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IHC (Immunohistchemistry)
(Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1,NFkB p65 Monoclonal Antibody(5G6) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.)
Mouse NFkappaB p65 Monoclonal Antibody | anti-NFkappaB p65 antibody
NFkappaB p65 Mouse Monoclonal Antibody
Gene Names
RELA; p65; NFKB3
Reactivity
Human, Rat, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification using immunogen.
Synonyms
NFkappaB p65, Antibody; NFkappaB p65 Mouse Monoclonal Antibody; NFkB p65 Mouse Monoclonal Antibody; MGC131774; NFKB3; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3; p65; RELA; TF65; Transcription factor p65; anti-NFkappaB p65 antibody
Host
Mouse
Reactivity
Human, Rat, Mouse
Clonality
Monoclonal
Specificity
NFkB p65 Mouse monoclonal antibody detects endogenous p65 proteins.
Purity/Purification
Affinity purification using immunogen.
Form/Format
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
548
Applicable Applications for anti-NFkappaB p65 antibody
Western Blot (WB), Immunohistochemistry(IHC), Immunofluorescence (IF)
Application Notes
WB: 1:1000~1:3000
IF: 1:100
IP: 1:200
IHC: 1:50-300
IF: 1:200
IF: 1:100
IP: 1:200
IHC: 1:50-300
IF: 1:200
Target Name
NFkB p65
Preparation and Storage
Store at -20°C.
IHC (Immunohistchemistry)
(Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1,NFkB p65 Monoclonal Antibody(5G6) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.)
IHC (Immunohistchemistry)
(Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1,NFkB p65 Monoclonal Antibody(5G6) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Human-Appendix tissue. 1,NFkB p65 Monoclonal Antibody(5G6) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Human-Appendix tissue. 1,NFkB p65 Monoclonal Antibody(5G6) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.)
IF (Immunofluorescence)
(Immunofluorescence analysis of Human-appendix tissue. 1,NFkB p65 Monoclonal Antibody(5G6)(red) was diluted at 1:200(4C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)
IF (Immunofluorescence)
(Immunofluorescence analysis of Human-appendix tissue. 1,NFkB p65 Monoclonal Antibody(5G6)(red) was diluted at 1:200(4C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)
Application Data
(Input: Hela Cell Lysate 2¡¢IP product: IP dilute 1:200 Western blot analysis: primary antibody (AAA29642): 1:2,000 Secondary antibody: Goat anti-Mouse IgG, Light chain specific, 1:5,000)
Application Data
(Input: Hela Cell Lysate 2¡¢IP product: IP dilute 1:200 Western blot analysis: primary antibody (AAA29642): 1:2,000 Secondary antibody: Goat anti-Mouse IgG, Light chain specific, 1:5,000)
IF (Immunofluorescence)
(IF analysis of Hela with (AAA29642) (Left) and DAPI (Right) diluted at 1:100.)
IF (Immunofluorescence)
(IF analysis of Hela with (AAA29642) (Left) and DAPI (Right) diluted at 1:100.)
WB (Western Blot)
(Western blot analysis of 1) Hela, 2) Rat Heart Tissue, 3) Mouse Spleen Tissue, using (AAA29642) diluted at 1:2000.)
WB (Western Blot)
(Western blot analysis of 1) Hela, 2) Rat Heart Tissue, 3) Mouse Spleen Tissue, using (AAA29642) diluted at 1:2000.)
Related Product Information for anti-NFkappaB p65 antibody
NFkB p65 is ubiquitinated leading to its proteosomal degradation, which is required for termination of the NFkB response. Phosphorylation of NFkB p65 on S536 stimulates acetylation of K310 by CBP, enhancing transcriptional activity. NFkB p65 is also acetylated at K122, enhancing DNA binding and impairing the interaction with NFKBIA. The protein is deacetylated by HDAC3. Invasion of a host by a pathogen is frequently associated with the activation of NF-kB, which coordinates various aspects of immune function required for resistance to infection.
Product Categories/Family for anti-NFkappaB p65 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
65kDa
NCBI Official Full Name
transcription factor p65 isoform 2
NCBI Official Synonym Full Names
v-rel avian reticuloendotheliosis viral oncogene homolog A
NCBI Official Symbol
RELA
NCBI Official Synonym Symbols
p65; NFKB3
NCBI Protein Information
transcription factor p65
UniProt Protein Name
Transcription factor p65
UniProt Gene Name
RELA
UniProt Synonym Gene Names
NFKB3
UniProt Entry Name
TF65_HUMAN
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Product Notes
The NFkappaB p65 rela (Catalog #AAA29642) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The NFkappaB p65 Mouse Monoclonal Antibody reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's NFkappaB p65 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry(IHC), Immunofluorescence (IF). WB: 1:1000~1:3000 IF: 1:100 IP: 1:200 IHC: 1:50-300 IF: 1:200. Researchers should empirically determine the suitability of the NFkappaB p65 rela for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NFkappaB p65, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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