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Mouse anti-Human MUTYH Monoclonal Antibody | anti-MUTYH antibody
MUTYH (A/G-specific Adenine DNA Glycosylase, MutY Homolog, hMYH, MYH) (HRP)
Gene Names
MUTYH; MYH
Reactivity
Human
Applications
ELISA, Immunoprecipitation, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
MUTYH, Antibody; MUTYH (A/G-specific Adenine DNA Glycosylase, MutY Homolog, hMYH, MYH) (HRP); anti-MUTYH antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
4D10
Specificity
Recognizes human MUTYH.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Applicable Applications for anti-MUTYH antibody
ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
Partial recombinant corresponding to aa436-535 from human MUTYH (AAH03178) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
KLTYQVYGLALEGQTPVTTVPPGARWLTQEEFHTAAVSTAMKKVFRVYQGQQPGTCMGSKRSQVSSPCSRKKPRMGQQVLDNFFRSHISTDAHSLNSAAQ
Conjugate
HRP
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
WB (Western Blot)
(Western blot analysis of MUTYH over-expressed 293 cell line, cotransfected with MUTYH Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with MUTYH monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)
Application Data
(Detection limit for recombinant GST tagged MUTYH is ~1ng/ml as a capture antibody.)
IP (Immunoprecipitation)
(Immunoprecipitation of MUTYH transfected lysate using anti-MUTYH monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with MUTYH rabbit polyclonal antibody.)
WB (Western Blot)
(Western Blot analysis of MUTYH expression in transfected 293T cell line by MUTYH monoclonal antibody. Lane 1: MUTYH transfected lysate (59.1kD). Lane 2: Non-transfected lysate.)
WB (Western Blot)
(MUTYH monoclonal antibody Western Blot analysis of MUTYH expression in HeLa NE.)
WB (Western Blot)
(Western Blot detection against Immunogen (36.74kD).)
Related Product Information for anti-MUTYH antibody
MYH is the eukaryotic homolog to the E. coli protein MutY. It was first identified in HeLa cells and nicks DNA replication errors, specifically A/G mismatches.
Product Categories/Family for anti-MUTYH antibody
References
1. Cancer-associated variants and a common polymorphism of MUTYH exhibit reduced repair of oxidative DNA damage using a GFP-based assay in mammalian cells. Raetz AG, Xie Y, Kundu S, Brinkmeyer MK, Chang C, David SS.Carcinogenesis. 2012 Sep 20. 2. Reduced expression of MUTYH with suppressive activity against mutations caused by 8-hydroxyguanine is a novel predictor of a poor prognosis in human gastric cancer. Shinmura K, Goto M, Suzuki M, Tao H, Yamada H, Igarashi H, Matsuura S, Maeda M, Konno H, Matsuda T, Sugimura H.The Journal of Pathology DOI: 10.1002 /path.2953. 3. Effects of base excision repair proteins on mutagenesis by 8-oxo-7,8-dihydroguanine (8-hydroxyguanine) paired with cytosine and adenine. Suzuki T, Harashima H, Kamiya H.DNA Repair (Amst). 2010 Feb 28. 4. Immunohistochemistry is not an accurate first step towards the molecular diagnosis of MUTYH-associated polyposis. van der Post RS, Kets CM, Ligtenberg MJ, van Krieken JH, Hoogerbrugge N.Virchows Arch. 2009 Jan;454(1):25-9. Epub 2008 Nov 25.
