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FCM/FACS (Flow Cytometry)
(Flow cytometry analysis of NBL1 in A549 cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).)
Mouse anti-Human NBL1 Monoclonal Antibody | anti-NBL1 antibody
NBL1 antibody
Gene Names
NBL1; NB; DAN; NO3; DAND1; D1S1733E
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Flow Cytometry, Functional Assay, Western Blot, ELISA
Purity
By protein-A affinity chromatography
Synonyms
NBL1, Antibody; NBL1 antibody; Neuroblastoma suppressor of tumorigenicity 1; D1S1733E; DAN; DAND1; NB; NO3; anti-NBL1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
AT38G8
Purity/Purification
By protein-A affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
181
Applicable Applications for anti-NBL1 antibody
IF (Immunofluorescence), ICC (Immunocytochemistry), FCM/FACS (Flow Cytometry), WB (Western Blot), ELISA
Antigen Species
Human
Immunogen
Recombinant human NBL1 (18-181aa) purified from E. coli
Clone Note
Anti-human NBL1 mAb, clone AT38G8, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human NBL1 protein.
Preparation and Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
FCM/FACS (Flow Cytometry)
(Flow cytometry analysis of NBL1 in A549 cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).)
FCM/FACS (Flow Cytometry)
(Flow cytometry analysis of NBL1 in A549 cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).)
FCM/FACS (Flow Cytometry)
(Flow cytometry analysis of NBL1 in Jurkat cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).)
FCM/FACS (Flow Cytometry)
(Flow cytometry analysis of NBL1 in Jurkat cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).)
IF (Immunofluorescence)
(ICC/IF analysis of NBL1 in A549 cells. The cell was stained with AAA48135 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)
IF (Immunofluorescence)
(ICC/IF analysis of NBL1 in A549 cells. The cell was stained with AAA48135 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)
IF (Immunofluorescence)
(CC/IF analysis of NBL1 in Jurkat cells. The cell was stained with AAA48135 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)
IF (Immunofluorescence)
(CC/IF analysis of NBL1 in Jurkat cells. The cell was stained with AAA48135 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)
WB (Western Blot)
(The cell lysate of Jurkat (30ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NBL1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
WB (Western Blot)
(The cell lysate of Jurkat (30ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NBL1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Related Product Information for anti-NBL1 antibody
NBL1 is the founding member of the evolutionarily conserved CAN (Cerberus and DAN) family of proteins, which contain a domain resembling the CTCK (C-terminal cystine knot-like) motif found in a number of signaling molecules. These proteins are secreted, and act as BMP (bone morphogenetic protein) antagonists by binding to BMPs and preventing them from interacting with their receptors. They may thus play an important role during growth and development and alternatively spliced transcript variants have been identified for this gene. Also, as a tumor suppressor gene of neuroblastoma may play an important role in preventing cells from entering the final stage (G1/S) of the transformation process. Read-through transcripts between this locus and the upstream mitochondrial inner membrane organizing system 1 have been observed.
Product Categories/Family for anti-NBL1 antibody
References
Olakowski, M., et al. (2009) Folia Histochem Cytobiol 47 (2): 249-55; Shaikhibrahim, Z., et al. (2011) Int J Mol Med 28 (4): 605-11
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
neuroblastoma suppressor of tumorigenicity 1 isoform 2
NCBI Official Synonym Full Names
neuroblastoma 1, DAN family BMP antagonist
NCBI Official Symbol
NBL1
NCBI Official Synonym Symbols
NB; DAN; NO3; DAND1; D1S1733E
NCBI Protein Information
neuroblastoma suppressor of tumorigenicity 1
UniProt Protein Name
Neuroblastoma suppressor of tumorigenicity 1
UniProt Gene Name
NBL1
UniProt Synonym Gene Names
DAN; DAND1
Similar Products
Product Notes
The NBL1 nbl1 (Catalog #AAA48135) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The NBL1 antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NBL1 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry), FCM/FACS (Flow Cytometry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the NBL1 nbl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NBL1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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