Rabbit anti-Human p53 Monoclonal Antibody | anti-TP53 antibody

[KO Validated] p53 Rabbit mAb

Cat. Num. AAA282751

• Reactivity: Human
• Applications: ELISA, Immunoprecipitation, Western Blot
• Purity: Affinity purification
• Synonyms: p53, Antibody; [KO Validated] p53 Rabbit mAb; P53; BCC7; LFS1; BMFS5; TRP53; 53; anti-TP53 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Purity/Purification: Affinity purification
• Form/Format: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
• Sequence: MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD

• Applicable Applications for anti-TP53 antibody: ELISA, IP (Immunoprecipitation), WB (Western Blot)
• Cross Reactivity: Human, Rat
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-393 of human p53 (NP_000537.3).
• Preparation and Storage: Store at -20 degree C. Avoid freeze / thaw cycles.|Buffer: PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3.

IP (Immunoprecipitation)

(Immunoprecipitation analysis of 300 ug extracts from 293T cells using 3 ug [KO Validated] p53 Rabbit mAb (AAA282751). Western blot was performed from the immunoprecipitate using [KO Validated] p53 Rabbit mAb (AAA282751) at a dilution of 1:1000.)

WB (Western Blot)

(Western blot analysis of lysates from wild type (WT) and p53 knockout (KO) 293T cells using [KO Validated] p53 Rabbit mAb (AAA282751) at 1:59000 dilution incubated overnight at 4?.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 60s.)

Related Product Information for anti-TP53 antibody

This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277).

Product Categories/Family for anti-TP53 antibody

Epigenetics Nuclear Signaling; Transcription Factors; DNA Damage Repair; Protein phosphorylation; Cancer; Tumor suppressors; p53 pathway; Signal Transduction; PI3K-Akt Signaling Pathway; ErbB-HER Signaling Pathway; MAPK-JNK Signaling Pathway; ATM Signalin

NCBI and Uniprot Product Information

• NCBI GI #: 120407068
• NCBI GeneID: 7157
• NCBI Accession #: NP_000537.3
• NCBI GenBank Nucleotide #: NM_000546.5
• UniProt Accession #: P04637; Q15086; Q15087; Q15088; Q16535; Q16807; Q16808; Q16809; Q16810; Q16811; Q16848; Q2XN98
• Molecular Weight: Calculated MW: 44kDa; Observed MW: 53kDa
• UniProt Protein Name: Cellular tumor antigen p53
• UniProt Gene Name: TP53
• UniProt Synonym Gene Names: P53
• UniProt Entry Name: P53_HUMAN