• UniProt Gene Name: MAP2
• UniProt Synonym Gene Names: MAP-2
• UniProt Entry Name: MTAP2_HUMAN