IHC (Immunohistochemisry)
(Immunochemical staining of human DAN in human kidney with mouse monoclonal antibody (1:100, formalin-fixed paraffin embedded sections). The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).)
Mouse anti-Human NBL1 Monoclonal Antibody | anti-NBL1 antibody
Anti-NBL1 Antibody, Mouse Monoclonal
Gene Names
NBL1; NB; DAN; NO3; DAND1; D1S1733E
Reactivity
Human
Applications
Immunohistochemistry, ELISA
Purity
Protein A
Synonyms
NBL1, Antibody; Anti-NBL1 Antibody, Mouse Monoclonal; NBL1/DAND1/DAN Antibody, Mouse MAb; neuroblastoma 1, DAN family BMP antagonist; Anti-D1S1733E Antibody; Anti-DAN Antibody; Anti-DAND1 Antibody; Anti-NB Antibody; Anti-NO3 Antibody; anti-NBL1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG1
Clone Number
01
Specificity
Human NBL1/DAND1/DAN
Purity/Purification
Protein A
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-NBL1 antibody
IHC (Immunohistochemistry), ELISA
Immunogen
Recombinant Human NBL1/DAND1/DAN protein
Conjugation
Unconjugated
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human NBL1/DAND1/DAN (rh NBL1/DAND1/DAN). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IHC (Immunohistochemisry)
(Immunochemical staining of human DAN in human kidney with mouse monoclonal antibody (1:100, formalin-fixed paraffin embedded sections). The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).)
IHC (Immunohistochemisry)
(Immunochemical staining of human DAN in human kidney with mouse monoclonal antibody (1:100, formalin-fixed paraffin embedded sections). The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).)
IHC (Immunohiostchemistry)
(Immunochemical staining of human DAN in human liver with mouse monoclonal antibody (1:100, formalin-fixed paraffin embedded sections). The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).)
IHC (Immunohiostchemistry)
(Immunochemical staining of human DAN in human liver with mouse monoclonal antibody (1:100, formalin-fixed paraffin embedded sections). The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).)
IHC (Immunohistochemistry)
(Immunochemical staining of human DAN in human lung with mouse monoclonal antibody (1:100, formalin-fixed paraffin embedded sections). The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).)
IHC (Immunohistochemistry)
(Immunochemical staining of human DAN in human lung with mouse monoclonal antibody (1:100, formalin-fixed paraffin embedded sections). The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).)
Related Product Information for anti-NBL1 antibody
The Dan (Differential screening-selected gene aberrative in neuroblastoma, also known as N03) gene was first identified as the putative rat tumor suppressor gene and encodes a protein structurally related to Cerberus and Gremlin in the vertebrates. It is a founding member of the DAN family of secreted proteins, acts as an inhibitor of cell cycle progression, and is closely involved in retinoic acid-induced neuroblastoma differentiation. There are at least five mammalian protein members in the evolutionarily conserved Dan family including DAN, Gremlin/DRM, Cer1 (Cerberus-related), Dante, and PRDC (protein related to DAN and Cerberus), and share the C-terminal cystine-knot motif. As a secreted glycoprotein, DAN is a member of a class of glycoproteins shown to be secreted inhibitors of the transforming growth factor-beta (TGF-beta) and bone morphogenic protein pathways. It binds to BMPs and preventing their interactions with signaling receptor complexes, and accordingly regulates the processes of embryonic development and tissue differentiation. DAN gene product may have an important role in the regulation of the entry of cells into the S phase. Besides, the DAN gene product possesses an ability to revert phenotypes of transformed rat fibroblasts and represents a candidate tumor suppressor gene for neuroblastoma.
References
Ozaki T, et al. (1995) Overexpression of DAN gene product in normal rat fibroblasts causes a retardation of the entry into the S phase. Cancer Res. 55(4): 895-900.Nakamura Y, et al. (1997) A product of DAN, a novel candidate tumour suppressor gene, is secreted into culture medium and suppresses DNA synthesis. Eur J Cancer. 33(12): 1986-90.Ogita J, et al. (2001) Expression of the Dan gene during chicken embryonic development. Mech Dev. 109(2): 363-5.Kim AS, et al. (2003) Expression of the BMP antagonist Dan during murine forebrain development. Brain Res Dev Brain Res. 145(1): 159-62.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
23,169 Da
NCBI Official Full Name
neuroblastoma suppressor of tumorigenicity 1 isoform 2
NCBI Official Synonym Full Names
neuroblastoma 1, DAN family BMP antagonist
NCBI Official Symbol
NBL1
NCBI Official Synonym Symbols
NB; DAN; NO3; DAND1; D1S1733E
NCBI Protein Information
neuroblastoma suppressor of tumorigenicity 1
Similar Products
Product Notes
The NBL1 (Catalog #AAA254620) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-NBL1 Antibody, Mouse Monoclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NBL1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the NBL1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NBL1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.