ICC (Immunocytochemistry)
(Immunocytochemistry of MLH1 (green) in hela using MLH1 Rabbit mAb at dilution 1/50, and DAPI(blue))
anti-Human MLH1 Monoclonal Antibody | anti-MLH1 antibody
MLH1 Rabbit mAb
Gene Names
MLH1; FCC2; COCA2; HNPCC; hMLH1; HNPCC2
Reactivity
Human
Applications
Immunofluorescence, Western Blot
Purity
Affinity Purified
Synonyms
MLH1, Antibody; MLH1 Rabbit mAb; FCC2; COCA2; HNPCC; hMLH1; HNPCC2; anti-MLH1 antibody
Host
Recombinant Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG
Clone Number
S07-5D3
Purity/Purification
Affinity Purified
Form/Format
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Concentration
0.3mg/ml (varies by lot)
Applicable Applications for anti-MLH1 antibody
IF (Immunofluorescence), WB (Western Blot)
Immunogen
Recombinant protein of human MLH1
Conjugation
Unconjugated
Modification
Unmodification
Preparation and Storage
Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze/thaw cycles.
ICC (Immunocytochemistry)
(Immunocytochemistry of MLH1 (green) in hela using MLH1 Rabbit mAb at dilution 1/50, and DAPI(blue))
ICC (Immunocytochemistry)
(Immunocytochemistry of MLH1 (green) in hela using MLH1 Rabbit mAb at dilution 1/50, and DAPI(blue))
WB (Western Blot)
(Western blot detection of MLH1 in Hela,A549,HL-60,U2OS cell lysates using MLH1 Rabbit mAb(1:1000 diluted).Predicted band size:85kDa.Observed band size:85kDa.)
WB (Western Blot)
(Western blot detection of MLH1 in Hela,A549,HL-60,U2OS cell lysates using MLH1 Rabbit mAb(1:1000 diluted).Predicted band size:85kDa.Observed band size:85kDa.)
Related Product Information for anti-MLH1 antibody
The protein encoded by this gene can heterodimerize with mismatch repair endonuclease PMS2 to form MutL alpha, part of the DNA mismatch repair system. When MutL alpha is bound by MutS beta and some accessory proteins, the PMS2 subunit of MutL alpha introduces a single-strand break near DNA mismatches, providing an entry point for exonuclease degradation. The encoded protein is also involved in DNA damage signaling and can heterodimerize with DNA mismatch repair protein MLH3 to form MutL gamma, which is involved in meiosis. This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). [provided by RefSeq, Aug 2017]
Product Categories/Family for anti-MLH1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
84,601 Da
NCBI Official Full Name
DNA mismatch repair protein Mlh1 isoform 1
NCBI Official Synonym Full Names
mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli)
NCBI Official Symbol
MLH1
NCBI Official Synonym Symbols
FCC2; COCA2; HNPCC; hMLH1; HNPCC2
NCBI Protein Information
DNA mismatch repair protein Mlh1
UniProt Protein Name
DNA mismatch repair protein Mlh1
UniProt Gene Name
MLH1
UniProt Synonym Gene Names
COCA2
UniProt Entry Name
MLH1_HUMAN
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Product Notes
The MLH1 mlh1 (Catalog #AAA314550) is an Antibody produced from Recombinant Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MLH1 Rabbit mAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MLH1 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the MLH1 mlh1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MLH1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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