WB (Western Blot)
(Detection of mouse Phospho RNA Polymerase 2 (S2) by western blot. Samples: Whole cell lysate (10 ug) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Phospho RNA Polymerase 2 (S2) antibody (AAA23772 lot 4) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
Rabbit anti-Human, Mouse RNA Polymerase II Polyclonal Antibody | anti-POLR2A antibody
Rabbit anti-Phospho RNA Polymerase II (S2) Antibody, Affinity Purified
IP: 6ug/mg lysate
IHC: 1:500 to 1:2,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of mouse Phospho RNA Polymerase 2 (S2) by western blot. Samples: Whole cell lysate (10 ug) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Phospho RNA Polymerase 2 (S2) antibody (AAA23772 lot 4) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
WB (Western Blot)
(Detection of mouse Phospho RNA Polymerase 2 (S2) by western blot. Samples: Whole cell lysate (10 ug) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Phospho RNA Polymerase 2 (S2) antibody (AAA23772 lot 4) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
WB (Western Blot)
(Detection of human Phospho RNA Polymerase 2 (S2) by western blot. Samples: Whole cell lysate (10 ug) from HeLa, Jurkat, HEK293T, K-562, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Phospho RNA Polymerase 2 (S2) antibody (AAA23772 lot 4) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.)
WB (Western Blot)
(Detection of human Phospho RNA Polymerase 2 (S2) by western blot. Samples: Whole cell lysate (10 ug) from HeLa, Jurkat, HEK293T, K-562, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Phospho RNA Polymerase 2 (S2) antibody (AAA23772 lot 4) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.)
WB (Western Blot)
(Detection of Phospho RNA Polymerase 2 (S2) by peptide dot blot analysis. Samples: 5ug RNA Pol II peptides corresponding to phospho S5, phospho S2, and non-phosphorylated RNA Pol II. Antibody: Affinity purified rabbit anti-Phospho RNA Polymerase 2 (S2) antibody (AAA23772 lot 4) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 min.)
WB (Western Blot)
(Detection of Phospho RNA Polymerase 2 (S2) by peptide dot blot analysis. Samples: 5ug RNA Pol II peptides corresponding to phospho S5, phospho S2, and non-phosphorylated RNA Pol II. Antibody: Affinity purified rabbit anti-Phospho RNA Polymerase 2 (S2) antibody (AAA23772 lot 4) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 min.)
IP (Immunoprecipitation)
(Detection of human Phospho RNA Polymerase 2 (S2) by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg per IP; 10% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Phospho RNA Polymerase 2 (S2) antibody (AAA23772 lot 4) used for IP at 6 ug per reaction. Phospho RNA Polymerase 2 (S2) was also immunoprecipitated by a previous lot of this antibody (AAA23772 lot 3). For blotting immunoprecipitated Phospho RNA Polymerase 2 (S2), AAA23772 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.)
IP (Immunoprecipitation)
(Detection of human Phospho RNA Polymerase 2 (S2) by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg per IP; 10% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Phospho RNA Polymerase 2 (S2) antibody (AAA23772 lot 4) used for IP at 6 ug per reaction. Phospho RNA Polymerase 2 (S2) was also immunoprecipitated by a previous lot of this antibody (AAA23772 lot 3). For blotting immunoprecipitated Phospho RNA Polymerase 2 (S2), AAA23772 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.)
IHC (Immunohistochemistry)
(Detection of human Phospho RNA Polymerase II (S2) by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-Phospho-RNA Polymerase II (S2) (AAA23772). Secondary: HRP-conjugated goat anti-rabbit IgG .)
IHC (Immunohistochemistry)
(Detection of human Phospho RNA Polymerase II (S2) by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-Phospho-RNA Polymerase II (S2) (AAA23772). Secondary: HRP-conjugated goat anti-rabbit IgG .)
IHC (Immunohistochemistry)
(Detection of mouse Phospho RNA Polymerase II (S2) by immunohistochemistry. Sample: FFPE section of mouse intestine. Antibody: Affinity purified rabbit anti-Phospho-RNA Polymerase II (S2) (AAA23772). Secondary: HRP-conjugated goat anti-rabbit IgG .)
IHC (Immunohistochemistry)
(Detection of mouse Phospho RNA Polymerase II (S2) by immunohistochemistry. Sample: FFPE section of mouse intestine. Antibody: Affinity purified rabbit anti-Phospho-RNA Polymerase II (S2) (AAA23772). Secondary: HRP-conjugated goat anti-rabbit IgG .)
NCBI and Uniprot Product Information
Similar Products
Product Notes
The POLR2A polr2a (Catalog #AAA23772) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Phospho RNA Polymerase II (S2) Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's RNA Polymerase II can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC). WB: 1:2000-1:10,000 (3-8% Tris-acetate gel recommended) IP: 6ug/mg lysate IHC: 1:500 to 1:2,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Researchers should empirically determine the suitability of the POLR2A polr2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RNA Polymerase II, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
