Mouse anti-Human MHC Class II Monoclonal Antibody | anti-HLA-DQB1 antibody

Mouse Monoclonal [clone TDR31.1] (IgG1) to Human MHC Class II

Cat. Num. AAA51942

• Gene Names: HLA-DQB1; IDDM1; CELIAC1; HLA-DQB
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Western Blot, Immunohistochemistry, Immunohistochemistry
• Purity: Protein A Purified
• Synonyms: MHC Class II, Antibody; Mouse Monoclonal [clone TDR31.1] (IgG1) to Human MHC Class II; Anti-MHC Class II Antibody (clone TDR31.1) IHC-plus; MHC Class II; Human MHC Class II; anti-HLA-DQB1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: TDR31.1
• Specificity: Monomorphic class II beta chain epitope.
• Purity/Purification: Protein A Purified
• Form/Format: 50 mM sodium phosphate, pH 7.5, 100 mM potassium chloride, 150 mM sodium chloride, 0.5 mg/ml Gentamicin sulfate. Sourced in TCS.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 229

• Applicable Applications for anti-HLA-DQB1 antibody: FCM/FACS (Flow Cytometry), WB (Western Blot), IHC (Immunohistochemistry), IHC (Immunohistochemistry)
• Immunogen Description: Purified (With Antibiotics) class II from JY cell line.
• Immunogen Species: MHC class ll antibody was raised against Human
• Immunogen Type: Cells
• Immunogen: MHC Class II antibody was raised against purified (With Antibiotics) class II from JY cell line.
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and AAA Biotech makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Store at +4 degree C. DO NOT FREEZE.

FCM/FACS (Flow Cytometry)

(Flow cytometry of MHC Class II antibody)

IHC (Immunohistochemistry)

(Anti-MHC Class II antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

NCBI and Uniprot Product Information

• NCBI GI #: 1160938
• NCBI GeneID: 3119
• NCBI Official Full Name: MHC class II, partial
• NCBI Official Synonym Full Names: major histocompatibility complex, class II, DQ beta 1
• NCBI Official Symbol: HLA-DQB1
• NCBI Official Synonym Symbols: IDDM1; CELIAC1; HLA-DQB
• NCBI Protein Information: HLA class II histocompatibility antigen, DQ beta 1 chain; MHC DQ beta; MHC class2 antigen; lymphocyte antigen; MHC class II antigen DQB1; MHC class II DQ beta chain; MHC class II antigen HLA-DQ-beta-1; MHC class II HLA-DQ beta glycoprotein
• UniProt Protein Name: HLA class II histocompatibility antigen, DQ beta 1 chain
• UniProt Gene Name: HLA-DQB1
• UniProt Synonym Gene Names: HLA-DQB
• UniProt Entry Name: DQB1_HUMAN