Mouse anti-Human NAA10/ARD1 Monoclonal Antibody | anti-NAA10 antibody

Anti-NAA10/ARD1 Antibody, Mouse Monoclonal

Cat. Num. AAA255749

• Gene Names: NAA10; TE2; ARD1; NATD; ARD1A; ARD1P; DXS707; MCOPS1
• Reactivity: Human
• Applications: Immunofluorescence, Immunocytochemistry, Flow Cytometry, Functional Assay
• Purity: Protein A
• Synonyms: NAA10/ARD1, Antibody; Anti-NAA10/ARD1 Antibody, Mouse Monoclonal; ARD1 Antibody, Mouse MAb; N(alpha)-acetyltransferase 10, NatA catalytic subunit; Anti-ARD1 Antibody; Anti-ARD1A Antibody; Anti-ARD1P Antibody; Anti-DXS707 Antibody; Anti-MCOPS1 Antibody; Anti-NAA10 Antibody; Anti-NATD Antibody; Anti-OGDNS Antibody; Anti-TE2 Antibody; anti-NAA10 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG2a
• Clone Number: 08
• Specificity: Human ARD1
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-NAA10 antibody: IF (Immunofluorescence), ICC (Immunocytochemistry), FCM/FACS (Flow Cytometry)
• Immunogen: Recombinant Human ARD1 protein
• Conjugation: Unconjugated
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human ARD1 (rh ARD1). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

FCM/FACS (Flow Cytometry)

(Flow cytometric analysis of Human ARD1A expression on HeLa cells. The cells were treated according to manufacturer's manual (BD Pharmingen'), stained with purified anti-Human ARD1A, then a PE-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)

IF (Immunofluorescence)

(Immunofluorescence staining of Human ARD1A in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with mouse anti-Human ARD1A monoclonal antibody (1:60) at 37 degree C 1 hour. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm and nucleus.)

Related Product Information for anti-NAA10 antibody

ARD1 is a member of the 2-kDa ARF protein family. It is a multifunctional protein. ARD1 has an 18-kDa ADP-ribosylation factor (ARF) domain at the C-terminus (amino acids 43-574), and a 46-kDa N-terminal domain (amino acids 1-42). The C-terminal region of ARD1 may be involved in the formation of both ARD1-ARD1 and ARD1-NAT1 complexes. ARD1 and NAT1 genes are required for the expression of an N-terminal protein acetyltransferase. This activity is required for full repression of the silent mating-type locus HML, for sporulation, and for entry into G. Recombinant ARD1 (amino acids 1-574) or its RING finger domain (amino acids 1-11) produced polyubiquitylated proteins when incubated in vitro with a mammalian E1, an E2 enzyme, ATP, and ubiquitin.

References

Tribioli C., et al., (1994), Isolation of new genes in distal Xq28: transcriptional map and identification of a human homologue of the ARD1 N-acetyl transferase of Saccharomyces cerevisiae. Hum. Mol. Genet. 3:1061-1068.Arnesen T., et al., (2005), Identification and characterization of the human ARD1-NATH protein acetyltransferase complex.Biochem. J. 386:433-443.Ross M.T., et al., (2005), The DNA sequence of the human X chromosome.Nature 434:325-337.

NCBI and Uniprot Product Information

• NCBI GI #: 728880
• NCBI GeneID: 8260
• UniProt Accession #: P41227; A6NM98
• Molecular Weight: 235
• NCBI Official Full Name: N-alpha-acetyltransferase 10
• NCBI Official Synonym Full Names: N(alpha)-acetyltransferase 10, NatA catalytic subunit
• NCBI Official Symbol: NAA10
• NCBI Official Synonym Symbols: TE2; ARD1; NATD; ARD1A; ARD1P; DXS707; MCOPS1
• NCBI Protein Information: N-alpha-acetyltransferase 10; natA catalytic subunit Naa10; ARD1 homolog A, N-acetyltransferase; N-acetyltransferase ARD1, human homolog of; N-alpha-acetyltransferase 10, NatA catalytic subunit; N-terminal acetyltransferase complex ARD1 subunit homolog A
• UniProt Protein Name: N-alpha-acetyltransferase 10
• UniProt Gene Name: NAA10
• UniProt Synonym Gene Names: ARD1; ARD1A; TE2
• UniProt Entry Name: NAA10_HUMAN