Rabbit anti-Human NAA10/ARD1 Polyclonal Antibody | anti-NAA10 antibody

Anti-NAA10/ARD1 Antibody, Rabbit Polyclonal

Cat. Num. AAA255751

• Gene Names: NAA10; TE2; ARD1; NATD; ARD1A; ARD1P; DXS707; MCOPS1
• Reactivity: Human
• Applications: Immunoprecipitation, Immunohistochemistry, ELISA, Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: NAA10/ARD1, Antibody; Anti-NAA10/ARD1 Antibody, Rabbit Polyclonal; ARD1 Antibody, Rabbit PAb, Antigen Affinity Purified; N(alpha)-acetyltransferase 10, NatA catalytic subunit; Anti-ARD1 Antibody; Anti-ARD1A Antibody; Anti-ARD1P Antibody; Anti-DXS707 Antibody; Anti-MCOPS1 Antibody; Anti-NAA10 Antibody; Anti-NATD Antibody; Anti-OGDNS Antibody; Anti-TE2 Antibody; anti-NAA10 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human ARD1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-NAA10 antibody: IP (Immunoprecipitation), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
• Immunogen: Recombinant Human ARD1 protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human ARD1 (rh ARD1). ARD1 specific IgG was purified by Human ARD1 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

WB (Western Blot)

(Anti-ARD1A rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:26 kDaObserved band size:25 kDa)

IP (Immunoprecipitation)

(ARD1A was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell LysateLane B:0.5 mg NIH-3T3 Whole Cell Lysate2 uL anti-ARD1A rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-ARD1A rabbit polyclonal antibody,at 1:500 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 33 kDaObserved band size: 26 kDa)

IHC (Immunohistochemistry)

(Immunochemical staining of human ARD1A in human spleen with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)

Related Product Information for anti-NAA10 antibody

ARD1 is a member of the 2-kDa ARF protein family. It is a multifunctional protein. ARD1 has an 18-kDa ADP-ribosylation factor (ARF) domain at the C-terminus (amino acids 43-574), and a 46-kDa N-terminal domain (amino acids 1-42). The C-terminal region of ARD1 may be involved in the formation of both ARD1-ARD1 and ARD1-NAT1 complexes. ARD1 and NAT1 genes are required for the expression of an N-terminal protein acetyltransferase. This activity is required for full repression of the silent mating-type locus HML, for sporulation, and for entry into G. Recombinant ARD1 (amino acids 1-574) or its RING finger domain (amino acids 1-11) produced polyubiquitylated proteins when incubated in vitro with a mammalian E1, an E2 enzyme, ATP, and ubiquitin.

References

Tribioli C., et al., (1994), Isolation of new genes in distal Xq28: transcriptional map and identification of a human homologue of the ARD1 N-acetyl transferase of Saccharomyces cerevisiae. Hum. Mol. Genet. 3:1061-1068.Arnesen T., et al., (2005), Identification and characterization of the human ARD1-NATH protein acetyltransferase complex.Biochem. J. 386:433-443.Ross M.T., et al., (2005), The DNA sequence of the human X chromosome.Nature 434:325-337.

NCBI and Uniprot Product Information

• NCBI GI #: 728880
• NCBI GeneID: 8260
• UniProt Accession #: P41227; A6NM98
• Molecular Weight: 235
• NCBI Official Full Name: N-alpha-acetyltransferase 10
• NCBI Official Synonym Full Names: N(alpha)-acetyltransferase 10, NatA catalytic subunit
• NCBI Official Symbol: NAA10
• NCBI Official Synonym Symbols: TE2; ARD1; NATD; ARD1A; ARD1P; DXS707; MCOPS1
• NCBI Protein Information: N-alpha-acetyltransferase 10; natA catalytic subunit Naa10; ARD1 homolog A, N-acetyltransferase; N-acetyltransferase ARD1, human homolog of; N-alpha-acetyltransferase 10, NatA catalytic subunit; N-terminal acetyltransferase complex ARD1 subunit homolog A
• UniProt Protein Name: N-alpha-acetyltransferase 10
• UniProt Gene Name: NAA10
• UniProt Synonym Gene Names: ARD1; ARD1A; TE2
• UniProt Entry Name: NAA10_HUMAN