Application Data
(HT1080 lysate probed with Mouse anti Human N-cadherin (AAA50211) followed by Rabbit F(ab')2 anti Mouse IgG:HRP)
Mouse N-CADHERIN Monoclonal Antibody | anti-Cdh2 antibody
MOUSE ANTI HUMAN N-CADHERIN
Gene Names
Cdh2; N-cadherin
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation, Immunofluorescence, Immunohistochemistry
Synonyms
N-CADHERIN, Antibody; MOUSE ANTI HUMAN N-CADHERIN; anti-Cdh2 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
13A9
Form/Format
Purified
Purified IgG - liquid
Purified IgG - liquid
Concentration
IgG concentration 1.0mg/ml (varies by lot)
Sequence Length
906
Applicable Applications for anti-Cdh2 antibody
WB (Western Blot), IHC (Immunohistochemistry), IP (Immunoprecipitation), IF (Immunofluorescence), IHC (Immunohistochemistry)
Perservative Stabilisers
0.09% Sodium Azide (NaN3)
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen
Recombinant MBP fusion protein containing the entire cytoplasmic domain of human N-cadherin.
Buffer Solution
Phosphate buffered saline
Target Species
Human
Species Cross Reactivity
Rat
Reactivity Note
N.B Antibody reactivity and working conditions may vary between species. Cross reactivity is derived from testing within laboratories, peer-reviewed publications or personal communications.
Preparation and Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-80°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Application Data
(HT1080 lysate probed with Mouse anti Human N-cadherin (AAA50211) followed by Rabbit F(ab')2 anti Mouse IgG:HRP)
Application Data
(HT1080 lysate probed with Mouse anti Human N-cadherin (AAA50211) followed by Rabbit F(ab')2 anti Mouse IgG:HRP)
Related Product Information for anti-Cdh2 antibody
Mouse anti Human N-cadherin antibody, clone 13A9 specifically recognizes neural cadherin, otherwise known as CD325, a calcium dependent cell-cell adhesion glycoprotein, and member of the cadherin superfamily, which links to the actin cytoskeleton via catenins, and plays a role in cell-matrix adhesion, cell growth and differentiation, and the establishment of left-right asymmetry.
N-cadherin is expressed by neurons, endothelial cells, muscle cells, and stem cells, and is one of the primary cadherins recruited to the site of neuronal synapse formation. N-cadherin is directly involved in the differentiation of early hematopoietic progenitors, and is commonly expressed by cancer cells, playing a role in transendothelial migration and metastasis, through the up-regulation of the src kinase pathway, and subsequent failure of the intercellular connection between two adjacent endothelial cells.
Mouse anti Human N-cadherin antibody, clone 13A9 studies have demonstrated that expression levels of E-Cadherin and N-Cadherin have a role to play in the invasive properties of breast cancer. Decreased levels of E-cadherin and loss of E-cadherin-mediated adhesion, can result in the transition of a benign epithelial tumor to an invasive tumor, and increase invasiveness, whilst the expression of N-cadherin correlates with motility, invasiveness and tumor metastasis, irrespective of the presence of E-cadherin (Nieman et al. 1999). Mouse anti Human N-cadherin antibody, clone 13A9 has been shown to be specific for N-cadherin, and does not recognize E-cadherin, M-cadherin or P-cadherin (Knudsen et al. 1995). Immunohistological studies have shown that clone 13A9 can be used as a reliable marker for the differential identification of pleural mesotheliomas and lung adenocarcinomas, when used in conjunction with E-cadherin (Han et al. 1997).
N-cadherin is expressed by neurons, endothelial cells, muscle cells, and stem cells, and is one of the primary cadherins recruited to the site of neuronal synapse formation. N-cadherin is directly involved in the differentiation of early hematopoietic progenitors, and is commonly expressed by cancer cells, playing a role in transendothelial migration and metastasis, through the up-regulation of the src kinase pathway, and subsequent failure of the intercellular connection between two adjacent endothelial cells.
Mouse anti Human N-cadherin antibody, clone 13A9 studies have demonstrated that expression levels of E-Cadherin and N-Cadherin have a role to play in the invasive properties of breast cancer. Decreased levels of E-cadherin and loss of E-cadherin-mediated adhesion, can result in the transition of a benign epithelial tumor to an invasive tumor, and increase invasiveness, whilst the expression of N-cadherin correlates with motility, invasiveness and tumor metastasis, irrespective of the presence of E-cadherin (Nieman et al. 1999). Mouse anti Human N-cadherin antibody, clone 13A9 has been shown to be specific for N-cadherin, and does not recognize E-cadherin, M-cadherin or P-cadherin (Knudsen et al. 1995). Immunohistological studies have shown that clone 13A9 can be used as a reliable marker for the differential identification of pleural mesotheliomas and lung adenocarcinomas, when used in conjunction with E-cadherin (Han et al. 1997).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
cadherin-2
NCBI Official Synonym Full Names
cadherin 2
NCBI Official Symbol
Cdh2
NCBI Official Synonym Symbols
N-cadherin
NCBI Protein Information
cadherin-2; cadherin 2 type 1 N-cadherin (neuronal); cadherin 2, type 1, N-cadherin (neuronal); neural cadherin
UniProt Protein Name
Cadherin-2
UniProt Gene Name
Cdh2
UniProt Synonym Gene Names
N-cadherin
UniProt Entry Name
CADH2_RAT
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Product Notes
The Cdh2 cdh2 (Catalog #AAA50211) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's N-CADHERIN can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), IP (Immunoprecipitation), IF (Immunofluorescence), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the Cdh2 cdh2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "N-CADHERIN, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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