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ICC (Immunocytochemistry)
(ICC staining PDPK1 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Rabbit PDPK1 Monoclonal Antibody | anti-PDPK1 antibody
PDPK1 Antibody
Gene Names
PDPK1; PDK1; PDPK2; PRO0461
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
Purity
ProA affinity purified
Synonyms
PDPK1, Antibody; PDPK1 Antibody; 3 phosphoinositide dependent protein kinase 1 antibody; 3-phosphoinositide-dependent protein kinase 1 antibody; hPDK 1 antibody; hPDK1 antibody; MGC20087 antibody; MGC35290 antibody; OTTHUMP00000159109 antibody; OTTHUMP00000159110 antibody; OTTHUMP00000174525 antibody; PDK1 antibody; Pdpk1 antibody; PDPK1_HUMAN antibody; PDPK2 antibody; PDPK2P antibody; PkB kinase antibody; PkB kinase like gene 1 antibody; PkB like 1 antibody; PRO0461 antibody; Protein kinase antibody; anti-PDPK1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Clone Number
SD204-9
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-PDPK1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC)
Application Notes
WB: 1:1000-1:2000
IHC: 1:50-1:200
ICC: 1:100-1:500
IHC: 1:50-1:200
ICC: 1:100-1:500
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
ICC (Immunocytochemistry)
(ICC staining PDPK1 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining PDPK1 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining PDPK1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining PDPK1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining PDPK1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining PDPK1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PDPK1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PDPK1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-PDPK1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-PDPK1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-PDPK1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-PDPK1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-PDPK1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-PDPK1 antibody. Counter stained with hematoxylin.)
WB (Western Blot)
(Western blot analysis of PDPK1 on different lysates using anti-PDPK1 antibody at 1/1, 000 dilution. Positive control: Lane 1: 293T Lane 2: PC-12 Lane 3: HL-60)
WB (Western Blot)
(Western blot analysis of PDPK1 on different lysates using anti-PDPK1 antibody at 1/1, 000 dilution. Positive control: Lane 1: 293T Lane 2: PC-12 Lane 3: HL-60)
Related Product Information for anti-PDPK1 antibody
PDPK1 (3-phosphoinositide dependent protein kinase 1), also known as PDK1, PDPK2, PDPK2P or PRO0461, is 556 amino acid ubiquitously expressed protein that localizes to the cell membrane, cytoplasm and nucleus. Acting as a master kinase, PDPK1 phosphorylates and activates a subgroup of the AGC family of protein kinases. PDPK1 is involved in mediating signal transduction for controlling proliferation, survival, and growth of developing pancreatic beta cells, regulating Ca2+ uptake and Ca2+-activated K+ channels of mast cells, regulation of chemotaxis and motility of vascular endothelial cells, cardiac homeostasis, and thymocyte development. Belonging to the protein kinase superfamily, PDPK1 contains a PH domain, which play an essential role in homodimerization, localization and nuclear import of PDPK1, and a protein kinase domain. PDPK1 exists as five alternatively spliced isoforms and is encoded by a gene located on human chromosome 16p13.3.
Product Categories/Family for anti-PDPK1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
63,152 Da
NCBI Official Full Name
3-phosphoinositide-dependent protein kinase 1 isoform 3
NCBI Official Synonym Full Names
3-phosphoinositide dependent protein kinase-1
NCBI Official Symbol
PDPK1
NCBI Official Synonym Symbols
PDK1; PDPK2; PRO0461
NCBI Protein Information
3-phosphoinositide-dependent protein kinase 1; PkB-like 1; PkB kinase like gene 1
UniProt Protein Name
3-phosphoinositide-dependent protein kinase 1
UniProt Gene Name
PDPK1
UniProt Synonym Gene Names
PDK1; hPDK1
UniProt Entry Name
PDPK1_HUMAN
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Product Notes
The PDPK1 pdpk1 (Catalog #AAA30194) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PDPK1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PDPK1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC). WB: 1:1000-1:2000 IHC: 1:50-1:200 ICC: 1:100-1:500. Researchers should empirically determine the suitability of the PDPK1 pdpk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PDPK1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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