Hamster anti-Rat NOTCH 2 Monoclonal Antibody | anti-NOTCH 2 antibody

HAMSTER ANTI MOUSE NOTCH 2

Cat. Num. AAA49795

• Gene Names: Notch2; N2; AI853703
• Reactivity: Rat
• Applications: Flow Cytometry, Functional Assay
• Synonyms: NOTCH 2, Antibody; HAMSTER ANTI MOUSE NOTCH 2; anti-NOTCH 2 antibody

• Host: Hamster
• Reactivity: Rat
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: HMN2-35
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 2473

• Applicable Applications for anti-NOTCH 2 antibody: FCM/FACS (Flow Cytometry)
• Perservative Stabilisers: 0.09% Sodium Azide (NaN3)
• Preparation: Purified IgG prepared by affinity chromatography on Protein G
• Reactivity Note: N.B. Antibody reactivity and working conditions may vary between species. Cross reactivity is derived from testing within our laboratories , peer reviewed publications or personal communications from the originators.
• Immunogen: Mouse Notch 2-Fc fusion protein.
• Fusion Partners: Spleen cells from immunised Armenian hamsters were fused with cells of the P3U1 myeloma cell line.
• Buffer Solution: Phosphate buffered saline
• Target Species: Mouse
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Application Data

(Mouse Notch 2 transfected cells stained with Hamster anti Mouse Notch 2)

Related Product Information for anti-NOTCH 2 antibody

Hamster anti Mouse Notch 2 antibody, clone HMN2-35 specifically recognizes Notch 2, one of the four major transmembrane receptors (Notch 1-4) of the Notch signaling pathway, which is activated through binding to DSL domain-containing membrane-bound specific ligands. The Notch signaling pathway is an evolutionarily conserved pathway in multi-cellular organisms, which is vital for cell-cell communication, important during fundamental developmental and physiological processes, including regulation of cell fate decisions during neuronal, cardiac and endocrine development, stem cell hematopoiesis, thymic T-cell development, and both tumor progression and suppression. Ligation of Notch receptors by their specific ligands, Jagged1 (CD339), Jagged2, Delta-like protein 1 (DLL1), DLL3 and DLL4, on physically adjacent signal receiving cells, induces proteolysis of the receptors by ADAM-family metalloproteases and the gamma-secretase complex, within the transmembrane domain, releasing the Notch intracellular domain (NICD) to translocate to the nucleus. Subsequent signal transduction then occurs through either the CSL-NICD-Mastermind complex cascade (canonical pathway), or NF-kappaB-NICD and CSL-NICD-Deltex complex signaling cascades (non-canonical pathway). The canonical pathway inhibits the differentiation of stem cells or progenitor cells, whilst the non-canonical pathway promotes differentiation. Signaling through Notch 2 has been implicated in the development of marginal zone B cells (MZB), the sensitization of endothelial cells to apoptosis, and the regulation of the expression of CD23 in B-cell lymphocytic leukemia (B-CLL). Studies have also shown a correlation between a decrease in Notch 2 expression and an increase in grade of human breast cancer. Hamster anti Mouse Notch 2 antibody, clone HMN2-35 has been shown to cross-react with rat mast cell line RBL-2H3 and Y3 myeloma cells, in flow cytometry.

NCBI and Uniprot Product Information

• NCBI GI #: 134288853
• NCBI GeneID: 18129
• NCBI Accession #: NP_035058.2
• NCBI GenBank Nucleotide #: NM_010928.2
• Molecular Weight: 242,663 Da
• NCBI Official Full Name: neurogenic locus notch homolog protein 2
• NCBI Official Synonym Full Names: notch 2
• NCBI Official Symbol: Notch2
• NCBI Official Synonym Symbols: N2; AI853703
• NCBI Protein Information: neurogenic locus notch homolog protein 2; Motch B; Notch gene homolog 2
• UniProt Protein Name: Neurogenic locus notch homolog protein 2
• UniProt Gene Name: Notch2
• UniProt Synonym Gene Names: Notch 2
• UniProt Entry Name: NOTC2_MOUSE