IF (Immunofluorescence)
(Immunofluorescence analysis of PC-12 cells using Phospho-POLR2A CTD-S2 Rabbit mAb (AAA28612) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.)
Rabbit anti-Human POLR2A CTD-S2 Monoclonal Antibody | anti-POLR2A antibody
Phospho-POLR2A CTD-S2 Rabbit mAb
IHC-P: 1:50-1:200
IF/ICC: 1:50-1:200
ELISA: Recommended starting concentration is 1ug/mL.
Please optimize the concentration based on your specific assay requirements.
ChIP: 5ug antibody for 10ug-15ug of Chromatin
ChIP-seq: 1:50-1:100
CUT&Tag: 10? cells /1 ug
IF (Immunofluorescence)
(Immunofluorescence analysis of PC-12 cells using Phospho-POLR2A CTD-S2 Rabbit mAb (AAA28612) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of PC-12 cells using Phospho-POLR2A CTD-S2 Rabbit mAb (AAA28612) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of NIH/3T3 cells using Phospho-POLR2A CTD-S2 Rabbit mAb (AAA28612) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of NIH/3T3 cells using Phospho-POLR2A CTD-S2 Rabbit mAb (AAA28612) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of HeLa cells using Phospho-POLR2A CTD-S2 Rabbit mAb (AAA28612) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of HeLa cells using Phospho-POLR2A CTD-S2 Rabbit mAb (AAA28612) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of A-549 cells using Phospho-POLR2A CTD-S2 Rabbit mAb (AAA28612) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of A-549 cells using Phospho-POLR2A CTD-S2 Rabbit mAb (AAA28612) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.)
IHC (Immunohistchemistry)
(Immunohistochemistry analysis of paraffin-embedded Rat kidney using Phospho-POLR2A CTD-S2 Rabbit mAb (AAA28612) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
IHC (Immunohistchemistry)
(Immunohistochemistry analysis of paraffin-embedded Rat kidney using Phospho-POLR2A CTD-S2 Rabbit mAb (AAA28612) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
IHC (Immunohistochemistry)
(Immunohistochemistry analysis of paraffin-embedded Mouse colon using Phospho-POLR2A CTD-S2 Rabbit mAb (AAA28612) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
IHC (Immunohistochemistry)
(Immunohistochemistry analysis of paraffin-embedded Mouse colon using Phospho-POLR2A CTD-S2 Rabbit mAb (AAA28612) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
IHC (Immunohistochemistry)
(Immunohistochemistry analysis of paraffin-embedded Human cervix cancer using Phospho-POLR2A CTD-S2 Rabbit mAb (AAA28612) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
IHC (Immunohistochemistry)
(Immunohistochemistry analysis of paraffin-embedded Human cervix cancer using Phospho-POLR2A CTD-S2 Rabbit mAb (AAA28612) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
WB (Western Blot)
(Western blot analysis of lysates from C6 cells, using Phospho-POLR2A CTD-S2 Rabbit mAb (AAA28612) at 1:1000 dilution. C6 cells were treated by CIP(20uL/400ul) at 37? for 1 hour.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (RM00020).Exposure time: 1min.)
WB (Western Blot)
(Western blot analysis of lysates from C6 cells, using Phospho-POLR2A CTD-S2 Rabbit mAb (AAA28612) at 1:1000 dilution. C6 cells were treated by CIP(20uL/400ul) at 37? for 1 hour.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (RM00020).Exposure time: 1min.)
WB (Western Blot)
(Western blot analysis of various lysates using Phospho-POLR2A CTD-S2 Rabbit mAb (AAA28612) at 1:1000 dilution. MCF7 cells and HeLa cells and C2C12 cells were treated by CIP(20uL/400ul) at 37? for 1 hour.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (RM00020).Exposure time: 10s.)
WB (Western Blot)
(Western blot analysis of various lysates using Phospho-POLR2A CTD-S2 Rabbit mAb (AAA28612) at 1:1000 dilution. MCF7 cells and HeLa cells and C2C12 cells were treated by CIP(20uL/400ul) at 37? for 1 hour.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (RM00020).Exposure time: 10s.)
Application Data
(CUT&Tag was performed using the CUT&Tag Assay Kit(pAG-Tn5) forIllumina (RK20265) from 10? Hela cells with 1ug Phospho-POLR2A CTD-S2 Rabbit mAb(AAA28612), along with a Goat Anti-Rabbit IgG(H+L). The CUT&Tag results indicate the enrichment pattern of Phospho-POLR2A CTD-S2 in representative gene loci(GAPDH).)
Application Data
(CUT&Tag was performed using the CUT&Tag Assay Kit(pAG-Tn5) forIllumina (RK20265) from 10? Hela cells with 1ug Phospho-POLR2A CTD-S2 Rabbit mAb(AAA28612), along with a Goat Anti-Rabbit IgG(H+L). The CUT&Tag results indicate the enrichment pattern of Phospho-POLR2A CTD-S2 in representative gene loci(GAPDH).)
Application Data
(CUT&Tag was performed using the CUT&Tag Assay Kit(pAG-Tn5) forIllumina (RK20265) from 10? Hela cells with 1ug Phospho-POLR2A CTD-S2 Rabbit mAb(AAA28612), along with a Goat Anti-Rabbit IgG(H+L). The CUT&Tag results indicate the enrichment pattern of Phospho-POLR2A CTD-S2 in representative gene loci(GAPDH).)
Application Data
(CUT&Tag was performed using the CUT&Tag Assay Kit(pAG-Tn5) forIllumina (RK20265) from 10? Hela cells with 1ug Phospho-POLR2A CTD-S2 Rabbit mAb(AAA28612), along with a Goat Anti-Rabbit IgG(H+L). The CUT&Tag results indicate the enrichment pattern of Phospho-POLR2A CTD-S2 in representative gene loci(GAPDH).)
ChIP (Chromatin Immunoprecipitation)
(Chromatin immunoprecipitations were performed with cross-linked chromatin from 293F cells and Phospho-POLR2A-S2 (AAA28612). The ChIP sequencing results indicate the enrichment pattern of Phospho-POLR2A-S2 in selected genomic region and representative gene loci (GAPDH), as shown in figure.)
ChIP (Chromatin Immunoprecipitation)
(Chromatin immunoprecipitations were performed with cross-linked chromatin from 293F cells and Phospho-POLR2A-S2 (AAA28612). The ChIP sequencing results indicate the enrichment pattern of Phospho-POLR2A-S2 in selected genomic region and representative gene loci (GAPDH), as shown in figure.)
ChIP (Chromatin Immunoprecipitation)
(Chromatin immunoprecipitations were performed with cross-linked chromatin from 293F cells and Phospho-POLR2A-S2 (AAA28612). The ChIP sequencing results indicate the enrichment pattern of Phospho-POLR2A-S2 in selected genomic region and representative gene loci (GAPDH), as shown in figure.)
ChIP (Chromatin Immunoprecipitation)
(Chromatin immunoprecipitations were performed with cross-linked chromatin from 293F cells and Phospho-POLR2A-S2 (AAA28612). The ChIP sequencing results indicate the enrichment pattern of Phospho-POLR2A-S2 in selected genomic region and representative gene loci (GAPDH), as shown in figure.)
NCBI and Uniprot Product Information
Observed MW: 270kDa
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Product Notes
The POLR2A polr2a (Catalog #AAA28612) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-POLR2A CTD-S2 Rabbit mAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's POLR2A CTD-S2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA), Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation (ChIP)-seq, CUT&Tag. WB: 1:500-1:2000 IHC-P: 1:50-1:200 IF/ICC: 1:50-1:200 ELISA: Recommended starting concentration is 1ug/mL. Please optimize the concentration based on your specific assay requirements. ChIP: 5ug antibody for 10ug-15ug of Chromatin ChIP-seq: 1:50-1:100 CUT&Tag: 10? cells /1 ug. Researchers should empirically determine the suitability of the POLR2A polr2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "POLR2A CTD-S2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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