Application Data
(Staining of rat splenocytes with Mouse anti Rat MHC class II RT1B)
Mouse MHC CLASS II RT1B Monoclonal Antibody
MOUSE ANTI RAT MHC CLASS II RT1B:FITC
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Flow Cytometry: Minimum Dilution: Neat; Maximum Dilution: 1/10
1% Bovine Serum Albumin
Preparation
Fusion Partners
Shelf Life: 18 months from date of despatch.
Application Data
(Staining of rat splenocytes with Mouse anti Rat MHC class II RT1B)
Application Data
(Staining of rat splenocytes with Mouse anti Rat MHC class II RT1B)
Application Data
(Staining of rat spleen cells with Mouse anti Rat RT1B)
Application Data
(Staining of rat spleen cells with Mouse anti Rat RT1B)
Application Data
(Staining of rat spleen cells with Mouse anti Rat RT1B: FITC)
Application Data
(Staining of rat spleen cells with Mouse anti Rat RT1B: FITC)
Application Data
(Staining of rat spleen cells with Mouse anti Rat RT1B: RPE)
Application Data
(Staining of rat spleen cells with Mouse anti Rat RT1B: RPE)
Application Data
(Staining of rat spleen cells with Mouse anti Rat RT1B: FITC)
Application Data
(Staining of rat spleen cells with Mouse anti Rat RT1B: FITC)
Application Data
(Rat Spleen Tissue lysate probed with Mouse anti Rat MHC Class II RT1B)
Application Data
(Rat Spleen Tissue lysate probed with Mouse anti Rat MHC Class II RT1B)
Application Data
(Staining of rat spleen cells with Mouse anti Rat RTIB: Alexa Fluor 647)
Application Data
(Staining of rat spleen cells with Mouse anti Rat RTIB: Alexa Fluor 647)
Application Data
(Staining of rat spleen cells with Mouse anti Rat RT1B: Alexa Fluor 488)
Application Data
(Staining of rat spleen cells with Mouse anti Rat RT1B: Alexa Fluor 488)
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Product Notes
The MHC CLASS II RT1B (Catalog #AAA11878) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's MHC CLASS II RT1B can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry). Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. Flow Cytometry: Minimum Dilution: Neat; Maximum Dilution: 1/10. Researchers should empirically determine the suitability of the MHC CLASS II RT1B for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MHC CLASS II RT1B, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Frequently Asked Questions
Is this RT1B monoclonal antibody suitable for flow cytometry or immunostaining?
Yes, the MHC Class II RT1B antibody (Clone OX-6) is specifically validated for flow cytometry applications. It comes in FITC-conjugated format and is routinely tested on rat splenocytes. The antibody can also be used in functional assays, making it versatile for multiple immunological techniques.
Which mouse strains or species does this antibody detect?
This antibody recognizes rat MHC Class II RT1B antigen present on B lymphocytes, dendritic cells, some macrophages, and epithelial cells. However, it does not react with the rat BDIX strain due to a defect in RT1B expression. The antibody also cross-reacts with mouse strains carrying H-2 haplotypes k and s.
What is the clone number and isotype of the RT1B antibody?
The clone number is OX-6 and the isotype is IgG1. The antibody is monoclonal and derived from mouse hybridomas, providing high specificity for MHC Class II RT1B. The FITC-conjugated format has an IgG concentration of 0.1 mg/mL (varies by lot).
What dilution is recommended for cell staining or tissue applications?
For flow cytometry, use 10 µL of the working dilution to label 10⁶ cells in 100 µL buffer. The minimum dilution is neat (undiluted), with a maximum recommended dilution of 1/10. Exact dilutions should be optimized empirically for specific applications.
Can this antibody be used for MHC Class II expression analysis on immune cells?
Yes, this antibody is ideal for analyzing MHC Class II expression on immune cells. It specifically detects RT1B on B lymphocytes, dendritic cells, and macrophages, making it excellent for characterizing immune cell populations and analyzing MHC Class II antigen expression patterns.
What storage conditions are required to maintain antibody stability?
Store the antibody at 4°C or -20°C undiluted, with a shelf life of 18 months from dispatch. Avoid frost-free freezers and repeated freeze-thaw cycles, as these can denature the antibody. The product is photosensitive and should be protected from light. Use a microcentrifuge if a precipitate appears before use.
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