Mouse anti-Human MXI1 Monoclonal Antibody | anti-MXI1 antibody

MXI1 Mouse Monoclonal Antibody [Clone PCRP-MXI1-1A3]

Cat. Num. AAA215821

• Gene Names: MXI1; MXI; MAD2; MXD2; bHLHc11
• Reactivity: Human
• Applications: Western Blot, Immunofluorescence, Flow Cytometry, Functional Assay
• Synonyms: MXI1, Antibody; MXI1 Mouse Monoclonal Antibody [Clone PCRP-MXI1-1A3]; bHLHc11; Class C basic helix-loop-helix protein 11; MAX dimerization protein 2 (MAD2); MAX interacting protein 1; Max interactor 1; Max related transcription factor; MXD2; MXI; MXI1 protein; anti-MXI1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse/IgG2b
• Clone Number: PCRP-MXI1-1A3
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0. 05% BSA & 0. 05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Concentration: Purified Ab with BSA and Azide at 200ug/ml; OR; Purified Ab WITHOUT BSA and Azide at 1.0mg/ml (varies by lot)

• Applicable Applications for anti-MXI1 antibody: WB (Western Blot), IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
• Immunogen: Recombinant full-length human MXI1 protein
• Cellular Localization: Nucleus
• Positive Control: HeLa or K562 cells.
• Hu-chromosome Location: 10q25.2
• Unigene: 602078 & 676603
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C; Antibody without azide: store at -20 to -80 degree C; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

FCM/FACS (Flow Cytometry)

(Flow Cytometric Analysis of PFA-fixed HeLa cells. MXI1 Mouse Monoclonal Antibody (PCRP-MXI1-1A3) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).)

Related Product Information for anti-MXI1 antibody

It is now well established that Myc regulation of cell proliferation and differentiation involves a family of related transcription factors. One such factor, Max, is an obligate heterodimeric partner for Myc and can also form heterodimers with at least four related proteins designated Mad 1, Mxi1 (also designated Mad 2), Mad 3 and Mad 4. Like Mad 1 and Mxi1, association of Mad 3 and Mad 4 with Max results in transcriptional repression. Both Myc and the Mad proteins have short half-lives and their synthesis is tightly regulated, while Max expression is constitutive and relatively stable. Two related mammalian cDNAs have been identified and shown to encode Mad- binding proteins. Both possess sequence homology with the yeast transcription repressor Sin3 including four conserved paired amphipathic helix (PAH) domains. mSin3A and mSin3B specifically interact with the Mad proteins via their second paired amphipathic helix domain (PAH2). It has been suggested that Mad-Max heterodimers repress transcription by tethering mSin3 to DNA as corepressors.

NCBI and Uniprot Product Information

• NCBI GI #: 57242784
• NCBI GeneID: 4601
• NCBI Accession #: NP_001008541.1
• NCBI GenBank Nucleotide #: NM_001008541.1
• UniProt Accession #: P50539; Q15887; Q6FHW2; Q96E53; B1ANN7; D3DR25; D3DRA9
• Molecular Weight: 26,062 Da
• NCBI Official Full Name: max-interacting protein 1 isoform c
• NCBI Official Synonym Full Names: MAX interactor 1, dimerization protein
• NCBI Official Symbol: MXI1
• NCBI Official Synonym Symbols: MXI; MAD2; MXD2; bHLHc11
• NCBI Protein Information: max-interacting protein 1; MAX dimerization protein 2; Max-related transcription factor; class C basic helix-loop-helix protein 11
• UniProt Protein Name: Max-interacting protein 1
• UniProt Gene Name: MXI1
• UniProt Synonym Gene Names: BHLHC11; Max interactor 1; bHLHc11
• UniProt Entry Name: MXI1_HUMAN