FCM/FACS (Flow Cytometry)
(Flow Cytometric analysis using Notch 4 antibodyStaining of CD8+CD11c+ C57BL/6 splenocytes with 0.25 ug of Armenian Hamster IgG Isotype Control (PE) (blue histogram) or 0.5 ug of Notch 4 antibody (PE) (purple histogram). Total viable cells were used for analysis.)
Hamster Notch 4 Monoclonal Antibody
Notch 4 antibody (PE)
Applications
Flow Cytometry, Functional Assay
Synonyms
Notch 4, Antibody; Notch 4 antibody (PE); Monoclonal Notch 4; Anti-Notch 4; Notch 4; Notch-4; Neurogenic locus notch homolog protein 4; oncogene INT3; anti-Notch 4 antibody
Host
Hamster
Clonality
Monoclonal
Isotype
IgG
Clone Number
HMN4-14
Form/Format
Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
Concentration
200 ug/ml (varies by lot)
Applicable Applications for anti-Notch 4 antibody
FCM/FACS (Flow Cytometry)
Biological Significance
This HMN4-14 monoclonal antibody reacts with mouse Notch 4, one of four members of the Notch family of receptors. Notch receptors are 300-kDa single-pass transmembrane proteins. While the extracellular domain contains numerous epidermal growth factor-like repeats for ligand binding, the intracellular domain is involved in cell signaling. Upon binding its membrane-bound ligand, the Notch receptor undergoes proteolytic cleavage, first by ADAM-family metalloproteases and then by gamma-secretase. The second cleavage event releases the Notch intracellular domain, which subsequently translocates into the nucleus, forms a ternary complex with the DNA-binding transcription factor CSL and the coactivator Mastermind, and ultimately activates transcription. Notch 4 has been shown to be expressed in CD8+ splenic dendritic cells, endothelial cells, and macrophages. A truncated, constitutively active form of Notch 4 designated Int3 also exists in mice as a result of the mouse mammary tumor virus insertion into the Notch 4 gene. Composed of the transmembrane and intracellular domains of Notch 4, Int3 has been reported to be involved in mammary gland development and tumorigenesis in the mouse. Finally, studies have shown Notch 4 involvement in myeloid proliferation, hematopoiesis, and embryonic endothelial development.
Tag/Conjugate
PE
Preparation and Storage
Store at 2-8 degree C. Do not freeze. Protect from light.
FCM/FACS (Flow Cytometry)
(Flow Cytometric analysis using Notch 4 antibodyStaining of CD8+CD11c+ C57BL/6 splenocytes with 0.25 ug of Armenian Hamster IgG Isotype Control (PE) (blue histogram) or 0.5 ug of Notch 4 antibody (PE) (purple histogram). Total viable cells were used for analysis.)
FCM/FACS (Flow Cytometry)
(Flow Cytometric analysis using Notch 4 antibodyStaining of CD8+CD11c+ C57BL/6 splenocytes with 0.25 ug of Armenian Hamster IgG Isotype Control (PE) (blue histogram) or 0.5 ug of Notch 4 antibody (PE) (purple histogram). Total viable cells were used for analysis.)
FCM/FACS (Flow Cytometry)
(Flow Cytometric analysis using Notch 4 antibodyStaining of CD8+CD11c+ C57BL/6 splenocytes with 0.25 ug of Armenian Hamster IgG Isotype Control (PE) (blue histogram) or 0.5 ug of Notch 4 antibody (PE) (purple histogram). Total viable cells were used for analysis.)
FCM/FACS (Flow Cytometry)
(Flow Cytometric analysis using Notch 4 antibodyStaining of CD8+CD11c+ C57BL/6 splenocytes with 0.25 ug of Armenian Hamster IgG Isotype Control (PE) (blue histogram) or 0.5 ug of Notch 4 antibody (PE) (purple histogram). Total viable cells were used for analysis.)
Related Product Information for anti-Notch 4 antibody
Armenian Hamster monoclonal Notch4 antibody (PE)
Product Categories/Family for anti-Notch 4 antibody
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Product Notes
The Notch 4 (Catalog #AAA106811) is an Antibody produced from Hamster and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Notch 4 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the Notch 4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Notch 4, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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