Mouse anti-Human PER2 Monoclonal Antibody | anti-PER2 antibody

Anti-PER2 Antibody (clone 5C10)

Cat. Num. AAA162235

• Gene Names: PER2; FASPS; FASPS1
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: Purified from ascites by Protein A
• Synonyms: PER2, Antibody; Anti-PER2 Antibody (clone 5C10); Rabbit Polyclonal to Human PER2; PER2; HPER2; Period homolog 2 (Drosophila); Period 2; KIAA0347; Period (Drosophila) homolog 2; Period circadian clock 2; Period circadian protein 2; FASPS; anti-PER2 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b, k
• Clone Number: 5C10
• Purity/Purification: Purified from ascites by Protein A
• Form/Format: PBS, pH 7.4
• Concentration: 0.4mg/mL (varies by lot)
• Sequence Length: 1255

• Applicable Applications for anti-PER2 antibody: WB (Western Blot), IHC (Immunohistochemistry), ELISA
• Immunogen Species: PER2 antibody was raised against Human
• Immunogen: PER2 (NP_073728, 1 a.a. ~ 101 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Antigen Type: Recombinant protein
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and AAA Biotech makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Store at -20 degree C.; Aliquot to avoid freeze/thaw cycles.

ELISA

(Detection limit for recombinant GST tagged PER2 is approximately 0.03 ng/ml as a capture antibody.)

IHC (Immunohistochemistry)

(Anti-PER2 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

NCBI and Uniprot Product Information

• NCBI GI #: 12707562
• NCBI GeneID: 8864
• NCBI Accession #: NP_073728.1
• NCBI GenBank Nucleotide #: NM_022817.2
• UniProt Accession #: O15055; Q4ZG49; Q6DT41; Q9UQ45; A2I2P7
• NCBI Official Full Name: period circadian protein homolog 2
• NCBI Official Synonym Full Names: period circadian regulator 2
• NCBI Official Symbol: PER2
• NCBI Official Synonym Symbols: FASPS; FASPS1
• NCBI Protein Information: period circadian protein homolog 2
• UniProt Protein Name: Period circadian protein homolog 2
• UniProt Gene Name: PER2
• UniProt Synonym Gene Names: KIAA0347; hPER2