IF (Immunofluorescence)
(Figure 3. Confocal immunofluorescence analysis of Hela cells using MLH1 mouse mAb (green), showing nuclear localization. Red. Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)
Mouse MLH1 Antibody | anti-MLH1 antibody
MLH1 Antibody
Gene Names
MAP3K11; MLK3; PTK1; SPRK; MLK-3; MEKK11
Reactivity
Human, Monkey
Applications
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Synonyms
MLH1; N/A; MLH1 Antibody; FCC2; COCA2; HNPCC; anti-MLH1 antibody
Host
Mouse
Reactivity
Human, Monkey
Isotype
IgG1
Clone Number
4C9C7
Form/Format
Ascitic fluid containing 0.03% sodium azide.
Sequence Length
847
Applicable Applications for anti-MLH1 antibody
ELISA, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
Purified recombinant fragment of ABL1 (aa577-650) expressed in E. Coli.
Preparation and Storage
Store at 4 degree C, for long term storage, store at-20 degree C
IF (Immunofluorescence)
(Figure 3. Confocal immunofluorescence analysis of Hela cells using MLH1 mouse mAb (green), showing nuclear localization. Red. Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)
IF (Immunofluorescence)
(Figure 3. Confocal immunofluorescence analysis of Hela cells using MLH1 mouse mAb (green), showing nuclear localization. Red. Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)
IHC (Immunohiostchemistry)
(Figure 2. Immunohistochemical analysis of paraffin-embedded human rectum cancer (left) and ovarian cancer (right) tissues, showing nuclear localization with DAB staining using MLH1 mouse mAb.)
IHC (Immunohiostchemistry)
(Figure 2. Immunohistochemical analysis of paraffin-embedded human rectum cancer (left) and ovarian cancer (right) tissues, showing nuclear localization with DAB staining using MLH1 mouse mAb.)
WB (Western Blot)
(Figure 1. Western blot analysis using MLH1 mouse mAb against Hela (1), MCF-7 (2) and A549 (3), Jurkat (4), 2R75 (5) and COS (6) cell lysate.)
WB (Western Blot)
(Figure 1. Western blot analysis using MLH1 mouse mAb against Hela (1), MCF-7 (2) and A549 (3), Jurkat (4), 2R75 (5) and COS (6) cell lysate.)
Related Product Information for anti-MLH1 antibody
DNA-mismatch repair (MMR), a conserved process that involves correcting errors made during DNA synthesis, is crucial to the maintenance of genomic integrity. Lack of a functional DNA-mismatch repair pathway is a common characteristic of several different types of human cancers, either due to an MMR gene mutation or promoter-methylation gene silencing. MLH1 is a human homolog of the E. coli DNA mismatch repair gene mutL, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in hereditary nonpolyposis colon cancer (HNPCC). MLH1 is an integral part of the protein complex responsible for mismatch repair expressed in lymphocytes, heart, colon, breast, lung, spleen, testis, prostate, thyroid and gall bladder, and is methylated in several ovarian tumors. Loss of MLH1 protein expression is associated with a mutated phenotype, microsatellite instability and a predisposition to cancer.
Product Categories/Family for anti-MLH1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
85kDa
NCBI Official Full Name
mitogen-activated protein kinase kinase kinase 11
NCBI Official Synonym Full Names
mitogen-activated protein kinase kinase kinase 11
NCBI Official Symbol
MAP3K11
NCBI Official Synonym Symbols
MLK3; PTK1; SPRK; MLK-3; MEKK11
NCBI Protein Information
mitogen-activated protein kinase kinase kinase 11
UniProt Protein Name
Mitogen-activated protein kinase kinase kinase 11
UniProt Gene Name
MAP3K11
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Product Notes
The MLH1 map3k11 (Catalog #AAA108705) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MLH1 Antibody reacts with Human, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's MLH1 can be used in a range of immunoassay formats including, but not limited to, ELISA, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the MLH1 map3k11 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MLH1, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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