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ICC (Immunocytochemistry)
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Rabbit p54nrb Monoclonal Antibody | anti-p54nrb antibody

p54nrb Antibody

Gene Names
NONO; P54; NMT55; NRB54; MRXS34; P54NRB; PPP1R114
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
Purity
ProA affinity purified
Synonyms
p54nrb, Antibody; p54nrb Antibody; 52 kDa subunit antibody; 54 kDa nuclear RNA and DNA binding protein antibody; 54 kDa nuclear RNA- and DNA-binding protein antibody; 55 kDa nuclear protein antibody; DNA binding p52/p100 complex 52 kDa subunit antibody; DNA-binding p52/p100 complex antibody; NMT 55 antibody; NMT55 antibody; Non Pou domain containing octamer (ATGCAAAT) binding protein antibody; Non POU domain containing octamer binding antibody; Non POU domain containing octamer binding protein antibody; Non-POU domain-containing octamer-binding protein antibody; Nono antibody; NonO protein antibody; NONO_HUMAN antibody; NRB 54 antibody; NRB antibody; NRB54 antibody; Nuclear RNA binding protein 54kD antibody; P54 antibody; p54 (nrb) antibody; p54nrb antibody; PPP1R114 antibody; Protein phosphatase 1 regulatory subunit 114 antibody; anti-p54nrb antibody
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For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Clone Number
JG87-38
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-p54nrb antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF),Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC: 1:50
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein of C-terminal human p54nrb.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.

ICC (Immunocytochemistry)

(ICC staining p54nrb in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistchemistry)

(Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti- p54nrb antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-p54nrb antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded human colon tissue using anti- p54nrb antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded human liver tissue using anti- p54nrb antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti- p54nrb antibody. Counter stained with hematoxylin.)

WB (Western Blot)

(Western blot analysis of p54nrb on 293 (1) and SH-SY-5Y (2) cell lysate using anti-p54nrb antibody at 1/500 dilution.)

Related Product Information for anti-p54nrb antibody
Found in both primary and transformed human cells, paraspeckles are discrete bodies in the interchromatin nucleoplasmic space which contain p54/nrb (nuclear RNA-binding protein) and at least two other RNA-binding proteins, paraspeckle protein 1 (PSP1) and paraspeckle protein 2 (PSP2). Paraspeckles often co-localize with splicing speckles, which are the site of splicing factor accumulation. Paraspeckle proteins, including p54/nrb, move dynamically between the nucleolus and paraspeckles and translocate to distinct caps in the nucleolar periphery when transcription is inhibited. Originally purified from HeLa cells, the nuclear p54/nrb has two RNA recognition motifs and shares extensive homology with both the human splicing factor PSF and Drosophila NONA/BJ6, which is required for normal vision and courtship. The shared domain between these proteins is termed a DBHS (Drosophila behavior, human splicing) domain and may play a role in regulating various pathways at the level of pre-mRNA splicing.
Product Categories/Family for anti-p54nrb antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
43,866 Da
NCBI Official Full Name
non-POU domain-containing octamer-binding protein isoform 1
NCBI Official Synonym Full Names
non-POU domain containing, octamer-binding
NCBI Official Symbol
NONO
NCBI Official Synonym Symbols
P54; NMT55; NRB54; MRXS34; P54NRB; PPP1R114
NCBI Protein Information
non-POU domain-containing octamer-binding protein
UniProt Protein Name
Non-POU domain-containing octamer-binding protein
UniProt Gene Name
NONO
UniProt Synonym Gene Names
NRB54; NonO protein; p54nrb
UniProt Entry Name
NONO_HUMAN

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Product Notes

The p54nrb nono (Catalog #AAA30509) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The p54nrb Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's p54nrb can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF),Immunohistochemistry (IHC). WB: 1:500-1:2000 IHC: 1:50-1:200 ICC: 1:50. Researchers should empirically determine the suitability of the p54nrb nono for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "p54nrb, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.