Mouse p53 Monoclonal Antibody | anti-p53 antibody

MOUSE ANTI p53 (aa20-25)

Cat. Num. AAA49286

• Gene Names: TP53; P53; BCC7; LFS1; TRP53
• Reactivity: Bovine; Does not react with Mouse, and Rat
• Applications: Western Blot, Immunohistochemistry, Immunoprecipitation, Flow Cytometry, Functional Assay, Immunohistochemistry
• Synonyms: p53, Antibody; MOUSE ANTI p53 (aa20-25); anti-p53 antibody

• Host: Mouse
• Reactivity: Bovine; Does not react with Mouse, and Rat
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: DO-7
• Specificity: p53 (aa20-25)
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0 mg/ml (varies by lot)
• Sequence Length: 214

• Applicable Applications for anti-p53 antibody: WB (Western Blot), IHC (Immunohistochemistry), IP (Immunoprecipitation), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry)
• Perservative Stabilisers: 0.09% Sodium Azide
• Preparation: Purified IgG prepared by affinity chromatography on Protein G
• Immunogen: Recombinant Human p53.
• Histology Positive Control Tissue: Colon or breast carcinoma; Buffer Solution
• Target Species: Human
• Reactivity Note: N.B. Antibody reactivity and working conditions may vary between species. Cross reactivity is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information.
• Fusion Partners: Spleen cells from immunized Balb/c mice fused with cells of the mouse X63Ag8.653 myeloma cell lines.
• Preparation and Storage: It is recommended to aliquot and store at -20°C on receipt. When thawed , aliquot the sample as needed. Keep aliquots at 2°C to 8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.; Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

Application Data

(Formalin fixed, paraffin processed human breast carcinoma stained with Mouse anti p53)

Application Data

(Western blot analysis of HEK293 cell lysate probed with Mouse anti p53 (aa20-25) followed by HRP conjugated Goat anti Mouse IgG, visualized using chemiluminescence)

Related Product Information for anti-p53 antibody

Mouse anti p53 antibody, clone DO-7 recognizes the human 53kDa p53 tumour suppressor protein, also known as Cellular tumor antigen p53 or Antigen NY-CO-13, encoded by the TP53 gene. p53 is a 393 amino acid protein with an N-terminal transactivation domain, followed by a proline-rich region and a DNA binding domain in the central core region. The C-terminal region contains a tetramirization domain and a terminal regulatory domain (Joerger et al. 2010, Bai et al. 2006). p53 is intimately involved in a number of signaling pathways controlling cell division, cycling and apoptosis (Haupt et al. 2003) and is thus a potent cancer suppressor. In normal cells the level of p53 expression is low but can be induced by DNA damage or other stress signals (Takagi et al. 2005). Activation of p53 leads to growth arrest through its interaction with p21, GADD45 and 14-3-3σ, DNA repair and potentially apoptosis through interaction with Bax, Apaf-1, PUMA and NoxA (Thakur et al. 2010). p53 is critically regulated by Mdm2 which can trigger p53 degradation by a ubiquitin dependent system (Moll and Petrenko 2003) Mouse anti p53 antibody, clone DO-7 recognizes an epitope at the N-terminal end of p53 between amino acids 20-25, binding to both wild type and mutant forms. Clone DO-7 is not expected to recognize the multiple isoforms lacking the N-teminal region.

NCBI and Uniprot Product Information

• NCBI GI #: 120407068
• NCBI GeneID: 7157
• NCBI Accession #: NP_000537.3
• NCBI GenBank Nucleotide #: NM_000546.5
• UniProt Accession #: P04637; Q15086; Q15087; Q15088; Q16535; Q16807; Q16808; Q16809; Q16810; Q16811; Q16848; Q2XN98
• Molecular Weight: 24,401 Da
• NCBI Official Full Name: cellular tumor antigen p53 isoform a
• NCBI Official Synonym Full Names: tumor protein p53
• NCBI Official Symbol: TP53
• NCBI Official Synonym Symbols: P53; BCC7; LFS1; TRP53
• NCBI Protein Information: cellular tumor antigen p53; antigen NY-CO-13; mutant tumor protein 53; p53 tumor suppressor; phosphoprotein p53; transformation-related protein 53
• UniProt Protein Name: Cellular tumor antigen p53
• UniProt Gene Name: TP53
• UniProt Synonym Gene Names: P53
• UniProt Entry Name: P53_HUMAN