FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing HepG2 cells stained with AAA279045 (red line) at 1:50. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
Mouse anti-Human NOS2 Monoclonal Antibody | anti-NOS2 antibody
Mouse anti-Homo sapiens (Human) NOS2 Monoclonal antibody
Gene Names
NOS2; NOS; INOS; NOS2A; HEP-NOS
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Western Blot, ELISA
Purity
>95%, Protein G purified
Synonyms
NOS2, Antibody; Mouse anti-Homo sapiens (Human) NOS2 Monoclonal antibody; NOS2 Monoclonal Antibody; HEP-NOS; Hepatocyte NOS; HEPNOS; inducible; Inducible nitric oxide synthase; Inducible NO synthase; Inducible NOS; iNOS; MAC NOS; Macrophage NOS; Nitric oxide synthase 2 inducible; Nitric oxide synthase 2 inducible macrophage; nitric oxide synthase 2A (inducible; hepatocytes); Nitric oxide synthase; Nitric oxide synthase inducible; nitric oxide synthase; macrophage; NOS 2; NOS; Nos II; NOS type II; nos2; NOS2_HUMAN; NOS2A; Inducible; Hepatocyte; Peptidyl-cysteine S-nitrosylase NOS2; anti-NOS2 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
2H6B10
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid; PBS, 0.1% sodium azide, 50% glycerol, pH 7.3.
Applicable Applications for anti-NOS2 antibody
FCM/FACS (Flow Cytometry), WB (Western Blot), ELISA
Immunogen Species
Human
Immunogen
Recombinant Human Nitric oxide synthase, inducible protein (1-200AA)
Conjugation
Non-conjugated
Research Area
Signal Transduction
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing HepG2 cells stained with AAA279045 (red line) at 1:50. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing HepG2 cells stained with AAA279045 (red line) at 1:50. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing Hela cells stained with AAA279045 (red line) at 1:50. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing Hela cells stained with AAA279045 (red line) at 1:50. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
WB (Western Blot)
(Western BlotPositive WB detected in: Hela whole cell lysateAll lanes: NOS2 antibody at 1:2000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 131, 127 kDaObserved band size: 131 KDaExposure time?5min)
WB (Western Blot)
(Western BlotPositive WB detected in: Hela whole cell lysateAll lanes: NOS2 antibody at 1:2000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 131, 127 kDaObserved band size: 131 KDaExposure time?5min)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
131,117 Da
NCBI Official Full Name
nitric oxide synthase, inducible
NCBI Official Synonym Full Names
nitric oxide synthase 2, inducible
NCBI Official Symbol
NOS2
NCBI Official Synonym Symbols
NOS; INOS; NOS2A; HEP-NOS
NCBI Protein Information
nitric oxide synthase, inducible; NOS type II; NOS, type II; inducible NOS; hepatocyte NOS; inducible NO synthase; nitric oxide synthase, macrophage; peptidyl-cysteine S-nitrosylase NOS2; nitric oxide synthase 2A (inducible, hepatocytes)
UniProt Protein Name
Nitric oxide synthase, inducible
UniProt Gene Name
NOS2
UniProt Synonym Gene Names
NOS2A; HEP-NOS; Inducible NOS; iNOS
UniProt Entry Name
NOS2_HUMAN
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Product Notes
The NOS2 nos2 (Catalog #AAA279045) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti-Homo sapiens (Human) NOS2 Monoclonal antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NOS2 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the NOS2 nos2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NOS2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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